Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3097 and Pf1N1B4_3098 overlap by 1 nucleotides Pf1N1B4_3098 and Pf1N1B4_3099 are separated by 82 nucleotides Pf1N1B4_3099 and Pf1N1B4_3100 are separated by 152 nucleotides
Pf1N1B4_3097 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,480,609 to 3,481,736
_3097
Pf1N1B4_3098 - Ribonuclease HII (EC 3.1.26.4), at 3,481,736 to 3,482,377
_3098
Pf1N1B4_3099 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,482,460 to 3,485,981
_3099
Pf1N1B4_3100 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 3,486,134 to 3,487,081
_3100
Position (kb)
3482
3483
3484
3485
3486 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3481.734 kb on + strand at 3481.734 kb on + strand at 3481.735 kb on - strand at 3481.735 kb on - strand at 3481.771 kb on + strand at 3481.791 kb on + strand at 3481.791 kb on + strand at 3481.791 kb on + strand at 3481.792 kb on - strand at 3481.792 kb on - strand at 3481.792 kb on - strand at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.970 kb on + strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.971 kb on - strand, within Pf1N1B4_3098 at 3481.990 kb on - strand, within Pf1N1B4_3098 at 3482.063 kb on - strand, within Pf1N1B4_3098 at 3482.345 kb on + strand at 3482.375 kb on + strand at 3482.394 kb on + strand at 3482.394 kb on + strand at 3482.394 kb on + strand at 3482.439 kb on + strand at 3482.446 kb on + strand at 3486.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,481,734 + +0.3 3,481,734 + -1.0 3,481,735 - -0.5 3,481,735 - -2.3 3,481,771 + -2.0 3,481,791 + +0.2 3,481,791 + -0.7 3,481,791 + +1.6 3,481,792 - +1.0 3,481,792 - -0.7 3,481,792 - +0.0 3,481,970 + Pf1N1B4_3098 0.36 +0.0 3,481,970 + Pf1N1B4_3098 0.36 +2.6 3,481,970 + Pf1N1B4_3098 0.36 +0.6 3,481,970 + Pf1N1B4_3098 0.36 +0.6 3,481,971 - Pf1N1B4_3098 0.37 -0.7 3,481,971 - Pf1N1B4_3098 0.37 -1.6 3,481,971 - Pf1N1B4_3098 0.37 +0.0 3,481,971 - Pf1N1B4_3098 0.37 -2.8 3,481,971 - Pf1N1B4_3098 0.37 +0.2 3,481,990 - Pf1N1B4_3098 0.40 +1.0 3,482,063 - Pf1N1B4_3098 0.51 +1.0 3,482,345 + +0.0 3,482,375 + -0.2 3,482,394 + -0.7 3,482,394 + +1.1 3,482,394 + -0.3 3,482,439 + -0.6 3,482,446 + -1.0 3,486,006 + +0.6
Or see this region's nucleotide sequence