Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides Pf1N1B4_2252 and Pf1N1B4_2253 are separated by 152 nucleotides Pf1N1B4_2253 and Pf1N1B4_2254 are separated by 227 nucleotides
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566
_2252
Pf1N1B4_2253 - Transcriptional regulator, TetR family, at 2,568,719 to 2,569,351
_2253
Pf1N1B4_2254 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 2,569,579 to 2,570,859
_2254
Position (kb)
2568
2569
2570 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2568.293 kb on - strand at 2568.314 kb on + strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.356 kb on + strand at 2568.357 kb on - strand at 2568.357 kb on - strand at 2568.378 kb on - strand, within Pf1N1B4_2252 at 2568.474 kb on - strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.520 kb on + strand, within Pf1N1B4_2252 at 2568.521 kb on - strand, within Pf1N1B4_2252 at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.564 kb on + strand at 2568.565 kb on - strand at 2568.576 kb on + strand at 2568.741 kb on + strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.765 kb on + strand at 2568.765 kb on + strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.036 kb on - strand, within Pf1N1B4_2253 at 2569.233 kb on + strand, within Pf1N1B4_2253 at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.429 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.494 kb on - strand at 2569.524 kb on + strand at 2569.524 kb on + strand at 2569.545 kb on + strand at 2569.580 kb on + strand at 2569.595 kb on + strand at 2569.596 kb on - strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.654 kb on - strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.677 kb on - strand at 2569.677 kb on - strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.787 kb on - strand, within Pf1N1B4_2254 at 2569.942 kb on - strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2570.003 kb on + strand, within Pf1N1B4_2254 at 2570.130 kb on + strand, within Pf1N1B4_2254 at 2570.220 kb on - strand, within Pf1N1B4_2254
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,568,293 - -1.7 2,568,314 + -0.8 2,568,315 - +1.2 2,568,315 - -0.8 2,568,315 - -1.0 2,568,319 + +1.0 2,568,319 + -0.5 2,568,319 + -0.2 2,568,320 - +0.5 2,568,320 - +0.2 2,568,320 - -0.2 2,568,320 - +0.1 2,568,320 - -0.9 2,568,356 + -0.2 2,568,357 - +0.7 2,568,357 - +2.4 2,568,378 - Pf1N1B4_2252 0.12 +0.1 2,568,474 - Pf1N1B4_2252 0.57 +0.9 2,568,520 + Pf1N1B4_2252 0.78 +0.4 2,568,520 + Pf1N1B4_2252 0.78 -2.7 2,568,520 + Pf1N1B4_2252 0.78 +1.2 2,568,521 - Pf1N1B4_2252 0.79 +0.7 2,568,564 + +0.2 2,568,564 + +0.2 2,568,564 + -0.8 2,568,564 + +0.2 2,568,564 + +0.6 2,568,565 - -0.0 2,568,576 + -0.6 2,568,741 + -2.6 2,568,742 - -0.2 2,568,742 - +2.0 2,568,742 - -0.8 2,568,754 - -1.3 2,568,754 - -2.2 2,568,754 - -1.0 2,568,765 + -1.0 2,568,765 + -2.1 2,568,766 - +0.1 2,568,766 - -0.2 2,568,766 - +0.2 2,568,840 + Pf1N1B4_2253 0.19 -1.3 2,568,840 + Pf1N1B4_2253 0.19 -1.0 2,568,840 + Pf1N1B4_2253 