Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides Pf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotides Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides Pf1N1B4_2492 and Pf1N1B4_2493 are separated by 277 nucleotides
Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083
_2489
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Pf1N1B4_2493 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,825,260 to 2,827,065
_2493
Position (kb)
2823
2824
2825 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2823.070 kb on + strand at 2823.070 kb on + strand at 2823.071 kb on - strand at 2823.071 kb on - strand at 2823.086 kb on + strand at 2823.086 kb on + strand at 2823.087 kb on - strand at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.164 kb on - strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.531 kb on + strand, within Pf1N1B4_2490 at 2823.532 kb on - strand, within Pf1N1B4_2490 at 2823.542 kb on + strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.543 kb on - strand, within Pf1N1B4_2490 at 2823.553 kb on + strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.554 kb on - strand, within Pf1N1B4_2490 at 2823.625 kb on + strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.626 kb on - strand, within Pf1N1B4_2490 at 2823.694 kb on + strand, within Pf1N1B4_2490 at 2823.811 kb on + strand at 2823.811 kb on + strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.851 kb on - strand at 2823.884 kb on + strand at 2823.885 kb on - strand at 2823.885 kb on - strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.907 kb on - strand, within Pf1N1B4_2492 at 2824.907 kb on - strand at 2824.970 kb on + strand at 2824.971 kb on - strand at 2824.971 kb on - strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.057 kb on + strand at 2825.057 kb on + strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.178 kb on + strand at 2825.178 kb on + strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.237 kb on + strand at 2825.237 kb on + strand at 2825.260 kb on - strand at 2825.290 kb on - strand at 2825.506 kb on + strand, within Pf1N1B4_2493 at 2825.507 kb on - strand, within Pf1N1B4_2493 at 2825.552 kb on + strand, within Pf1N1B4_2493
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,823,070 + -0.4 2,823,070 + -0.6 2,823,071 - +0.2 2,823,071 - -0.9 2,823,086 + -0.3 2,823,086 + -0.8 2,823,087 - -1.5 2,823,163 + Pf1N1B4_2490 0.11 -1.4 2,823,163 + Pf1N1B4_2490 0.11 +0.5 2,823,163 + Pf1N1B4_2490 0.11 -1.2 2,823,163 + Pf1N1B4_2490 0.11 +1.2 2,823,164 - Pf1N1B4_2490 0.11 +1.5 2,823,209 + Pf1N1B4_2490 0.16 -0.8 2,823,209 + Pf1N1B4_2490 0.16 -2.8 2,823,209 + Pf1N1B4_2490 0.16 -0.1 2,823,210 - Pf1N1B4_2490 0.16 +0.2 2,823,210 - Pf1N1B4_2490 0.16 +0.2 2,823,210 - Pf1N1B4_2490 0.16 +0.4 2,823,531 + Pf1N1B4_2490 0.56 -1.8 2,823,532 - Pf1N1B4_2490 0.56 -1.4 2,823,542 + Pf1N1B4_2490 0.57 -2.1 2,823,543 - Pf1N1B4_2490 0.57 -0.8 2,823,543 - Pf1N1B4_2490 0.57 +0.4 2,823,543 - Pf1N1B4_2490 0.57 -0.5 2,823,543 - Pf1N1B4_2490 0.57 +1.0 2,823,553 + Pf1N1B4_2490 0.59 +0.2 2,823,554 - Pf1N1B4_2490 0.59 -0.4 2,823,554 - Pf1N1B4_2490 0.59 -0.8 2,823,554 - Pf1N1B4_2490 0.59 -2.1 2,823,625 + Pf1N1B4_2490 0.68 -1.6 2,823,626 - Pf1N1B4_2490 0.68 -1.6 2,823,626 - Pf1N1B4_2490 0.68 -0.2 2,823,694 + Pf1N1B4_2490 0.76 -2.1 2,823,811 + -1.8 2,823,811 + -0.4 2,823,812 - +0.2 2,823,812 - +0.6 2,823,812 - +0.2 2,823,850 + -1.2 2,823,850 + +0.2 2,823,850 + -0.6 2,823,851 - -2.4 2,823,884 + +0.0 2,823,885 - -0.0 2,823,885 - +0.2 2,823,897 + +0.8 2,823,897 + -1.6 2,823,897 + -0.3 2,823,897 + +0.2 2,823,897 + -1.8 2,823,898 - -1.0 2,823,898 - -2.6 2,823,898 - -0.9 2,824,089 + Pf1N1B4_2491 0.30 +0.7 2,824,089 + Pf1N1B4_2491 0.30 -1.8 2,824,089 + Pf1N1B4_2491 0.30 -3.2 2,824,089 + Pf1N1B4_2491 0.30 -0.8 2,824,090 - Pf1N1B4_2491 0.30 -0.5 2,824,090 - Pf1N1B4_2491 0.30 -1.4 2,824,090 - Pf1N1B4_2491 0.30 -1.6 2,824,090 - Pf1N1B4_2491 0.30 -1.8 2,824,090 - Pf1N1B4_2491 0.30 -0.8 2,824,583 + -0.1 2,824,583 + +2.5 2,824,583 + +1.0 2,824,730 + Pf1N1B4_2492 0.15 -0.5 2,824,730 + Pf1N1B4_2492 0.15 -1.4 2,824,880 + Pf1N1B4_2492 0.65 -1.4 2,824,880 + Pf1N1B4_2492 0.65 +1.5 2,824,881 - Pf1N1B4_2492 0.66 -0.4 2,824,881 - Pf1N1B4_2492 0.66 -1.4 2,824,881 - Pf1N1B4_2492 0.66 +0.9 2,824,906 + Pf1N1B4_2492 0.74 -0.2 2,824,906 + Pf1N1B4_2492 0.74 +1.5 2,824,906 + Pf1N1B4_2492 0.74 +0.4 2,824,906 + Pf1N1B4_2492 0.74 -0.0 2,824,906 + Pf1N1B4_2492 0.74 +0.4 2,824,907 - Pf1N1B4_2492 0.74 +0.7 2,824,907 - -1.8 2,824,970 + -0.2 2,824,971 - +0.2 2,824,971 - +0.2 2,825,038 + +0.4 2,825,038 + -0.8 2,825,038 + +0.5 2,825,039 - +0.1 2,825,039 - +0.7 2,825,039 - +0.2 2,825,057 + +0.1 2,825,057 + +2.2 2,825,058 - -0.2 2,825,058 - -0.4 2,825,058 - +2.8 2,825,058 - -2.1 2,825,067 + -0.8 2,825,067 + +0.2 2,825,067 + +0.5 2,825,067 + -0.4 2,825,067 + -0.4 2,825,068 - +1.2 2,825,068 - -0.1 2,825,068 - -0.4 2,825,068 - +1.2 2,825,068 - +1.1 2,825,178 + -1.4 2,825,178 + -0.3 2,825,179 - -0.8 2,825,179 - +0.6 2,825,179 - -0.4 2,825,237 + -2.1 2,825,237 + +0.5 2,825,260 - -0.8 2,825,290 - +0.5 2,825,506 + Pf1N1B4_2493 0.14 -1.4 2,825,507 - Pf1N1B4_2493 0.14 -2.9 2,825,552 + Pf1N1B4_2493 0.16 -0.8
Or see this region's nucleotide sequence