Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_257 and Pf1N1B4_258 overlap by 17 nucleotides Pf1N1B4_258 and Pf1N1B4_259 overlap by 4 nucleotides Pf1N1B4_259 and Pf1N1B4_260 are separated by 187 nucleotides Pf1N1B4_260 and Pf1N1B4_261 are separated by 226 nucleotides
Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841
_257
Pf1N1B4_258 - Organic hydroperoxide resistance transcriptional regulator, at 318,825 to 319,286
_258
Pf1N1B4_259 - Glutathione peroxidase family protein, at 319,283 to 319,768
_259
Pf1N1B4_260 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 319,956 to 320,351
_260
Pf1N1B4_261 - Aspartate aminotransferase (EC 2.6.1.1), at 320,578 to 321,789
_261
Position (kb)
319
320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 318.384 kb on - strand, within Pf1N1B4_257 at 318.384 kb on - strand, within Pf1N1B4_257 at 318.645 kb on - strand at 318.826 kb on + strand at 318.913 kb on - strand, within Pf1N1B4_258 at 319.157 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.191 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.199 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.229 kb on - strand, within Pf1N1B4_258 at 319.280 kb on + strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.281 kb on - strand at 319.509 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.611 kb on + strand, within Pf1N1B4_259 at 319.612 kb on - strand, within Pf1N1B4_259 at 319.641 kb on + strand, within Pf1N1B4_259 at 319.664 kb on - strand, within Pf1N1B4_259 at 319.857 kb on + strand at 319.857 kb on + strand at 319.958 kb on - strand at 319.958 kb on - strand at 319.999 kb on + strand, within Pf1N1B4_260 at 320.000 kb on - strand, within Pf1N1B4_260 at 320.140 kb on + strand, within Pf1N1B4_260 at 320.141 kb on - strand, within Pf1N1B4_260 at 320.141 kb on - strand, within Pf1N1B4_260 at 320.245 kb on + strand, within Pf1N1B4_260 at 320.245 kb on + strand at 320.245 kb on + strand, within Pf1N1B4_260 at 320.293 kb on + strand, within Pf1N1B4_260 at 320.294 kb on - strand, within Pf1N1B4_260 at 320.294 kb on - strand, within Pf1N1B4_260 at 320.355 kb on + strand at 320.355 kb on + strand at 320.355 kb on + strand at 320.356 kb on - strand at 320.356 kb on - strand at 320.356 kb on - strand at 320.356 kb on - strand at 320.386 kb on + strand at 320.413 kb on + strand at 320.413 kb on + strand at 320.413 kb on + strand at 320.413 kb on + strand at 320.413 kb on + strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.414 kb on - strand at 320.473 kb on + strand at 320.473 kb on + strand at 320.473 kb on + strand at 320.473 kb on + strand at 320.474 kb on - strand at 320.474 kb on - strand at 320.474 kb on - strand at 320.528 kb on - strand at 320.528 kb on - strand at 320.528 kb on - strand at 320.539 kb on + strand at 320.540 kb on - strand at 320.540 kb on - strand at 320.540 kb on - strand at 320.540 kb on - strand at 320.617 kb on + strand at 320.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 318,384 - Pf1N1B4_257 0.81 +0.1 318,384 - Pf1N1B4_257 0.81 -1.2 318,645 - +0.4 318,826 + +1.2 318,913 - Pf1N1B4_258 0.19 -0.3 319,157 - Pf1N1B4_258 0.72 +0.5 319,191 - Pf1N1B4_258 0.79 +0.2 319,191 - Pf1N1B4_258 0.79 -0.2 319,191 - Pf1N1B4_258 0.79 +0.8 319,191 - Pf1N1B4_258 0.79 -0.8 319,191 - Pf1N1B4_258 0.79 -0.2 319,191 - Pf1N1B4_258 0.79 -2.8 319,191 - Pf1N1B4_258 0.79 +1.1 319,191 - Pf1N1B4_258 0.79 +1.6 319,191 - Pf1N1B4_258 0.79 -0.8 319,191 - Pf1N1B4_258 0.79 -0.6 319,199 - Pf1N1B4_258 0.81 +0.3 319,199 - Pf1N1B4_258 0.81 -1.5 319,199 - Pf1N1B4_258 0.81 -0.6 319,199 - Pf1N1B4_258 0.81 +0.2 319,229 - Pf1N1B4_258 0.87 +1.4 319,229 - Pf1N1B4_258 0.87 -0.7 319,229 - Pf1N1B4_258 0.87 +2.1 319,229 - Pf1N1B4_258 0.87 +1.8 319,229 - Pf1N1B4_258 0.87 -0.1 319,280 + +1.4 319,281 - -0.5 319,281 - -0.0 319,281 - +3.0 319,281 - -0.4 319,281 - +1.1 319,281 - -0.4 319,281 - +1.4 319,509 + Pf1N1B4_259 0.47 -1.2 319,611 + Pf1N1B4_259 0.67 -2.5 319,611 + Pf1N1B4_259 0.67 +3.0 319,611 + Pf1N1B4_259 0.67 -1.8 319,612 - Pf1N1B4_259 0.68 -2.5 319,641 + Pf1N1B4_259 0.74 +0.2 319,664 - Pf1N1B4_259 0.78 -0.9 319,857 + -0.4 319,857 + +0.2 319,958 - +0.0 319,958 - -1.5 319,999 + Pf1N1B4_260 0.11 +0.8 320,000 - Pf1N1B4_260 0.11 -0.2 320,140 + Pf1N1B4_260 0.46 -0.4 320,141 - Pf1N1B4_260 0.47 +0.1 320,141 - Pf1N1B4_260 0.47 -1.6 320,245 + Pf1N1B4_260 0.73 +0.7 320,245 + -0.7 320,245 + Pf1N1B4_260 0.73 +0.4 320,293 + Pf1N1B4_260 0.85 +0.3 320,294 - Pf1N1B4_260 0.85 +0.0 320,294 - Pf1N1B4_260 0.85 +0.3 320,355 + +0.0 320,355 + -0.8 320,355 + -1.5 320,356 - -0.2 320,356 - -0.2 320,356 - +0.8 320,356 - -1.0 320,386 + +1.3 320,413 + +0.5 320,413 + -2.2 320,413 + +0.2 320,413 + -0.4 320,413 + -1.2 320,414 - +1.4 320,414 - +1.8 320,414 - +1.6 320,414 - -0.3 320,414 - -0.7 320,414 - +2.2 320,414 - -1.0 320,414 - +1.8 320,414 - -1.7 320,414 - +0.3 320,473 + -1.2 320,473 + -0.4 320,473 + +0.3 320,473 + -1.5 320,474 - +1.1 320,474 - -2.2 320,474 - -2.2 320,528 - -1.1 320,528 - +0.6 320,528 - +0.6 320,539 + +0.2 320,540 - -1.6 320,540 - -2.5 320,540 - +0.0 320,540 - +0.1 320,617 + +0.5 320,618 - -0.7
Or see this region's nucleotide sequence