Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3454 and Pf1N1B4_3455 are separated by 198 nucleotides Pf1N1B4_3455 and Pf1N1B4_3456 are separated by 411 nucleotides Pf1N1B4_3456 and Pf1N1B4_3457 overlap by 26 nucleotides Pf1N1B4_3457 and Pf1N1B4_3458 are separated by 23 nucleotides Pf1N1B4_3458 and Pf1N1B4_5224 are separated by 292 nucleotides Pf1N1B4_5224 and Pf1N1B4_3459 are separated by 86 nucleotides
Pf1N1B4_3454 - Mg/Co/Ni transporter MgtE / CBS domain, at 3,851,495 to 3,852,937
_3454
Pf1N1B4_3455 - Alginate biosynthesis transcriptional activator, at 3,853,136 to 3,853,462
_3455
Pf1N1B4_3456 - ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8), at 3,853,874 to 3,854,422
_3456
Pf1N1B4_3457 - hypothetical protein, at 3,854,397 to 3,854,576
_3457
Pf1N1B4_3458 - hypothetical protein, at 3,854,600 to 3,854,833
_3458
Pf1N1B4_5224 - tRNA-Arg-CCT, at 3,855,126 to 3,855,199
_5224
Pf1N1B4_3459 - T1SS secreted agglutinin RTX, at 3,855,286 to 3,862,629
_3459
Position (kb)
3853
3854
3855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3853.013 kb on + strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.307 kb on - strand, within Pf1N1B4_3455 at 3853.407 kb on - strand, within Pf1N1B4_3455 at 3853.438 kb on - strand at 3853.438 kb on - strand at 3853.463 kb on - strand at 3853.465 kb on - strand at 3853.520 kb on + strand at 3853.559 kb on + strand at 3853.617 kb on + strand at 3853.617 kb on + strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.637 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.680 kb on + strand at 3853.680 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.758 kb on - strand at 3853.781 kb on + strand at 3853.786 kb on + strand at 3853.786 kb on + strand at 3853.787 kb on - strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.814 kb on + strand at 3853.814 kb on + strand at 3853.814 kb on + strand at 3853.815 kb on - strand at 3853.815 kb on - strand at 3853.818 kb on - strand at 3853.829 kb on - strand at 3853.863 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.869 kb on - strand at 3853.873 kb on - strand at 3854.101 kb on - strand at 3854.188 kb on - strand, within Pf1N1B4_3456 at 3854.188 kb on - strand at 3854.191 kb on - strand, within Pf1N1B4_3456 at 3854.199 kb on + strand, within Pf1N1B4_3456 at 3854.239 kb on - strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.359 kb on + strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.364 kb on + strand, within Pf1N1B4_3456 at 3854.365 kb on - strand, within Pf1N1B4_3456 at 3854.570 kb on + strand at 3854.571 kb on - strand at 3854.571 kb on - strand at 3854.642 kb on - strand, within Pf1N1B4_3458 at 3854.642 kb on - strand at 3854.724 kb on + strand, within Pf1N1B4_3458 at 3854.725 kb on - strand, within Pf1N1B4_3458 at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.030 kb on - strand at 3855.035 kb on + strand at 3855.036 kb on - strand at 3855.036 kb on - strand at 3855.133 kb on + strand at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.147 kb on - strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.163 kb on - strand, within Pf1N1B4_5224 at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.206 kb on - strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,853,013 + +1.5 3,853,110 - +0.4 3,853,110 - -0.1 3,853,110 - +0.2 3,853,110 - -0.1 3,853,307 - Pf1N1B4_3455 0.52 +0.9 3,853,407 - Pf1N1B4_3455 0.83 -2.1 3,853,438 - +0.3 3,853,438 - -0.2 3,853,463 - +2.5 3,853,465 - +1.3 3,853,520 + +0.9 3,853,559 + +1.5 3,853,617 + -1.1 3,853,617 + +0.3 3,853,618 - +0.4 3,853,618 - +1.7 3,853,618 - +1.0 3,853,618 - -0.1 3,853,618 - -0.8 3,853,618 - -2.2 3,853,637 + +1.7 3,853,650 + -0.1 3,853,650 + +2.5 3,853,650 + -0.1 3,853,650 + -0.6 3,853,680 + +1.2 3,853,680 + +0.7 3,853,757 + -0.1 3,853,757 + -1.1 3,853,757 + -0.1 3,853,757 + -1.1 3,853,758 - +1.1 3,853,781 + +1.5 3,853,786 + +0.7 3,853,786 + -0.3 3,853,787 - +3.5 3,853,788 + +0.1 3,853,788 + -0.1 3,853,788 + +2.1 3,853,788 + -0.1 3,853,788 + +1.9 3,853,788 + -0.1 3,853,788 + +0.1 3,853,789 - -0.1 3,853,789 - -1.1 3,853,789 - -0.7 3,853,789 - -0.1 3,853,814 + -1.1 3,853,814 + -0.2 3,853,814 + -1.1 3,853,815 - +0.7 3,853,815 - -2.4 3,853,818 - -0.8 3,853,829 - +0.9 3,853,863 + +0.5 3,853,868 + +0.9 3,853,868 + -0.7 3,853,868 + -0.1 3,853,868 + -0.2 3,853,869 - +0.3 3,853,873 - -0.5 3,854,101 - +1.3 3,854,188 - Pf1N1B4_3456 0.57 -0.1 3,854,188 - +1.5 3,854,191 - Pf1N1B4_3456 0.58 -0.4 3,854,199 + Pf1N1B4_3456 0.59 +0.5 3,854,239 - Pf1N1B4_3456 0.66 -2.2 3,854,323 + Pf1N1B4_3456 0.82 -1.8 3,854,323 + Pf1N1B4_3456 0.82 -2.1 3,854,359 + Pf1N1B4_3456 0.88 -0.1 3,854,360 - Pf1N1B4_3456 0.89 -0.7 3,854,360 - Pf1N1B4_3456 0.89 -1.7 3,854,360 - Pf1N1B4_3456 0.89 -0.8 3,854,360 - Pf1N1B4_3456 0.89 -0.1 3,854,364 + Pf1N1B4_3456 0.89 +0.9 3,854,365 - Pf1N1B4_3456 0.89 -1.1 3,854,570 + +1.5 3,854,571 - +0.9 3,854,571 - +0.3 3,854,642 - Pf1N1B4_3458 0.18 +1.3 3,854,642 - -0.1 3,854,724 + Pf1N1B4_3458 0.53 +1.6 3,854,725 - Pf1N1B4_3458 0.53 -0.2 3,855,029 + -0.9 3,855,029 + -1.1 3,855,029 + -0.5 3,855,029 + -1.7 3,855,030 - +0.9 3,855,035 + -1.1 3,855,036 - -1.4 3,855,036 - -2.1 3,855,133 + -0.1 3,855,146 + Pf1N1B4_5224 0.27 -2.9 3,855,146 + Pf1N1B4_5224 0.27 -2.9 3,855,147 - Pf1N1B4_5224 0.28 -0.1 3,855,162 + Pf1N1B4_5224 0.49 +0.5 3,855,162 + Pf1N1B4_5224 0.49 -2.9 3,855,163 - Pf1N1B4_5224 0.50 -1.1 3,855,205 + +2.9 3,855,205 + -1.7 3,855,205 + -2.4 3,855,205 + +1.3 3,855,205 + -2.4 3,855,205 + +0.7 3,855,206 - +0.7 3,855,300 + +0.1 3,855,300 + -1.1 3,855,300 + -0.1 3,855,300 + +1.5 3,855,300 + -1.5 3,855,301 - -0.3 3,855,301 - -1.1 3,855,301 - -1.8 3,855,301 - -0.1 3,855,387 + -0.8
Or see this region's nucleotide sequence