Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3109 and Pf1N1B4_3110 are separated by 212 nucleotides Pf1N1B4_3110 and Pf1N1B4_3111 overlap by 4 nucleotides Pf1N1B4_3111 and Pf1N1B4_3112 overlap by 13 nucleotides Pf1N1B4_3112 and Pf1N1B4_3113 overlap by 1 nucleotides
Pf1N1B4_3109 - S-formylglutathione hydrolase (EC 3.1.2.12), at 3,496,179 to 3,497,024
_3109
Pf1N1B4_3110 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 3,497,237 to 3,497,710
_3110
Pf1N1B4_3111 - tRNA pseudouridine 13 synthase (EC 4.2.1.-), at 3,497,707 to 3,498,765
_3111
Pf1N1B4_3112 - 5-nucleotidase SurE (EC 3.1.3.5) @ Exopolyphosphatase (EC 3.6.1.11), at 3,498,753 to 3,499,502
_3112
Pf1N1B4_3113 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 3,499,502 to 3,500,179
_3113
Position (kb)
3497
3498
3499 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.959 kb on + strand at 3496.960 kb on - strand at 3497.014 kb on + strand at 3497.022 kb on + strand at 3497.181 kb on + strand at 3497.181 kb on + strand at 3497.182 kb on - strand at 3497.200 kb on + strand at 3497.200 kb on + strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.201 kb on - strand at 3497.741 kb on - strand at 3497.811 kb on + strand at 3497.933 kb on - strand, within Pf1N1B4_3111 at 3498.177 kb on + strand, within Pf1N1B4_3111 at 3498.177 kb on + strand, within Pf1N1B4_3111 at 3498.178 kb on - strand, within Pf1N1B4_3111 at 3498.453 kb on + strand, within Pf1N1B4_3111 at 3498.758 kb on + strand at 3498.810 kb on + strand at 3498.810 kb on + strand at 3499.056 kb on + strand at 3499.056 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.138 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.416 kb on + strand, within Pf1N1B4_3112 at 3499.454 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.522 kb on + strand at 3499.523 kb on - strand at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.586 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.679 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.687 kb on + strand, within Pf1N1B4_3113 at 3499.708 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.730 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113 at 3499.732 kb on + strand, within Pf1N1B4_3113
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,496,959 + +1.0 3,496,959 + -0.8 3,496,959 + +0.0 3,496,959 + -1.6 3,496,959 + -1.6 3,496,959 + -1.3 3,496,959 + -2.0 3,496,959 + +2.8 3,496,960 - -1.6 3,497,014 + -0.8 3,497,022 + +1.0 3,497,181 + -1.6 3,497,181 + +0.0 3,497,182 - +0.4 3,497,200 + -1.6 3,497,200 + +0.0 3,497,201 - -1.6 3,497,201 - +0.0 3,497,201 - +0.0 3,497,741 - -3.1 3,497,811 + -2.0 3,497,933 - Pf1N1B4_3111 0.21 -1.0 3,498,177 + Pf1N1B4_3111 0.44 -2.0 3,498,177 + Pf1N1B4_3111 0.44 +1.0 3,498,178 - Pf1N1B4_3111 0.44 -2.0 3,498,453 + Pf1N1B4_3111 0.70 -1.2 3,498,758 + +0.0 3,498,810 + -1.3 3,498,810 + -0.1 3,499,056 + +0.0 3,499,056 + Pf1N1B4_3112 0.40 +0.3 3,499,138 + Pf1N1B4_3112 0.51 +0.6 3,499,138 + Pf1N1B4_3112 0.51 -1.0 3,499,138 + Pf1N1B4_3112 0.51 +1.3 3,499,416 + Pf1N1B4_3112 0.88 +1.6 3,499,416 + Pf1N1B4_3112 0.88 +0.9 3,499,416 + Pf1N1B4_3112 0.88 +0.0 3,499,416 + Pf1N1B4_3112 0.88 -2.6 3,499,416 + Pf1N1B4_3112 0.88 +0.7 3,499,416 + Pf1N1B4_3112 0.88 +1.2 3,499,416 + Pf1N1B4_3112 0.88 -1.6 3,499,416 + Pf1N1B4_3112 0.88 +1.7 3,499,416 + Pf1N1B4_3112 0.88 +0.8 3,499,416 + Pf1N1B4_3112 0.88 -1.6 3,499,454 + +1.2 3,499,522 + +1.0 3,499,522 + -2.6 3,499,522 + +1.0 3,499,522 + +0.1 3,499,522 + +0.7 3,499,522 + +2.3 3,499,523 - -1.6 3,499,586 + Pf1N1B4_3113 0.12 +2.4 3,499,586 + Pf1N1B4_3113 0.12 +2.5 3,499,679 + Pf1N1B4_3113 0.26 +1.2 3,499,679 + Pf1N1B4_3113 0.26 -1.0 3,499,679 + Pf1N1B4_3113 0.26 +0.0 3,499,687 + Pf1N1B4_3113 0.27 +0.6 3,499,687 + Pf1N1B4_3113 0.27 +1.1 3,499,687 + Pf1N1B4_3113 0.27 +1.8 3,499,708 + Pf1N1B4_3113 0.30 +0.3 3,499,730 + Pf1N1B4_3113 0.34 +0.5 3,499,730 + Pf1N1B4_3113 0.34 +2.1 3,499,730 + Pf1N1B4_3113 0.34 +1.5 3,499,730 + Pf1N1B4_3113 0.34 +2.5 3,499,730 + Pf1N1B4_3113 0.34 +1.5 3,499,730 + Pf1N1B4_3113 0.34 +1.7 3,499,730 + Pf1N1B4_3113 0.34 -1.0 3,499,730 + Pf1N1B4_3113 0.34 +1.4 3,499,730 + Pf1N1B4_3113 0.34 +0.7 3,499,730 + Pf1N1B4_3113 0.34 +1.1 3,499,730 + Pf1N1B4_3113 0.34 +2.1 3,499,730 + Pf1N1B4_3113 0.34 +2.6 3,499,730 + Pf1N1B4_3113 0.34 +1.6 3,499,730 + Pf1N1B4_3113 0.34 +1.7 3,499,730 + Pf1N1B4_3113 0.34 +0.4 3,499,730 + Pf1N1B4_3113 0.34 -1.0 3,499,730 + Pf1N1B4_3113 0.34 +1.9 3,499,730 + Pf1N1B4_3113 0.34 -1.8 3,499,730 + Pf1N1B4_3113 0.34 +1.9 3,499,730 + Pf1N1B4_3113 0.34 +2.2 3,499,730 + Pf1N1B4_3113 0.34 +0.9 3,499,730 + Pf1N1B4_3113 0.34 +0.9 3,499,730 + Pf1N1B4_3113 0.34 +3.0 3,499,730 + Pf1N1B4_3113 0.34 +1.2 3,499,730 + Pf1N1B4_3113 0.34 +1.8 3,499,730 + Pf1N1B4_3113 0.34 +0.9 3,499,730 + Pf1N1B4_3113 0.34 +3.0 3,499,730 + Pf1N1B4_3113 0.34 +1.5 3,499,730 + Pf1N1B4_3113 0.34 +0.7 3,499,730 + Pf1N1B4_3113 0.34 +0.6 3,499,730 + Pf1N1B4_3113 0.34 +1.7 3,499,730 + Pf1N1B4_3113 0.34 +1.0 3,499,730 + Pf1N1B4_3113 0.34 +1.3 3,499,730 + Pf1N1B4_3113 0.34 +1.8 3,499,730 + Pf1N1B4_3113 0.34 -1.0 3,499,730 + Pf1N1B4_3113 0.34 +0.0 3,499,730 + Pf1N1B4_3113 0.34 +1.5 3,499,730 + +0.0 3,499,730 + Pf1N1B4_3113 0.34 +0.3 3,499,730 + Pf1N1B4_3113 0.34 +0.0 3,499,730 + Pf1N1B4_3113 0.34 -0.4 3,499,730 + Pf1N1B4_3113 0.34 +1.8 3,499,730 + Pf1N1B4_3113 0.34 +1.7 3,499,730 + Pf1N1B4_3113 0.34 -1.6 3,499,730 + Pf1N1B4_3113 0.34 -0.7 3,499,730 + Pf1N1B4_3113 0.34 +0.0 3,499,730 + Pf1N1B4_3113 0.34 +2.2 3,499,730 + Pf1N1B4_3113 0.34 +0.9 3,499,732 + Pf1N1B4_3113 0.34 +0.6 3,499,732 + Pf1N1B4_3113 0.34 -2.0 3,499,732 + Pf1N1B4_3113 0.34 +1.4 3,499,732 + Pf1N1B4_3113 0.34 -0.2 3,499,732 + Pf1N1B4_3113 0.34 -1.0 3,499,732 + Pf1N1B4_3113 0.34 +2.3 3,499,732 + Pf1N1B4_3113 0.34 +1.3 3,499,732 + Pf1N1B4_3113 0.34 -0.2 3,499,732 + Pf1N1B4_3113 0.34 +1.2 3,499,732 + +2.3 3,499,732 + Pf1N1B4_3113 0.34 +2.5 3,499,732 + Pf1N1B4_3113 0.34 -0.3 3,499,732 + Pf1N1B4_3113 0.34 -0.1 3,499,732 + Pf1N1B4_3113 0.34 +1.2 3,499,732 + Pf1N1B4_3113 0.34 -0.1 3,499,732 + Pf1N1B4_3113 0.34 +1.4 3,499,732 + Pf1N1B4_3113 0.34 +1.8 3,499,732 + Pf1N1B4_3113 0.34 +0.5 3,499,732 + Pf1N1B4_3113 0.34 +1.3 3,499,732 + Pf1N1B4_3113 0.34 +0.8 3,499,732 + Pf1N1B4_3113 0.34 +1.3 3,499,732 + Pf1N1B4_3113 0.34 -3.6 3,499,732 + Pf1N1B4_3113 0.34 +1.3 3,499,732 + Pf1N1B4_3113 0.34 -1.0 3,499,732 + Pf1N1B4_3113 0.34 +0.8 3,499,732 + Pf1N1B4_3113 0.34 -0.4
Or see this region's nucleotide sequence