Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2375 and Pf1N1B4_2376 are separated by 124 nucleotides Pf1N1B4_2376 and Pf1N1B4_2377 are separated by 138 nucleotides Pf1N1B4_2377 and Pf1N1B4_2378 are separated by 222 nucleotides
Pf1N1B4_2375 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 2,690,454 to 2,691,356
_2375
Pf1N1B4_2376 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 2,691,481 to 2,692,200
_2376
Pf1N1B4_2377 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,692,339 to 2,693,745
_2377
Pf1N1B4_2378 - Amine oxidase [flavin-containing] (EC 1.4.3.4), at 2,693,968 to 2,695,743
_2378
Position (kb)
2692
2693
2694 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.354 kb on + strand at 2691.355 kb on - strand at 2691.355 kb on - strand at 2692.124 kb on + strand, within Pf1N1B4_2376 at 2692.124 kb on + strand, within Pf1N1B4_2376 at 2692.125 kb on - strand, within Pf1N1B4_2376 at 2692.125 kb on - strand at 2692.213 kb on + strand at 2692.213 kb on + strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.214 kb on - strand at 2692.470 kb on - strand at 2692.470 kb on - strand at 2692.484 kb on + strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.485 kb on - strand, within Pf1N1B4_2377 at 2692.781 kb on + strand, within Pf1N1B4_2377 at 2692.781 kb on + strand, within Pf1N1B4_2377 at 2692.782 kb on - strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.826 kb on + strand, within Pf1N1B4_2377 at 2692.839 kb on - strand, within Pf1N1B4_2377 at 2692.839 kb on - strand, within Pf1N1B4_2377 at 2692.877 kb on + strand, within Pf1N1B4_2377 at 2693.036 kb on + strand, within Pf1N1B4_2377 at 2693.037 kb on - strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.048 kb on + strand, within Pf1N1B4_2377 at 2693.049 kb on - strand, within Pf1N1B4_2377 at 2693.192 kb on + strand, within Pf1N1B4_2377 at 2693.193 kb on - strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.240 kb on + strand, within Pf1N1B4_2377 at 2693.241 kb on - strand, within Pf1N1B4_2377 at 2693.251 kb on - strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.411 kb on + strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.412 kb on - strand, within Pf1N1B4_2377 at 2693.733 kb on + strand at 2693.775 kb on + strand at 2693.775 kb on + strand at 2693.801 kb on + strand at 2693.802 kb on - strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.845 kb on + strand at 2693.846 kb on - strand at 2693.846 kb on - strand at 2693.852 kb on + strand at 2693.852 kb on + strand at 2693.852 kb on + strand at 2693.853 kb on - strand at 2693.853 kb on - strand at 2693.853 kb on - strand at 2693.854 kb on + strand at 2693.855 kb on - strand at 2693.855 kb on - strand at 2693.896 kb on - strand at 2693.928 kb on + strand at 2693.929 kb on - strand at 2693.949 kb on + strand at 2693.949 kb on + strand at 2693.950 kb on - strand at 2694.042 kb on + strand at 2694.042 kb on + strand at 2694.042 kb on + strand at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.217 kb on + strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.218 kb on - strand, within Pf1N1B4_2378 at 2694.277 kb on + strand, within Pf1N1B4_2378 at 2694.278 kb on - strand, within Pf1N1B4_2378 at 2694.278 kb on - strand, within Pf1N1B4_2378 at 2694.295 kb on + strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.296 kb on - strand, within Pf1N1B4_2378 at 2694.355 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.553 kb on + strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.554 kb on - strand, within Pf1N1B4_2378 at 2694.577 kb on + strand, within Pf1N1B4_2378 at 2694.699 kb on + strand, within Pf1N1B4_2378 at 2694.745 kb on + strand, within Pf1N1B4_2378
