Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2351 and Pf1N1B4_2352 are separated by 151 nucleotides Pf1N1B4_2352 and Pf1N1B4_2353 are separated by 233 nucleotides Pf1N1B4_2353 and Pf1N1B4_2354 are separated by 57 nucleotides
Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098
_2351
Pf1N1B4_2352 - Glutathione synthetase (EC 6.3.2.3), at 2,666,250 to 2,667,221
_2352
Pf1N1B4_2353 - twitching motility protein PilG, at 2,667,455 to 2,667,859
_2353
Pf1N1B4_2354 - twitching motility protein PilH, at 2,667,917 to 2,668,282
_2354
Position (kb)
2666
2667
2668 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2665.250 kb on - strand at 2665.250 kb on - strand at 2666.159 kb on + strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.160 kb on - strand at 2666.214 kb on + strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.215 kb on - strand at 2666.237 kb on + strand at 2666.238 kb on - strand at 2666.238 kb on - strand at 2666.292 kb on + strand at 2666.293 kb on - strand at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.397 kb on + strand, within Pf1N1B4_2352 at 2666.398 kb on - strand, within Pf1N1B4_2352 at 2666.621 kb on - strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.920 kb on + strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.921 kb on - strand, within Pf1N1B4_2352 at 2666.929 kb on + strand, within Pf1N1B4_2352 at 2666.930 kb on - strand, within Pf1N1B4_2352 at 2667.088 kb on + strand, within Pf1N1B4_2352 at 2667.089 kb on - strand, within Pf1N1B4_2352 at 2667.108 kb on - strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.109 kb on + strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.110 kb on - strand, within Pf1N1B4_2352 at 2667.162 kb on - strand at 2667.170 kb on + strand at 2667.170 kb on + strand at 2667.171 kb on - strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.172 kb on + strand at 2667.189 kb on - strand at 2667.328 kb on - strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.379 kb on + strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.380 kb on - strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.383 kb on + strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.384 kb on - strand at 2667.446 kb on - strand at 2667.446 kb on - strand at 2667.670 kb on - strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.709 kb on + strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.710 kb on - strand, within Pf1N1B4_2353 at 2667.829 kb on - strand at 2667.857 kb on + strand at 2667.858 kb on - strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.925 kb on + strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.926 kb on - strand at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.959 kb on + strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2667.960 kb on - strand, within Pf1N1B4_2354 at 2668.160 kb on - strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.217 kb on + strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354 at 2668.218 kb on - strand, within Pf1N1B4_2354