0.19 -0.6 2,568,840 + Pf1N1B4_2253 0.19 +0.2 2,568,840 + Pf1N1B4_2253 0.19 -0.6 2,568,840 + Pf1N1B4_2253 0.19 -1.3 2,568,840 + +1.2 2,568,840 + Pf1N1B4_2253 0.19 +1.5 2,568,840 + Pf1N1B4_2253 0.19 -1.3 2,568,840 + Pf1N1B4_2253 0.19 -1.8 2,568,840 + Pf1N1B4_2253 0.19 -0.8 2,568,840 + Pf1N1B4_2253 0.19 +1.8 2,568,840 + Pf1N1B4_2253 0.19 -1.3 2,568,840 + Pf1N1B4_2253 0.19 +0.2 2,568,841 - Pf1N1B4_2253 0.19 +0.8 2,568,841 - Pf1N1B4_2253 0.19 -1.3 2,568,841 - Pf1N1B4_2253 0.19 -1.3 2,568,841 - Pf1N1B4_2253 0.19 -1.8 2,568,841 - Pf1N1B4_2253 0.19 -3.2 2,568,841 - -1.3 2,568,841 - Pf1N1B4_2253 0.19 +0.7 2,568,841 - Pf1N1B4_2253 0.19 -1.3 2,568,841 - Pf1N1B4_2253 0.19 +0.2 2,569,035 + Pf1N1B4_2253 0.50 -3.1 2,569,035 + Pf1N1B4_2253 0.50 -1.3 2,569,035 + Pf1N1B4_2253 0.50 -1.3 2,569,036 - Pf1N1B4_2253 0.50 -1.1 2,569,233 + Pf1N1B4_2253 0.81 -1.8 2,569,381 + -0.4 2,569,381 + -1.8 2,569,381 + -1.1 2,569,382 - +0.6 2,569,382 - -0.5 2,569,382 - +1.8 2,569,429 + -1.3 2,569,464 + +0.2 2,569,464 + -0.8 2,569,464 + -0.8 2,569,464 + -0.8 2,569,464 + -0.8 2,569,464 + -1.1 2,569,464 + -0.2 2,569,464 + +0.8 2,569,464 + +0.2 2,569,464 + -1.3 2,569,464 + +0.2 2,569,464 + -0.5 2,569,464 + -1.5 2,569,465 - +0.2 2,569,465 - -1.8 2,569,465 - -1.3 2,569,465 - +0.2 2,569,465 - -0.5 2,569,465 - -0.6 2,569,465 - +0.8 2,569,465 - -0.8 2,569,465 - +0.2 2,569,465 - -0.3 2,569,465 - -1.0 2,569,465 - -0.0 2,569,465 - -1.0 2,569,465 - +1.7 2,569,465 - -1.8 2,569,494 - -0.6 2,569,524 + -0.8 2,569,524 + +0.8 2,569,545 + -1.1 2,569,580 + -1.2 2,569,595 + -1.7 2,569,596 - -1.0 2,569,604 + -1.3 2,569,604 + +0.4 2,569,604 + +0.5 2,569,604 + -0.2 2,569,605 - -0.8 2,569,605 - -2.3 2,569,605 - +0.2 2,569,605 - -0.6 2,569,654 - -0.4 2,569,676 + +1.6 2,569,676 + -0.2 2,569,676 + -0.3 2,569,676 + -0.1 2,569,676 + +0.3 2,569,677 - +1.5 2,569,677 - -0.5 2,569,691 + -0.8 2,569,691 + +1.5 2,569,691 + +2.2 2,569,691 + +0.4 2,569,691 + +0.2 2,569,692 - -1.8 2,569,692 - -1.8 2,569,692 - -0.2 2,569,692 - -0.0 2,569,692 - +1.6 2,569,692 - +0.2 2,569,772 + Pf1N1B4_2254 0.15 -0.8 2,569,772 + Pf1N1B4_2254 0.15 -0.8 2,569,773 - Pf1N1B4_2254 0.15 -0.1 2,569,773 - Pf1N1B4_2254 0.15 +1.0 2,569,786 + Pf1N1B4_2254 0.16 +0.2 2,569,786 + Pf1N1B4_2254 0.16 +0.7 2,569,787 - Pf1N1B4_2254 0.16 -0.7 2,569,942 - Pf1N1B4_2254 0.28 -1.4 2,569,979 + Pf1N1B4_2254 0.31 -1.0 2,569,979 + Pf1N1B4_2254 0.31 +0.8 2,569,979 + Pf1N1B4_2254 0.31 -0.2 2,569,979 + Pf1N1B4_2254 0.31 -0.7 2,569,979 + Pf1N1B4_2254 0.31 +0.2 2,569,979 + Pf1N1B4_2254 0.31 +0.2 2,569,979 + Pf1N1B4_2254 0.31 +0.2 2,569,979 + Pf1N1B4_2254 0.31 +0.2 2,569,979 + Pf1N1B4_2254 0.31 -1.6 2,569,979 + Pf1N1B4_2254 0.31 -1.3 2,569,980 - Pf1N1B4_2254 0.31 +0.2 2,569,980 - Pf1N1B4_2254 0.31 -2.8 2,569,980 - Pf1N1B4_2254 0.31 -0.8 2,569,980 - Pf1N1B4_2254 0.31 -2.6 2,569,980 - Pf1N1B4_2254 0.31 -0.6 2,569,980 - Pf1N1B4_2254 0.31 -1.3 2,570,003 + Pf1N1B4_2254 0.33 -1.8 2,570,130 + Pf1N1B4_2254 0.43 +0.7 2,570,220 - Pf1N1B4_2254 0.50 +0.6
Or see this region's nucleotide sequence