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,691,354 + -1.0 2,691,354 + +1.2 2,691,354 + +0.3 2,691,354 + -0.1 2,691,355 - -1.1 2,691,355 - -0.7 2,692,124 + Pf1N1B4_2376 0.89 +1.3 2,692,124 + Pf1N1B4_2376 0.89 +1.3 2,692,125 - Pf1N1B4_2376 0.89 -0.7 2,692,125 - -0.3 2,692,213 + -0.5 2,692,213 + -0.3 2,692,214 - +1.3 2,692,214 - -0.7 2,692,214 - +1.5 2,692,214 - +0.3 2,692,470 - -2.5 2,692,470 - -2.1 2,692,484 + Pf1N1B4_2377 0.10 -0.5 2,692,485 - Pf1N1B4_2377 0.10 -2.3 2,692,485 - Pf1N1B4_2377 0.10 -1.3 2,692,485 - Pf1N1B4_2377 0.10 -0.4 2,692,781 + Pf1N1B4_2377 0.31 -1.2 2,692,781 + Pf1N1B4_2377 0.31 +3.7 2,692,782 - Pf1N1B4_2377 0.31 -1.3 2,692,826 + Pf1N1B4_2377 0.35 -0.0 2,692,826 + Pf1N1B4_2377 0.35 -2.1 2,692,826 + Pf1N1B4_2377 0.35 +0.3 2,692,839 - Pf1N1B4_2377 0.36 +1.6 2,692,839 - Pf1N1B4_2377 0.36 -0.7 2,692,877 + Pf1N1B4_2377 0.38 -1.3 2,693,036 + Pf1N1B4_2377 0.50 -1.7 2,693,037 - Pf1N1B4_2377 0.50 -2.9 2,693,048 + Pf1N1B4_2377 0.50 +0.3 2,693,048 + Pf1N1B4_2377 0.50 -0.5 2,693,048 + Pf1N1B4_2377 0.50 +3.1 2,693,048 + Pf1N1B4_2377 0.50 -0.9 2,693,048 + Pf1N1B4_2377 0.50 -1.9 2,693,048 + Pf1N1B4_2377 0.50 -0.7 2,693,049 - Pf1N1B4_2377 0.50 +0.8 2,693,192 + Pf1N1B4_2377 0.61 -1.3 2,693,193 - Pf1N1B4_2377 0.61 -4.0 2,693,240 + Pf1N1B4_2377 0.64 +0.7 2,693,240 + Pf1N1B4_2377 0.64 -1.7 2,693,240 + Pf1N1B4_2377 0.64 -2.1 2,693,240 + Pf1N1B4_2377 0.64 -1.5 2,693,241 - Pf1N1B4_2377 0.64 -1.3 2,693,251 - Pf1N1B4_2377 0.65 -2.1 2,693,411 + Pf1N1B4_2377 0.76 -0.7 2,693,411 + Pf1N1B4_2377 0.76 -1.5 2,693,411 + Pf1N1B4_2377 0.76 -1.3 2,693,412 - Pf1N1B4_2377 0.76 -2.6 2,693,412 - Pf1N1B4_2377 0.76 -2.5 2,693,412 - Pf1N1B4_2377 0.76 +0.8 2,693,733 + -0.7 2,693,775 + -0.3 2,693,775 + -0.5 2,693,801 + -0.3 2,693,802 - -0.6 2,693,845 + -2.7 2,693,845 + -0.1 2,693,845 + -0.7 2,693,845 + -1.3 2,693,845 + +1.0 2,693,846 - +2.3 2,693,846 - +0.8 2,693,852 + -0.5 2,693,852 + -2.1 2,693,852 + -2.3 2,693,853 - -0.3 2,693,853 - +1.3 2,693,853 - -1.3 2,693,854 + +1.1 2,693,855 - +0.9 2,693,855 - +0.3 2,693,896 - +2.1 2,693,928 + -2.1 2,693,929 - +1.1 2,693,949 + -2.1 2,693,949 + +0.3 2,693,950 - -0.2 2,694,042 + -0.9 2,694,042 + -1.7 2,694,042 + +1.3 2,694,217 + Pf1N1B4_2378 0.14 +1.3 2,694,217 + Pf1N1B4_2378 0.14 +0.7 2,694,217 + Pf1N1B4_2378 0.14 -0.7 2,694,218 - Pf1N1B4_2378 0.14 -0.3 2,694,218 - Pf1N1B4_2378 0.14 -1.3 2,694,218 - -0.7 2,694,218 - Pf1N1B4_2378 0.14 -0.1 2,694,218 - Pf1N1B4_2378 0.14 +1.7 2,694,218 - Pf1N1B4_2378 0.14 -1.3 2,694,277 + Pf1N1B4_2378 0.17 +0.1 2,694,278 - Pf1N1B4_2378 0.17 +0.3 2,694,278 - Pf1N1B4_2378 0.17 -1.3 2,694,295 + Pf1N1B4_2378 0.18 -0.1 2,694,296 - Pf1N1B4_2378 0.18 +0.1 2,694,296 - Pf1N1B4_2378 0.18 +1.3 2,694,296 - Pf1N1B4_2378 0.18 +0.4 2,694,296 - Pf1N1B4_2378 0.18 +2.1 2,694,296 - Pf1N1B4_2378 0.18 -1.1 2,694,296 - Pf1N1B4_2378 0.18 -0.1 2,694,355 + Pf1N1B4_2378 0.22 +1.3 2,694,553 + Pf1N1B4_2378 0.33 -0.2 2,694,553 + Pf1N1B4_2378 0.33 -0.3 2,694,553 + Pf1N1B4_2378 0.33 +0.8 2,694,553 + Pf1N1B4_2378 0.33 -0.4 2,694,554 - Pf1N1B4_2378 0.33 +0.5 2,694,554 - Pf1N1B4_2378 0.33 -0.7 2,694,554 - Pf1N1B4_2378 0.33 -0.4 2,694,577 + Pf1N1B4_2378 0.34 -1.4 2,694,699 + Pf1N1B4_2378 0.41 +0.3 2,694,745 + Pf1N1B4_2378 0.44 -2.9
Or see this region's nucleotide sequence