Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2889 and Pf1N1B4_2890 are separated by 101 nucleotides Pf1N1B4_2890 and Pf1N1B4_2891 overlap by 8 nucleotides Pf1N1B4_2891 and Pf1N1B4_2892 are separated by 101 nucleotides Pf1N1B4_2892 and Pf1N1B4_2893 are separated by 183 nucleotides
Pf1N1B4_2889 - FIG00954491: hypothetical protein, at 3,243,701 to 3,245,044
_2889
Pf1N1B4_2890 - Periplasmic protein TonB, links inner and outer membranes, at 3,245,146 to 3,245,925
_2890
Pf1N1B4_2891 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 3,245,918 to 3,246,370
_2891
Pf1N1B4_2892 - Phosphotransferase system, HPr-related proteins, at 3,246,472 to 3,246,876
_2892
Pf1N1B4_2893 - Carbonic anhydrase (EC 4.2.1.1), at 3,247,060 to 3,247,704
_2893
Position (kb)
3245
3246
3247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3245.004 kb on - strand at 3245.018 kb on + strand at 3245.018 kb on + strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.069 kb on + strand at 3245.070 kb on - strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.931 kb on + strand at 3245.970 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand at 3246.041 kb on + strand, within Pf1N1B4_2891 at 3246.042 kb on - strand, within Pf1N1B4_2891 at 3246.200 kb on + strand, within Pf1N1B4_2891 at 3246.201 kb on - strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.894 kb on + strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.901 kb on + strand at 3246.902 kb on - strand at 3246.904 kb on + strand at 3246.905 kb on - strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.986 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.071 kb on - strand at 3247.071 kb on - strand at 3247.086 kb on + strand at 3247.145 kb on + strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.252 kb on - strand, within Pf1N1B4_2893 at 3247.307 kb on - strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,245,004 - -0.5 3,245,018 + -1.5 3,245,018 + -1.9 3,245,019 - -2.2 3,245,019 - +1.0 3,245,019 - +1.5 3,245,069 + +0.1 3,245,070 - -0.6 3,245,122 + +0.1 3,245,122 + -1.5 3,245,122 + -2.2 3,245,931 + +1.1 3,245,970 + Pf1N1B4_2891 0.11 -2.4 3,245,993 + Pf1N1B4_2891 0.17 -1.2 3,245,993 + -0.9 3,246,041 + Pf1N1B4_2891 0.27 -2.9 3,246,042 - Pf1N1B4_2891 0.27 -0.1 3,246,200 + Pf1N1B4_2891 0.62 +1.7 3,246,201 - Pf1N1B4_2891 0.62 +0.1 3,246,266 + Pf1N1B4_2891 0.77 +1.1 3,246,266 + Pf1N1B4_2891 0.77 -2.2 3,246,266 + Pf1N1B4_2891 0.77 -2.0 3,246,355 + -0.8 3,246,355 + +0.1 3,246,355 + -1.9 3,246,355 + -1.5 3,246,355 + -0.5 3,246,355 + -0.4 3,246,355 + -0.5 3,246,355 + -1.2 3,246,355 + -0.6 3,246,355 + -1.1 3,246,356 - +0.1 3,246,356 - +0.1 3,246,356 - +0.7 3,246,356 - +0.7 3,246,356 - +2.7 3,246,356 - -1.9 3,246,356 - -0.6 3,246,356 - +0.3 3,246,890 - +0.5 3,246,890 - +0.5 3,246,890 - -1.2 3,246,890 - +1.9 3,246,890 - -0.7 3,246,890 - -1.9 3,246,894 + -0.9 3,246,895 - +1.5 3,246,895 - +2.9 3,246,895 - +2.5 3,246,901 + +0.4 3,246,902 - +0.4 3,246,904 + +0.5 3,246,905 - -0.5 3,246,964 + +0.3 3,246,964 + +1.1 3,246,964 + -2.2 3,246,965 - +1.9 3,246,965 - +0.1 3,246,965 - +1.1 3,246,965 - -0.3 3,246,986 + +1.5 3,247,070 + +0.4 3,247,070 + -1.2 3,247,070 + +1.5 3,247,071 - -1.9 3,247,071 - -1.5 3,247,086 + -0.3 3,247,145 + Pf1N1B4_2893 0.13 +1.7 3,247,146 - Pf1N1B4_2893 0.13 +1.4 3,247,146 - Pf1N1B4_2893 0.13 -2.0 3,247,168 + Pf1N1B4_2893 0.17 +0.1 3,247,168 + Pf1N1B4_2893 0.17 -0.1 3,247,168 + Pf1N1B4_2893 0.17 -2.2 3,247,252 - Pf1N1B4_2893 0.30 -1.5 3,247,307 - Pf1N1B4_2893 0.38 +1.5 3,247,370 + Pf1N1B4_2893 0.48 +1.7 3,247,370 + Pf1N1B4_2893 0.48 -1.9
Or see this region's nucleotide sequence