Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2922

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2920 and Pf1N1B4_2921 overlap by 7 nucleotidesPf1N1B4_2921 and Pf1N1B4_2922 overlap by 8 nucleotidesPf1N1B4_2922 and Pf1N1B4_2923 are separated by 120 nucleotidesPf1N1B4_2923 and Pf1N1B4_2924 are separated by 136 nucleotides Pf1N1B4_2920 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 3,278,634 to 3,279,464 _2920 Pf1N1B4_2921 - Molybdopterin biosynthesis protein MoeB, at 3,279,458 to 3,280,213 _2921 Pf1N1B4_2922 - Glutamate racemase (EC 5.1.1.3), at 3,280,206 to 3,280,997 _2922 Pf1N1B4_2923 - Lipid A 3-O-deacylase, at 3,281,118 to 3,281,636 _2923 Pf1N1B4_2924 - FIG00955589: hypothetical protein, at 3,281,773 to 3,282,078 _2924 Pf1N1B4_2925 - hypothetical protein, at 3,281,993 to 3,282,115 _2925 Position (kb) 3280 3281Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3279.477 kb on + strandat 3279.981 kb on + strand, within Pf1N1B4_2921at 3279.983 kb on + strand, within Pf1N1B4_2921at 3279.983 kb on + strand, within Pf1N1B4_2921at 3280.995 kb on + strandat 3280.996 kb on - strandat 3280.999 kb on + strandat 3281.020 kb on + strandat 3281.055 kb on + strandat 3281.055 kb on + strandat 3281.055 kb on + strandat 3281.056 kb on - strandat 3281.056 kb on - strandat 3281.057 kb on + strandat 3281.057 kb on + strandat 3281.058 kb on - strandat 3281.079 kb on + strandat 3281.079 kb on + strandat 3281.088 kb on + strandat 3281.088 kb on + strandat 3281.089 kb on - strandat 3281.117 kb on - strandat 3281.128 kb on + strandat 3281.235 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.319 kb on + strand, within Pf1N1B4_2923at 3281.320 kb on - strand, within Pf1N1B4_2923at 3281.325 kb on + strand, within Pf1N1B4_2923at 3281.325 kb on + strand, within Pf1N1B4_2923at 3281.385 kb on + strand, within Pf1N1B4_2923at 3281.385 kb on + strand, within Pf1N1B4_2923at 3281.385 kb on + strand, within Pf1N1B4_2923at 3281.415 kb on + strand, within Pf1N1B4_2923at 3281.415 kb on + strand, within Pf1N1B4_2923at 3281.415 kb on + strand, within Pf1N1B4_2923at 3281.563 kb on - strand, within Pf1N1B4_2923at 3281.563 kb on - strand, within Pf1N1B4_2923at 3281.563 kb on - strand, within Pf1N1B4_2923at 3281.599 kb on + strandat 3281.599 kb on + strandat 3281.600 kb on - strandat 3281.600 kb on - strandat 3281.607 kb on + strandat 3281.607 kb on + strandat 3281.607 kb on + strandat 3281.607 kb on + strandat 3281.608 kb on - strandat 3281.608 kb on - strandat 3281.619 kb on + strandat 3281.619 kb on + strandat 3281.620 kb on - strandat 3281.634 kb on + strandat 3281.634 kb on + strandat 3281.634 kb on + strandat 3281.642 kb on - strandat 3281.652 kb on + strandat 3281.652 kb on + strandat 3281.652 kb on + strandat 3281.653 kb on - strandat 3281.653 kb on - strandat 3281.766 kb on + strandat 3281.766 kb on + strandat 3281.767 kb on - strandat 3281.864 kb on + strand, within Pf1N1B4_2924at 3281.864 kb on + strand, within Pf1N1B4_2924at 3281.865 kb on - strand, within Pf1N1B4_2924at 3281.865 kb on - strand, within Pf1N1B4_2924at 3281.865 kb on - strand, within Pf1N1B4_2924

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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3,279,477 + -0.1
3,279,981 + Pf1N1B4_2921 0.69 -2.5
3,279,983 + Pf1N1B4_2921 0.69 +0.5
3,279,983 + Pf1N1B4_2921 0.69 -1.5
3,280,995 + +1.0
3,280,996 - -1.5
3,280,999 + +0.5
3,281,020 + -1.2
3,281,055 + +0.4
3,281,055 + +0.1
3,281,055 + -2.5
3,281,056 - -0.2
3,281,056 - -0.3
3,281,057 + +2.9
3,281,057 + +1.9
3,281,058 - -0.2
3,281,079 + +1.0
3,281,079 + -0.5
3,281,088 + -0.9
3,281,088 + +1.1
3,281,089 - -0.9
3,281,117 - -0.5
3,281,128 + -0.9
3,281,235 + Pf1N1B4_2923 0.23 +0.7
3,281,319 + Pf1N1B4_2923 0.39 -0.0
3,281,319 + Pf1N1B4_2923 0.39 +0.7
3,281,319 + Pf1N1B4_2923 0.39 +1.7
3,281,319 + Pf1N1B4_2923 0.39 -1.1
3,281,319 + Pf1N1B4_2923 0.39 -0.6
3,281,319 + Pf1N1B4_2923 0.39 -0.1
3,281,320 - Pf1N1B4_2923 0.39 -1.9
3,281,325 + Pf1N1B4_2923 0.40 -0.9
3,281,325 + Pf1N1B4_2923 0.40 -0.1
3,281,385 + Pf1N1B4_2923 0.51 -2.7
3,281,385 + Pf1N1B4_2923 0.51 -2.2
3,281,385 + Pf1N1B4_2923 0.51 +1.7
3,281,415 + Pf1N1B4_2923 0.57 -0.1
3,281,415 + Pf1N1B4_2923 0.57 +1.0
3,281,415 + Pf1N1B4_2923 0.57 -1.0
3,281,563 - Pf1N1B4_2923 0.86 +0.9
3,281,563 - Pf1N1B4_2923 0.86 +0.2
3,281,563 - Pf1N1B4_2923 0.86 -1.7
3,281,599 + +1.1
3,281,599 + -2.9
3,281,600 - +0.4
3,281,600 - +1.1
3,281,607 + +1.1
3,281,607 + +0.1
3,281,607 + -0.9
3,281,607 + +0.1
3,281,608 - -0.5
3,281,608 - -0.6
3,281,619 + -1.9
3,281,619 + -0.5
3,281,620 - -1.9
3,281,634 + +1.1
3,281,634 + +0.3
3,281,634 + -0.9
3,281,642 - +0.3
3,281,652 + -1.2
3,281,652 + +0.1
3,281,652 + -0.9
3,281,653 - -1.5
3,281,653 - -0.9
3,281,766 + +2.0
3,281,766 + +0.1
3,281,767 - +2.3
3,281,864 + Pf1N1B4_2924 0.30 -0.6
3,281,864 + Pf1N1B4_2924 0.30 -0.3
3,281,865 - Pf1N1B4_2924 0.30 +1.1
3,281,865 - Pf1N1B4_2924 0.30 -1.2
3,281,865 - Pf1N1B4_2924 0.30 -0.9

Or see this region's nucleotide sequence