Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2517

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2516 and Pf1N1B4_2517 are separated by 23 nucleotidesPf1N1B4_2517 and Pf1N1B4_2518 are separated by 74 nucleotidesPf1N1B4_2518 and Pf1N1B4_2519 overlap by 10 nucleotides Pf1N1B4_2516 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 2,855,695 to 2,856,720 _2516 Pf1N1B4_2517 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 2,856,744 to 2,857,313 _2517 Pf1N1B4_2518 - Dihydroneopterin aldolase (EC 4.1.2.25), at 2,857,388 to 2,857,744 _2518 Pf1N1B4_2519 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 2,857,735 to 2,858,253 _2519 Position (kb) 2856 2857 2858Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2856.741 kb on + strandat 2856.746 kb on - strandat 2856.780 kb on + strandat 2856.780 kb on + strandat 2856.780 kb on + strandat 2856.781 kb on - strandat 2856.834 kb on + strand, within Pf1N1B4_2517at 2856.919 kb on + strand, within Pf1N1B4_2517at 2856.919 kb on + strand, within Pf1N1B4_2517at 2856.919 kb on + strand, within Pf1N1B4_2517at 2856.920 kb on - strand, within Pf1N1B4_2517at 2856.920 kb on - strand, within Pf1N1B4_2517at 2856.967 kb on + strand, within Pf1N1B4_2517at 2857.027 kb on + strand, within Pf1N1B4_2517at 2857.083 kb on + strand, within Pf1N1B4_2517at 2857.083 kb on + strand, within Pf1N1B4_2517at 2857.083 kb on + strand, within Pf1N1B4_2517at 2857.095 kb on + strand, within Pf1N1B4_2517at 2857.096 kb on - strand, within Pf1N1B4_2517at 2857.316 kb on + strandat 2857.316 kb on + strandat 2857.316 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2857.320 kb on + strandat 2858.288 kb on + strandat 2858.288 kb on + strandat 2858.313 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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2,856,741 + +0.2
2,856,746 - -1.1
2,856,780 + -1.6
2,856,780 + +0.0
2,856,780 + -0.2
2,856,781 - +2.0
2,856,834 + Pf1N1B4_2517 0.16 -3.2
2,856,919 + Pf1N1B4_2517 0.31 -2.1
2,856,919 + Pf1N1B4_2517 0.31 +0.6
2,856,919 + Pf1N1B4_2517 0.31 -0.8
2,856,920 - Pf1N1B4_2517 0.31 -0.1
2,856,920 - Pf1N1B4_2517 0.31 +1.2
2,856,967 + Pf1N1B4_2517 0.39 +0.0
2,857,027 + Pf1N1B4_2517 0.50 -1.2
2,857,083 + Pf1N1B4_2517 0.59 -0.7
2,857,083 + Pf1N1B4_2517 0.59 -0.4
2,857,083 + Pf1N1B4_2517 0.59 -1.4
2,857,095 + Pf1N1B4_2517 0.62 -1.8
2,857,096 - Pf1N1B4_2517 0.62 -0.8
2,857,316 + -0.8
2,857,316 + -2.1
2,857,316 + -0.4
2,857,320 + +0.9
2,857,320 + -2.8
2,857,320 + -0.4
2,857,320 + -0.1
2,857,320 + +0.2
2,857,320 + -0.5
2,857,320 + -0.8
2,858,288 + -0.8
2,858,288 + +0.8
2,858,313 + +0.2

Or see this region's nucleotide sequence