Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2887 and Pf1N1B4_2888 overlap by 4 nucleotides Pf1N1B4_2888 and Pf1N1B4_2889 overlap by 11 nucleotides Pf1N1B4_2889 and Pf1N1B4_2890 are separated by 101 nucleotides Pf1N1B4_2890 and Pf1N1B4_2891 overlap by 8 nucleotides
Pf1N1B4_2887 - Chromosome segregation ATPases, at 3,240,173 to 3,243,013
_2887
Pf1N1B4_2888 - FIG00961017: hypothetical protein, at 3,243,010 to 3,243,711
_2888
Pf1N1B4_2889 - FIG00954491: hypothetical protein, at 3,243,701 to 3,245,044
_2889
Pf1N1B4_2890 - Periplasmic protein TonB, links inner and outer membranes, at 3,245,146 to 3,245,925
_2890
Pf1N1B4_2891 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 3,245,918 to 3,246,370
_2891
Position (kb)
3243
3244
3245
3246 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3242.738 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.799 kb on + strand at 3242.800 kb on - strand at 3242.800 kb on - strand at 3242.826 kb on + strand at 3242.826 kb on + strand at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.497 kb on - strand, within Pf1N1B4_2888 at 3243.497 kb on - strand at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.530 kb on + strand, within Pf1N1B4_2888 at 3243.531 kb on - strand, within Pf1N1B4_2888 at 3243.582 kb on + strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.697 kb on + strand at 3243.698 kb on - strand at 3243.698 kb on - strand at 3244.057 kb on - strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.063 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.471 kb on - strand, within Pf1N1B4_2889 at 3244.644 kb on + strand, within Pf1N1B4_2889 at 3244.645 kb on - strand, within Pf1N1B4_2889 at 3244.674 kb on + strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889 at 3244.722 kb on + strand, within Pf1N1B4_2889 at 3244.723 kb on - strand, within Pf1N1B4_2889 at 3245.004 kb on - strand at 3245.018 kb on + strand at 3245.018 kb on + strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.069 kb on + strand at 3245.070 kb on - strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.931 kb on + strand at 3245.970 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand at 3246.041 kb on + strand, within Pf1N1B4_2891 at 3246.042 kb on - strand, within Pf1N1B4_2891
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,242,738 + -1.3 3,242,778 + -0.2 3,242,778 + -0.9 3,242,778 + +0.5 3,242,778 + -0.9 3,242,778 + -2.4 3,242,779 - -0.9 3,242,779 - -1.9 3,242,779 - -1.3 3,242,779 - +1.1 3,242,779 - -0.6 3,242,779 - -1.5 3,242,779 - -0.9 3,242,779 - -0.9 3,242,779 - -0.3 3,242,779 - -2.9 3,242,799 + -0.9 3,242,800 - -2.9 3,242,800 - +0.1 3,242,826 + -0.1 3,242,826 + -1.5 3,243,252 - Pf1N1B4_2888 0.34 +1.1 3,243,252 - Pf1N1B4_2888 0.34 +0.1 3,243,359 + Pf1N1B4_2888 0.50 -2.2 3,243,359 + Pf1N1B4_2888 0.50 -2.2 3,243,497 - Pf1N1B4_2888 0.69 -2.9 3,243,497 - -1.9 3,243,528 - Pf1N1B4_2888 0.74 -0.9 3,243,528 - Pf1N1B4_2888 0.74 -2.0 3,243,528 - Pf1N1B4_2888 0.74 +1.5 3,243,530 + Pf1N1B4_2888 0.74 -1.1 3,243,531 - Pf1N1B4_2888 0.74 -1.9 3,243,582 + Pf1N1B4_2888 0.81 -3.0 3,243,583 - Pf1N1B4_2888 0.82 -2.2 3,243,583 - Pf1N1B4_2888 0.82 -1.9 3,243,697 + -0.2 3,243,698 - +0.1 3,243,698 - +1.7 3,244,057 - Pf1N1B4_2889 0.26 -1.5 3,244,062 + Pf1N1B4_2889 0.27 +0.1 3,244,062 + Pf1N1B4_2889 0.27 -0.2 3,244,062 + Pf1N1B4_2889 0.27 +1.1 3,244,063 - Pf1N1B4_2889 0.27 +0.0 3,244,441 - Pf1N1B4_2889 0.55 -1.9 3,244,441 - Pf1N1B4_2889 0.55 -1.5 3,244,471 - Pf1N1B4_2889 0.57 -1.9 3,244,644 + Pf1N1B4_2889 0.70 -0.9 3,244,645 - Pf1N1B4_2889 0.70 -1.3 3,244,674 + Pf1N1B4_2889 0.72 -1.5 3,244,675 - Pf1N1B4_2889 0.72 +0.1 3,244,675 - Pf1N1B4_2889 0.72 -1.2 3,244,722 + Pf1N1B4_2889 0.76 -2.2 3,244,723 - Pf1N1B4_2889 0.76 -0.5 3,245,004 - -0.5 3,245,018 + -1.5 3,245,018 + -1.9 3,245,019 - -2.2 3,245,019 - +1.0 3,245,019 - +1.5 3,245,069 + +0.1 3,245,070 - -0.6 3,245,122 + +0.1 3,245,122 + -1.5 3,245,122 + -2.2 3,245,931 + +1.1 3,245,970 + Pf1N1B4_2891 0.11 -2.4 3,245,993 + Pf1N1B4_2891 0.17 -1.2 3,245,993 + -0.9 3,246,041 + Pf1N1B4_2891 0.27 -2.9 3,246,042 - Pf1N1B4_2891 0.27 -0.1
Or see this region's nucleotide sequence