Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2920 and Pf1N1B4_2921 overlap by 7 nucleotides Pf1N1B4_2921 and Pf1N1B4_2922 overlap by 8 nucleotides Pf1N1B4_2922 and Pf1N1B4_2923 are separated by 120 nucleotides Pf1N1B4_2923 and Pf1N1B4_2924 are separated by 136 nucleotides
Pf1N1B4_2920 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 3,278,634 to 3,279,464
_2920
Pf1N1B4_2921 - Molybdopterin biosynthesis protein MoeB, at 3,279,458 to 3,280,213
_2921
Pf1N1B4_2922 - Glutamate racemase (EC 5.1.1.3), at 3,280,206 to 3,280,997
_2922
Pf1N1B4_2923 - Lipid A 3-O-deacylase, at 3,281,118 to 3,281,636
_2923
Pf1N1B4_2924 - FIG00955589: hypothetical protein, at 3,281,773 to 3,282,078
_2924
Pf1N1B4_2925 - hypothetical protein, at 3,281,993 to 3,282,115
_2925
Position (kb)
3280
3281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3279.477 kb on + strand at 3279.981 kb on + strand, within Pf1N1B4_2921 at 3279.983 kb on + strand, within Pf1N1B4_2921 at 3279.983 kb on + strand, within Pf1N1B4_2921 at 3280.995 kb on + strand at 3280.996 kb on - strand at 3280.999 kb on + strand at 3281.020 kb on + strand at 3281.055 kb on + strand at 3281.055 kb on + strand at 3281.055 kb on + strand at 3281.056 kb on - strand at 3281.056 kb on - strand at 3281.057 kb on + strand at 3281.057 kb on + strand at 3281.058 kb on - strand at 3281.079 kb on + strand at 3281.079 kb on + strand at 3281.088 kb on + strand at 3281.088 kb on + strand at 3281.089 kb on - strand at 3281.117 kb on - strand at 3281.128 kb on + strand at 3281.235 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.319 kb on + strand, within Pf1N1B4_2923 at 3281.320 kb on - strand, within Pf1N1B4_2923 at 3281.325 kb on + strand, within Pf1N1B4_2923 at 3281.325 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.385 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.415 kb on + strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.563 kb on - strand, within Pf1N1B4_2923 at 3281.599 kb on + strand at 3281.599 kb on + strand at 3281.600 kb on - strand at 3281.600 kb on - strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.607 kb on + strand at 3281.608 kb on - strand at 3281.608 kb on - strand at 3281.619 kb on + strand at 3281.619 kb on + strand at 3281.620 kb on - strand at 3281.634 kb on + strand at 3281.634 kb on + strand at 3281.634 kb on + strand at 3281.642 kb on - strand at 3281.652 kb on + strand at 3281.652 kb on + strand at 3281.652 kb on + strand at 3281.653 kb on - strand at 3281.653 kb on - strand at 3281.766 kb on + strand at 3281.766 kb on + strand at 3281.767 kb on - strand at 3281.864 kb on + strand, within Pf1N1B4_2924 at 3281.864 kb on + strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924 at 3281.865 kb on - strand, within Pf1N1B4_2924
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,279,477 + -0.1 3,279,981 + Pf1N1B4_2921 0.69 -2.5 3,279,983 + Pf1N1B4_2921 0.69 +0.5 3,279,983 + Pf1N1B4_2921 0.69 -1.5 3,280,995 + +1.0 3,280,996 - -1.5 3,280,999 + +0.5 3,281,020 + -1.2 3,281,055 + +0.4 3,281,055 + +0.1 3,281,055 + -2.5 3,281,056 - -0.2 3,281,056 - -0.3 3,281,057 + +2.9 3,281,057 + +1.9 3,281,058 - -0.2 3,281,079 + +1.0 3,281,079 + -0.5 3,281,088 + -0.9 3,281,088 + +1.1 3,281,089 - -0.9 3,281,117 - -0.5 3,281,128 + -0.9 3,281,235 + Pf1N1B4_2923 0.23 +0.7 3,281,319 + Pf1N1B4_2923 0.39 -0.0 3,281,319 + Pf1N1B4_2923 0.39 +0.7 3,281,319 + Pf1N1B4_2923 0.39 +1.7 3,281,319 + Pf1N1B4_2923 0.39 -1.1 3,281,319 + Pf1N1B4_2923 0.39 -0.6 3,281,319 + Pf1N1B4_2923 0.39 -0.1 3,281,320 - Pf1N1B4_2923 0.39 -1.9 3,281,325 + Pf1N1B4_2923 0.40 -0.9 3,281,325 + Pf1N1B4_2923 0.40 -0.1 3,281,385 + Pf1N1B4_2923 0.51 -2.7 3,281,385 + Pf1N1B4_2923 0.51 -2.2 3,281,385 + Pf1N1B4_2923 0.51 +1.7 3,281,415 + Pf1N1B4_2923 0.57 -0.1 3,281,415 + Pf1N1B4_2923 0.57 +1.0 3,281,415 + Pf1N1B4_2923 0.57 -1.0 3,281,563 - Pf1N1B4_2923 0.86 +0.9 3,281,563 - Pf1N1B4_2923 0.86 +0.2 3,281,563 - Pf1N1B4_2923 0.86 -1.7 3,281,599 + +1.1 3,281,599 + -2.9 3,281,600 - +0.4 3,281,600 - +1.1 3,281,607 + +1.1 3,281,607 + +0.1 3,281,607 + -0.9 3,281,607 + +0.1 3,281,608 - -0.5 3,281,608 - -0.6 3,281,619 + -1.9 3,281,619 + -0.5 3,281,620 - -1.9 3,281,634 + +1.1 3,281,634 + +0.3 3,281,634 + -0.9 3,281,642 - +0.3 3,281,652 + -1.2 3,281,652 + +0.1 3,281,652 + -0.9 3,281,653 - -1.5 3,281,653 - -0.9 3,281,766 + +2.0 3,281,766 + +0.1 3,281,767 - +2.3 3,281,864 + Pf1N1B4_2924 0.30 -0.6 3,281,864 + Pf1N1B4_2924 0.30 -0.3 3,281,865 - Pf1N1B4_2924 0.30 +1.1 3,281,865 - Pf1N1B4_2924 0.30 -1.2 3,281,865 - Pf1N1B4_2924 0.30 -0.9
Or see this region's nucleotide sequence