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,665,250 - +1.9 2,665,250 - -1.7 2,666,159 + -1.1 2,666,160 - -0.9 2,666,160 - -2.1 2,666,160 - +2.3 2,666,214 + +2.0 2,666,215 - +0.5 2,666,215 - -1.3 2,666,215 - +0.3 2,666,215 - +0.0 2,666,215 - -0.2 2,666,237 + -1.3 2,666,238 - +0.4 2,666,238 - +0.7 2,666,292 + -0.1 2,666,293 - -0.7 2,666,397 + Pf1N1B4_2352 0.15 -2.1 2,666,397 + Pf1N1B4_2352 0.15 -0.2 2,666,398 - Pf1N1B4_2352 0.15 +0.3 2,666,621 - Pf1N1B4_2352 0.38 -0.7 2,666,920 + Pf1N1B4_2352 0.69 +0.3 2,666,920 + Pf1N1B4_2352 0.69 -0.3 2,666,920 + Pf1N1B4_2352 0.69 -1.3 2,666,921 - Pf1N1B4_2352 0.69 -1.7 2,666,921 - Pf1N1B4_2352 0.69 -1.7 2,666,921 - Pf1N1B4_2352 0.69 -2.1 2,666,921 - Pf1N1B4_2352 0.69 -0.7 2,666,929 + Pf1N1B4_2352 0.70 -1.1 2,666,930 - Pf1N1B4_2352 0.70 -0.7 2,667,088 + Pf1N1B4_2352 0.86 -1.7 2,667,089 - Pf1N1B4_2352 0.86 -0.5 2,667,108 - Pf1N1B4_2352 0.88 -1.3 2,667,109 + Pf1N1B4_2352 0.88 -2.1 2,667,109 + Pf1N1B4_2352 0.88 -2.7 2,667,109 + Pf1N1B4_2352 0.88 -0.7 2,667,109 + Pf1N1B4_2352 0.88 -1.3 2,667,109 + Pf1N1B4_2352 0.88 -1.3 2,667,109 + Pf1N1B4_2352 0.88 -3.4 2,667,109 + -0.7 2,667,109 + Pf1N1B4_2352 0.88 -2.9 2,667,109 + Pf1N1B4_2352 0.88 -2.9 2,667,109 + Pf1N1B4_2352 0.88 +0.3 2,667,109 + Pf1N1B4_2352 0.88 -1.3 2,667,109 + Pf1N1B4_2352 0.88 -0.3 2,667,110 - Pf1N1B4_2352 0.88 -0.9 2,667,110 - +0.3 2,667,110 - Pf1N1B4_2352 0.88 -0.6 2,667,110 - Pf1N1B4_2352 0.88 -3.6 2,667,110 - Pf1N1B4_2352 0.88 -2.5 2,667,110 - Pf1N1B4_2352 0.88 -3.1 2,667,110 - Pf1N1B4_2352 0.88 -2.1 2,667,110 - Pf1N1B4_2352 0.88 -1.3 2,667,110 - Pf1N1B4_2352 0.88 +0.3 2,667,110 - Pf1N1B4_2352 0.88 -2.1 2,667,162 - -1.7 2,667,170 + -2.9 2,667,170 + -1.8 2,667,171 - +0.3 2,667,172 + -2.5 2,667,172 + -1.0 2,667,172 + -3.3 2,667,172 + -0.7 2,667,189 - -2.0 2,667,328 - -1.7 2,667,379 + -1.1 2,667,379 + -1.0 2,667,379 + -1.7 2,667,380 - -2.1 2,667,380 - +1.3 2,667,380 - -1.7 2,667,383 + +0.9 2,667,383 + +0.3 2,667,383 + -0.7 2,667,383 + +1.4 2,667,383 + +0.9 2,667,383 + -1.3 2,667,384 - -0.3 2,667,384 - -0.5 2,667,384 - +0.8 2,667,384 - +0.3 2,667,384 - -0.1 2,667,384 - -1.2 2,667,384 - +1.2 2,667,384 - +0.6 2,667,384 - -2.3 2,667,384 - +1.0 2,667,384 - -0.3 2,667,446 - -0.4 2,667,446 - -1.0 2,667,670 - Pf1N1B4_2353 0.53 -0.4 2,667,709 + Pf1N1B4_2353 0.63 -0.7 2,667,709 + Pf1N1B4_2353 0.63 +1.1 2,667,709 + Pf1N1B4_2353 0.63 +2.3 2,667,709 + Pf1N1B4_2353 0.63 -0.1 2,667,709 + Pf1N1B4_2353 0.63 +0.6 2,667,709 + Pf1N1B4_2353 0.63 -2.1 2,667,709 + Pf1N1B4_2353 0.63 +3.4 2,667,709 + Pf1N1B4_2353 0.63 +0.6 2,667,710 - Pf1N1B4_2353 0.63 -0.7 2,667,710 - Pf1N1B4_2353 0.63 -0.7 2,667,710 - Pf1N1B4_2353 0.63 -2.1 2,667,710 - Pf1N1B4_2353 0.63 +0.0 2,667,710 - Pf1N1B4_2353 0.63 -1.1 2,667,829 - +0.8 2,667,857 + -0.1 2,667,858 - +1.3 2,667,925 + +2.6 2,667,925 + -0.5 2,667,925 + -1.3 2,667,925 + -0.4 2,667,926 - -1.3 2,667,926 - -0.6 2,667,926 - +0.5 2,667,926 - -0.7 2,667,926 - +0.5 2,667,926 - +0.3 2,667,926 - -1.3 2,667,926 - -0.7 2,667,926 - -0.7 2,667,926 - +0.3 2,667,926 - +1.0 2,667,926 - -1.3 2,667,959 + Pf1N1B4_2354 0.11 -1.3 2,667,959 + Pf1N1B4_2354 0.11 +1.9 2,667,959 + Pf1N1B4_2354 0.11 +0.9 2,667,959 + Pf1N1B4_2354 0.11 -2.1 2,667,959 + Pf1N1B4_2354 0.11 -1.7 2,667,960 - Pf1N1B4_2354 0.12 -2.3 2,667,960 - Pf1N1B4_2354 0.12 -0.4 2,667,960 - Pf1N1B4_2354 0.12 -0.1 2,668,160 - Pf1N1B4_2354 0.66 +1.3 2,668,217 + Pf1N1B4_2354 0.82 +0.1 2,668,217 + Pf1N1B4_2354 0.82 -1.5 2,668,217 + Pf1N1B4_2354 0.82 -1.0 2,668,217 + Pf1N1B4_2354 0.82 -1.7 2,668,218 - Pf1N1B4_2354 0.82 +0.0 2,668,218 - Pf1N1B4_2354 0.82 -0.5 2,668,218 - Pf1N1B4_2354 0.82 +1.3 2,668,218 - Pf1N1B4_2354 0.82 +1.3 2,668,218 - Pf1N1B4_2354 0.82 -1.3
Or see this region's nucleotide sequence