Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2353 and Pf1N1B4_2354 are separated by 57 nucleotides Pf1N1B4_2354 and Pf1N1B4_2355 are separated by 10 nucleotides Pf1N1B4_2355 and Pf1N1B4_2356 overlap by 20 nucleotides Pf1N1B4_2356 and Pf1N1B4_2357 are separated by 27 nucleotides
Pf1N1B4_2353 - twitching motility protein PilG, at 2,667,455 to 2,667,859
_2353
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Pf1N1B4_2355 - type IV pili signal transduction protein PilI, at 2,668,293 to 2,668,826
_2355
Pf1N1B4_2356 - hypothetical protein, at 2,668,807 to 2,668,929
_2356
Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014
_2357
Position (kb)
2668
2669 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2667.829 kb on - strand at 2667.857 kb on + strand at 2667.858 kb on - strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.432 kb on - strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.702 kb on + strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.703 kb on - strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.706 kb on + strand, within Pf1N1B4_2355 at 2668.707 kb on - strand, within Pf1N1B4_2355 at 2668.771 kb on + strand, within Pf1N1B4_2355 at 2668.772 kb on - strand, within Pf1N1B4_2355 at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.823 kb on - strand at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.863 kb on + strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.864 kb on - strand, within Pf1N1B4_2356 at 2668.961 kb on + strand at 2668.961 kb on + strand at 2668.962 kb on - strand at 2668.968 kb on + strand at 2669.068 kb on + strand at 2669.069 kb on - strand at 2669.101 kb on + strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.102 kb on - strand at 2669.237 kb on - strand, within Pf1N1B4_2357 at 2669.435 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.542 kb on - strand, within Pf1N1B4_2357 at 2669.794 kb on - strand, within Pf1N1B4_2357 at 2669.800 kb on - strand, within Pf1N1B4_2357 at 2669.884 kb on - strand, within Pf1N1B4_2357 at 2669.893 kb on + strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.894 kb on - strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.897 kb on + strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357 at 2669.898 kb on - strand, within Pf1N1B4_2357
