Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_259

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_257 and Pf1N1B4_258 overlap by 17 nucleotidesPf1N1B4_258 and Pf1N1B4_259 overlap by 4 nucleotidesPf1N1B4_259 and Pf1N1B4_260 are separated by 187 nucleotidesPf1N1B4_260 and Pf1N1B4_261 are separated by 226 nucleotides Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841 _257 Pf1N1B4_258 - Organic hydroperoxide resistance transcriptional regulator, at 318,825 to 319,286 _258 Pf1N1B4_259 - Glutathione peroxidase family protein, at 319,283 to 319,768 _259 Pf1N1B4_260 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 319,956 to 320,351 _260 Pf1N1B4_261 - Aspartate aminotransferase (EC 2.6.1.1), at 320,578 to 321,789 _261 Position (kb) 319 320Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 318.384 kb on - strand, within Pf1N1B4_257at 318.384 kb on - strand, within Pf1N1B4_257at 318.645 kb on - strandat 318.826 kb on + strandat 318.913 kb on - strand, within Pf1N1B4_258at 319.157 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.191 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.199 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.229 kb on - strand, within Pf1N1B4_258at 319.280 kb on + strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.281 kb on - strandat 319.509 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.611 kb on + strand, within Pf1N1B4_259at 319.612 kb on - strand, within Pf1N1B4_259at 319.641 kb on + strand, within Pf1N1B4_259at 319.664 kb on - strand, within Pf1N1B4_259at 319.857 kb on + strandat 319.857 kb on + strandat 319.958 kb on - strandat 319.958 kb on - strandat 319.999 kb on + strand, within Pf1N1B4_260at 320.000 kb on - strand, within Pf1N1B4_260at 320.140 kb on + strand, within Pf1N1B4_260at 320.141 kb on - strand, within Pf1N1B4_260at 320.141 kb on - strand, within Pf1N1B4_260at 320.245 kb on + strand, within Pf1N1B4_260at 320.245 kb on + strandat 320.245 kb on + strand, within Pf1N1B4_260at 320.293 kb on + strand, within Pf1N1B4_260at 320.294 kb on - strand, within Pf1N1B4_260at 320.294 kb on - strand, within Pf1N1B4_260at 320.355 kb on + strandat 320.355 kb on + strandat 320.355 kb on + strandat 320.356 kb on - strandat 320.356 kb on - strandat 320.356 kb on - strandat 320.356 kb on - strandat 320.386 kb on + strandat 320.413 kb on + strandat 320.413 kb on + strandat 320.413 kb on + strandat 320.413 kb on + strandat 320.413 kb on + strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.414 kb on - strandat 320.473 kb on + strandat 320.473 kb on + strandat 320.473 kb on + strandat 320.473 kb on + strandat 320.474 kb on - strandat 320.474 kb on - strandat 320.474 kb on - strandat 320.528 kb on - strandat 320.528 kb on - strandat 320.528 kb on - strandat 320.539 kb on + strandat 320.540 kb on - strandat 320.540 kb on - strandat 320.540 kb on - strandat 320.540 kb on - strandat 320.617 kb on + strandat 320.618 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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318,384 - Pf1N1B4_257 0.81 +0.1
318,384 - Pf1N1B4_257 0.81 -1.2
318,645 - +0.4
318,826 + +1.2
318,913 - Pf1N1B4_258 0.19 -0.3
319,157 - Pf1N1B4_258 0.72 +0.5
319,191 - Pf1N1B4_258 0.79 +0.2
319,191 - Pf1N1B4_258 0.79 -0.2
319,191 - Pf1N1B4_258 0.79 +0.8
319,191 - Pf1N1B4_258 0.79 -0.8
319,191 - Pf1N1B4_258 0.79 -0.2
319,191 - Pf1N1B4_258 0.79 -2.8
319,191 - Pf1N1B4_258 0.79 +1.1
319,191 - Pf1N1B4_258 0.79 +1.6
319,191 - Pf1N1B4_258 0.79 -0.8
319,191 - Pf1N1B4_258 0.79 -0.6
319,199 - Pf1N1B4_258 0.81 +0.3
319,199 - Pf1N1B4_258 0.81 -1.5
319,199 - Pf1N1B4_258 0.81 -0.6
319,199 - Pf1N1B4_258 0.81 +0.2
319,229 - Pf1N1B4_258 0.87 +1.4
319,229 - Pf1N1B4_258 0.87 -0.7
319,229 - Pf1N1B4_258 0.87 +2.1
319,229 - Pf1N1B4_258 0.87 +1.8
319,229 - Pf1N1B4_258 0.87 -0.1
319,280 + +1.4
319,281 - -0.5
319,281 - -0.0
319,281 - +3.0
319,281 - -0.4
319,281 - +1.1
319,281 - -0.4
319,281 - +1.4
319,509 + Pf1N1B4_259 0.47 -1.2
319,611 + Pf1N1B4_259 0.67 -2.5
319,611 + Pf1N1B4_259 0.67 +3.0
319,611 + Pf1N1B4_259 0.67 -1.8
319,612 - Pf1N1B4_259 0.68 -2.5
319,641 + Pf1N1B4_259 0.74 +0.2
319,664 - Pf1N1B4_259 0.78 -0.9
319,857 + -0.4
319,857 + +0.2
319,958 - +0.0
319,958 - -1.5
319,999 + Pf1N1B4_260 0.11 +0.8
320,000 - Pf1N1B4_260 0.11 -0.2
320,140 + Pf1N1B4_260 0.46 -0.4
320,141 - Pf1N1B4_260 0.47 +0.1
320,141 - Pf1N1B4_260 0.47 -1.6
320,245 + Pf1N1B4_260 0.73 +0.7
320,245 + -0.7
320,245 + Pf1N1B4_260 0.73 +0.4
320,293 + Pf1N1B4_260 0.85 +0.3
320,294 - Pf1N1B4_260 0.85 +0.0
320,294 - Pf1N1B4_260 0.85 +0.3
320,355 + +0.0
320,355 + -0.8
320,355 + -1.5
320,356 - -0.2
320,356 - -0.2
320,356 - +0.8
320,356 - -1.0
320,386 + +1.3
320,413 + +0.5
320,413 + -2.2
320,413 + +0.2
320,413 + -0.4
320,413 + -1.2
320,414 - +1.4
320,414 - +1.8
320,414 - +1.6
320,414 - -0.3
320,414 - -0.7
320,414 - +2.2
320,414 - -1.0
320,414 - +1.8
320,414 - -1.7
320,414 - +0.3
320,473 + -1.2
320,473 + -0.4
320,473 + +0.3
320,473 + -1.5
320,474 - +1.1
320,474 - -2.2
320,474 - -2.2
320,528 - -1.1
320,528 - +0.6
320,528 - +0.6
320,539 + +0.2
320,540 - -1.6
320,540 - -2.5
320,540 - +0.0
320,540 - +0.1
320,617 + +0.5
320,618 - -0.7

Or see this region's nucleotide sequence