Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3073 and Pf1N1B4_3074 are separated by 282 nucleotides Pf1N1B4_3074 and Pf1N1B4_3075 are separated by 119 nucleotides Pf1N1B4_3075 and Pf1N1B4_3076 overlap by 4 nucleotides
Pf1N1B4_3073 - probable glucosyl transferase, at 3,454,364 to 3,456,955
_3073
Pf1N1B4_3074 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,457,238 to 3,458,053
_3074
Pf1N1B4_3075 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,458,173 to 3,458,577
_3075
Pf1N1B4_3076 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,458,574 to 3,459,779
_3076
Position (kb)
3457
3458
3459 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3456.711 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.955 kb on + strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.956 kb on - strand at 3456.959 kb on + strand at 3456.960 kb on - strand at 3456.960 kb on - strand at 3456.960 kb on - strand at 3457.033 kb on + strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.034 kb on - strand at 3457.101 kb on + strand at 3457.101 kb on + strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.102 kb on - strand at 3457.139 kb on - strand at 3457.146 kb on + strand at 3457.154 kb on + strand at 3457.154 kb on + strand at 3457.154 kb on + strand at 3457.155 kb on - strand at 3457.155 kb on - strand at 3457.155 kb on - strand at 3457.212 kb on + strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.213 kb on - strand at 3457.215 kb on - strand at 3457.215 kb on - strand at 3457.249 kb on + strand at 3457.286 kb on + strand at 3457.286 kb on + strand at 3457.286 kb on + strand at 3457.287 kb on - strand at 3457.577 kb on + strand, within Pf1N1B4_3074 at 3457.578 kb on - strand, within Pf1N1B4_3074 at 3457.823 kb on + strand, within Pf1N1B4_3074 at 3457.823 kb on + strand, within Pf1N1B4_3074 at 3458.200 kb on + strand at 3458.263 kb on - strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.641 kb on + strand at 3458.642 kb on - strand at 3458.642 kb on - strand at 3458.765 kb on + strand, within Pf1N1B4_3076 at 3458.766 kb on - strand, within Pf1N1B4_3076 at 3458.857 kb on + strand, within Pf1N1B4_3076 at 3458.857 kb on + strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3458.858 kb on - strand, within Pf1N1B4_3076 at 3459.008 kb on - strand, within Pf1N1B4_3076
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,456,711 + -2.0 3,456,955 + -2.0 3,456,955 + +0.8 3,456,955 + +1.6 3,456,955 + +0.0 3,456,955 + +0.8 3,456,956 - +0.0 3,456,956 - +0.0 3,456,956 - +1.6 3,456,956 - -0.6 3,456,956 - +1.2 3,456,959 + -0.4 3,456,960 - -1.6 3,456,960 - -0.4 3,456,960 - -2.0 3,457,033 + +1.7 3,457,034 - +0.4 3,457,034 - -1.9 3,457,034 - +0.3 3,457,034 - +0.8 3,457,101 + +0.4 3,457,101 + +0.4 3,457,102 - -2.3 3,457,102 - +0.5 3,457,102 - -3.0 3,457,102 - -1.0 3,457,102 - +0.6 3,457,102 - -2.0 3,457,139 - -1.6 3,457,146 + +0.7 3,457,154 + +0.0 3,457,154 + +2.3 3,457,154 + +1.1 3,457,155 - -0.4 3,457,155 - +1.8 3,457,155 - +2.3 3,457,212 + -3.3 3,457,213 - +1.6 3,457,213 - -1.2 3,457,213 - +0.7 3,457,213 - -1.2 3,457,215 - +0.2 3,457,215 - -0.2 3,457,249 + -1.6 3,457,286 + +0.3 3,457,286 + +0.0 3,457,286 + -2.0 3,457,287 - +0.0 3,457,577 + Pf1N1B4_3074 0.42 -0.7 3,457,578 - Pf1N1B4_3074 0.42 -2.8 3,457,823 + Pf1N1B4_3074 0.72 -0.4 3,457,823 + Pf1N1B4_3074 0.72 +0.0 3,458,200 + -1.6 3,458,263 - -1.0 3,458,641 + -0.2 3,458,641 + +0.0 3,458,641 + +0.8 3,458,641 + +0.3 3,458,642 - +0.2 3,458,642 - +0.8 3,458,765 + Pf1N1B4_3076 0.16 +0.3 3,458,766 - Pf1N1B4_3076 0.16 -3.0 3,458,857 + Pf1N1B4_3076 0.23 +0.2 3,458,857 + Pf1N1B4_3076 0.23 +2.6 3,458,858 - Pf1N1B4_3076 0.24 -1.6 3,458,858 - Pf1N1B4_3076 0.24 -1.2 3,458,858 - Pf1N1B4_3076 0.24 +1.3 3,458,858 - Pf1N1B4_3076 0.24 -0.6 3,458,858 - Pf1N1B4_3076 0.24 -1.6 3,459,008 - Pf1N1B4_3076 0.36 +0.0
Or see this region's nucleotide sequence