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,667,829 - +0.8 2,667,857 + -0.1 2,667,858 - +1.3 2,667,925 + +2.6 2,667,925 + -0.5 2,667,925 + -1.3 2,667,925 + -0.4 2,667,926 - -1.3 2,667,926 - -0.6 2,667,926 - +0.5 2,667,926 - -0.7 2,667,926 - +0.5 2,667,926 - +0.3 2,667,926 - -1.3 2,667,926 - -0.7 2,667,926 - -0.7 2,667,926 - +0.3 2,667,926 - +1.0 2,667,926 - -1.3 2,667,959 + Pf1N1B4_2354 0.11 -1.3 2,667,959 + Pf1N1B4_2354 0.11 +1.9 2,667,959 + Pf1N1B4_2354 0.11 +0.9 2,667,959 + Pf1N1B4_2354 0.11 -2.1 2,667,959 + Pf1N1B4_2354 0.11 -1.7 2,667,960 - Pf1N1B4_2354 0.12 -2.3 2,667,960 - Pf1N1B4_2354 0.12 -0.4 2,667,960 - Pf1N1B4_2354 0.12 -0.1 2,668,160 - Pf1N1B4_2354 0.66 +1.3 2,668,217 + Pf1N1B4_2354 0.82 +0.1 2,668,217 + Pf1N1B4_2354 0.82 -1.5 2,668,217 + Pf1N1B4_2354 0.82 -1.0 2,668,217 + Pf1N1B4_2354 0.82 -1.7 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 -0.5 2,668,218 - Pf1N1B4_2354 0.82 +1.3 2,668,218 - Pf1N1B4_2354 0.82 +1.3 2,668,218 - Pf1N1B4_2354 0.82 -1.3 2,668,432 - Pf1N1B4_2355 0.26 +0.6 2,668,432 - Pf1N1B4_2355 0.26 +0.4 2,668,432 - Pf1N1B4_2355 0.26 -0.7 2,668,702 + Pf1N1B4_2355 0.77 +0.9 2,668,702 + Pf1N1B4_2355 0.77 -2.5 2,668,703 - Pf1N1B4_2355 0.77 -0.7 2,668,703 - Pf1N1B4_2355 0.77 +1.3 2,668,703 - Pf1N1B4_2355 0.77 +1.6 2,668,706 + Pf1N1B4_2355 0.77 -1.1 2,668,706 + Pf1N1B4_2355 0.77 +0.7 2,668,707 - Pf1N1B4_2355 0.78 +0.5 2,668,771 + Pf1N1B4_2355 0.90 -1.3 2,668,772 - Pf1N1B4_2355 0.90 -1.6 2,668,823 - -1.1 2,668,823 - -1.3 2,668,823 - -1.1 2,668,863 + Pf1N1B4_2356 0.46 +0.3 2,668,863 + Pf1N1B4_2356 0.46 +0.3 2,668,863 + Pf1N1B4_2356 0.46 -0.3 2,668,863 + Pf1N1B4_2356 0.46 -1.7 2,668,863 + Pf1N1B4_2356 0.46 -1.1 2,668,863 + Pf1N1B4_2356 0.46 +0.3 2,668,863 + Pf1N1B4_2356 0.46 -0.7 2,668,863 + Pf1N1B4_2356 0.46 -1.3 2,668,863 + Pf1N1B4_2356 0.46 +0.3 2,668,863 + Pf1N1B4_2356 0.46 +1.3 2,668,864 - Pf1N1B4_2356 0.46 -0.7 2,668,864 - Pf1N1B4_2356 0.46 -3.1 2,668,864 - Pf1N1B4_2356 0.46 -0.7 2,668,864 - Pf1N1B4_2356 0.46 -0.2 2,668,961 + +0.6 2,668,961 + +2.6 2,668,962 - -0.7 2,668,968 + -0.4 2,669,068 + +1.9 2,669,069 - +1.2 2,669,101 + -2.5 2,669,102 - +0.4 2,669,102 - +0.5 2,669,102 - -0.7 2,669,102 - -0.3 2,669,102 - -0.6 2,669,102 - +0.3 2,669,102 - +0.9 2,669,237 - Pf1N1B4_2357 0.14 +1.9 2,669,435 - Pf1N1B4_2357 0.23 -1.1 2,669,542 - Pf1N1B4_2357 0.28 -0.2 2,669,542 - Pf1N1B4_2357 0.28 -1.4 2,669,794 - Pf1N1B4_2357 0.41 +1.3 2,669,800 - Pf1N1B4_2357 0.41 +0.3 2,669,884 - Pf1N1B4_2357 0.45 +1.0 2,669,893 + Pf1N1B4_2357 0.45 +1.9 2,669,894 - Pf1N1B4_2357 0.46 +0.6 2,669,894 - Pf1N1B4_2357 0.46 +1.9 2,669,894 - Pf1N1B4_2357 0.46 +1.9 2,669,897 + Pf1N1B4_2357 0.46 -1.1 2,669,897 + Pf1N1B4_2357 0.46 -1.6 2,669,897 + Pf1N1B4_2357 0.46 +1.9 2,669,897 + Pf1N1B4_2357 0.46 +0.6 2,669,897 + Pf1N1B4_2357 0.46 -2.3 2,669,897 + Pf1N1B4_2357 0.46 -0.4 2,669,898 - Pf1N1B4_2357 0.46 -0.7 2,669,898 - Pf1N1B4_2357 0.46 +1.3 2,669,898 - Pf1N1B4_2357 0.46 +1.3 2,669,898 - Pf1N1B4_2357 0.46 -0.3 2,669,898 - Pf1N1B4_2357 0.46 -2.3 2,669,898 - Pf1N1B4_2357 0.46 -0.7 2,669,898 - Pf1N1B4_2357 0.46 -2.9
Or see this region's nucleotide sequence