Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_308 and Pf1N1B4_309 are separated by 44 nucleotides Pf1N1B4_309 and Pf1N1B4_310 are separated by 245 nucleotides Pf1N1B4_310 and Pf1N1B4_311 are separated by 141 nucleotides Pf1N1B4_311 and Pf1N1B4_312 are separated by 48 nucleotides
Pf1N1B4_308 - FIG00956090: hypothetical protein, at 361,880 to 362,647
_308
Pf1N1B4_309 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 362,692 to 362,970
_309
Pf1N1B4_310 - UPF0028 protein YchK, at 363,216 to 364,256
_310
Pf1N1B4_311 - sensor histidine kinase, at 364,398 to 365,096
_311
Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796
_312
Position (kb) 363
364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.659 kb on + strand at 362.659 kb on + strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.720 kb on + strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.759 kb on + strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.817 kb on - strand, within Pf1N1B4_309 at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand at 363.411 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand, within Pf1N1B4_310 at 363.771 kb on + strand at 364.101 kb on + strand, within Pf1N1B4_310 at 364.102 kb on - strand, within Pf1N1B4_310 at 364.173 kb on + strand at 364.173 kb on + strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.174 kb on - strand at 364.234 kb on - strand at 364.399 kb on + strand at 364.399 kb on + strand at 364.399 kb on + strand at 364.451 kb on + strand at 364.506 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.516 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.618 kb on + strand, within Pf1N1B4_311 at 364.619 kb on - strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.795 kb on + strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.796 kb on - strand, within Pf1N1B4_311 at 364.824 kb on + strand, within Pf1N1B4_311 at 364.824 kb on + strand, within Pf1N1B4_311 at 365.105 kb on - strand at 365.131 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 362,398 - Pf1N1B4_308 0.67 +0.4 362,398 - Pf1N1B4_308 0.67 +0.5 362,398 - Pf1N1B4_308 0.67 +2.5 362,534 + Pf1N1B4_308 0.85 +0.8 362,534 + Pf1N1B4_308 0.85 -1.1 362,535 - Pf1N1B4_308 0.85 -0.1 362,535 - Pf1N1B4_308 0.85 -1.4 362,535 - Pf1N1B4_308 0.85 -1.4 362,535 - Pf1N1B4_308 0.85 +0.3 362,535 - Pf1N1B4_308 0.85 -0.9 362,535 - Pf1N1B4_308 0.85 +0.2 362,659 + +0.5 362,659 + -0.8 362,684 - -0.7 362,684 - -0.2 362,684 - -0.8 362,684 - +0.3 362,684 - +0.2 362,720 + Pf1N1B4_309 0.10 +2.9 362,721 - Pf1N1B4_309 0.10 -0.1 362,721 - Pf1N1B4_309 0.10 -2.1 362,721 - Pf1N1B4_309 0.10 -1.1 362,759 + Pf1N1B4_309 0.24 +0.9 362,760 - Pf1N1B4_309 0.24 +2.8 362,760 - Pf1N1B4_309 0.24 -0.8 362,760 - Pf1N1B4_309 0.24 +1.4 362,760 - Pf1N1B4_309 0.24 +0.3 362,817 - Pf1N1B4_309 0.45 -0.1 362,988 + -0.7 362,988 + +0.1 362,988 + +1.6 362,988 + +0.5 362,992 + -0.8 362,992 + +1.9 362,992 + -1.1 362,992 + +0.9 362,993 - -0.1 362,993 - +0.8 362,993 - -0.7 362,996 + +1.2 362,996 + -1.7 362,996 + +2.2 362,996 + +0.9 362,996 + +0.6 362,996 + +0.4 362,996 + +0.3 362,996 + -0.1 362,996 + -0.1 362,996 + -0.1 362,996 + +0.2 362,996 + +1.2 362,997 - -0.9 362,997 - +1.9 362,997 - -0.7 362,997 - +1.2 362,997 - -0.1 362,997 - +0.8 362,997 - +1.5 362,997 - +0.6 362,997 - -0.3 363,008 + +0.9 363,008 + -0.9 363,008 + -0.5 363,008 + -2.7 363,008 + +1.1 363,009 - -2.4 363,009 - -0.1 363,009 - +0.3 363,009 - +0.0 363,009 - -2.1 363,082 + +1.5 363,082 + -0.1 363,082 + +0.5 363,082 + +0.9 363,083 - -2.1 363,083 - -0.8 363,083 - -1.5 363,083 - -2.4 363,360 + Pf1N1B4_310 0.14 -0.5 363,360 + Pf1N1B4_310 0.14 +0.2 363,360 + Pf1N1B4_310 0.14 -1.1 363,360 + Pf1N1B4_310 0.14 -1.1 363,361 - Pf1N1B4_310 0.14 -0.1 363,361 - Pf1N1B4_310 0.14 +0.0 363,361 - -1.1 363,411 + Pf1N1B4_310 0.19 -1.6 363,509 + Pf1N1B4_310 0.28 -0.1 363,509 + Pf1N1B4_310 0.28 -1.7 363,510 - Pf1N1B4_310 0.28 +1.2 363,510 - Pf1N1B4_310 0.28 -1.1 363,512 + Pf1N1B4_310 0.28 +0.5 363,512 + Pf1N1B4_310 0.28 -0.1 363,512 + Pf1N1B4_310 0.28 +1.1 363,771 + Pf1N1B4_310 0.53 -1.7 363,771 + +1.5 364,101 + Pf1N1B4_310 0.85 +0.5 364,102 - Pf1N1B4_310 0.85 -2.1 364,173 + -0.1 364,173 + -0.1 364,174 - +0.5 364,174 - -1.1 364,174 - -2.1 364,174 - +1.5 364,174 - +1.9 364,234 - +0.6 364,399 + -0.1 364,399 + +0.9 364,399 + +2.5 364,451 + +1.0 364,506 + Pf1N1B4_311 0.15 -0.1 364,516 + Pf1N1B4_311 0.17 +0.4 364,516 + Pf1N1B4_311 0.17 +0.8 364,516 + Pf1N1B4_311 0.17 -1.3 364,516 + Pf1N1B4_311 0.17 +0.7 364,618 + Pf1N1B4_311 0.31 +2.1 364,618 + Pf1N1B4_311 0.31 -1.7 364,618 + Pf1N1B4_311 0.31 +0.7 364,618 + Pf1N1B4_311 0.31 +0.3 364,619 - Pf1N1B4_311 0.32 -0.8 364,795 + Pf1N1B4_311 0.57 -1.0 364,795 + Pf1N1B4_311 0.57 -0.1 364,795 + Pf1N1B4_311 0.57 -1.7 364,795 + Pf1N1B4_311 0.57 -0.3 364,796 - Pf1N1B4_311 0.57 +1.2 364,796 - Pf1N1B4_311 0.57 -1.7 364,796 - Pf1N1B4_311 0.57 -0.6 364,824 + Pf1N1B4_311 0.61 -0.3 364,824 + Pf1N1B4_311 0.61 -1.1 365,105 - +0.5 365,131 + +0.9
Or see this region's nucleotide sequence