Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2236 and Pf1N1B4_2237 are separated by 163 nucleotides Pf1N1B4_2237 and Pf1N1B4_2238 are separated by 176 nucleotides
Pf1N1B4_2236 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,548,839 to 2,551,712
_2236
Pf1N1B4_2237 - Ribonucleotide reductase, alpha subunit, at 2,551,876 to 2,552,196
_2237
Pf1N1B4_2238 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,552,373 to 2,554,157
_2238
Position (kb)
2551
2552
2553 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2551.051 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.204 kb on - strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.243 kb on - strand, within Pf1N1B4_2236 at 2551.243 kb on - strand, within Pf1N1B4_2236 at 2551.350 kb on + strand, within Pf1N1B4_2236 at 2551.351 kb on - strand, within Pf1N1B4_2236 at 2551.538 kb on + strand at 2551.538 kb on + strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.575 kb on + strand at 2551.575 kb on + strand at 2551.576 kb on - strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.626 kb on + strand at 2551.626 kb on + strand at 2551.626 kb on + strand at 2551.627 kb on - strand at 2551.659 kb on + strand at 2551.659 kb on + strand at 2551.659 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.821 kb on - strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.878 kb on - strand at 2551.970 kb on - strand, within Pf1N1B4_2237 at 2551.986 kb on - strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand at 2552.103 kb on + strand, within Pf1N1B4_2237 at 2552.103 kb on + strand, within Pf1N1B4_2237 at 2552.104 kb on - strand, within Pf1N1B4_2237 at 2552.104 kb on - strand, within Pf1N1B4_2237 at 2552.173 kb on - strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.220 kb on - strand at 2552.220 kb on - strand at 2552.220 kb on - strand at 2552.243 kb on + strand at 2552.243 kb on + strand at 2552.374 kb on + strand at 2552.375 kb on - strand at 2552.375 kb on - strand at 2552.375 kb on - strand at 2552.563 kb on + strand, within Pf1N1B4_2238 at 2552.564 kb on - strand, within Pf1N1B4_2238 at 2552.564 kb on - strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,551,051 + Pf1N1B4_2236 0.77 -1.3 2,551,140 + Pf1N1B4_2236 0.80 -1.3 2,551,140 + Pf1N1B4_2236 0.80 +1.8 2,551,140 + Pf1N1B4_2236 0.80 +0.2 2,551,140 + Pf1N1B4_2236 0.80 -1.1 2,551,140 + Pf1N1B4_2236 0.80 -2.1 2,551,140 + Pf1N1B4_2236 0.80 -0.3 2,551,140 + Pf1N1B4_2236 0.80 +1.2 2,551,140 + Pf1N1B4_2236 0.80 +1.0 2,551,140 + Pf1N1B4_2236 0.80 -2.3 2,551,140 + Pf1N1B4_2236 0.80 -1.8 2,551,140 + Pf1N1B4_2236 0.80 -0.5 2,551,140 + Pf1N1B4_2236 0.80 -1.3 2,551,140 + Pf1N1B4_2236 0.80 -1.3 2,551,141 - Pf1N1B4_2236 0.80 -0.3 2,551,141 - Pf1N1B4_2236 0.80 -2.6 2,551,141 - Pf1N1B4_2236 0.80 +0.8 2,551,141 - Pf1N1B4_2236 0.80 -0.6 2,551,141 - Pf1N1B4_2236 0.80 -1.1 2,551,141 - Pf1N1B4_2236 0.80 +0.1 2,551,141 - Pf1N1B4_2236 0.80 +1.8 2,551,141 - Pf1N1B4_2236 0.80 -0.5 2,551,204 - Pf1N1B4_2236 0.82 +1.7 2,551,242 + Pf1N1B4_2236 0.84 -0.8 2,551,242 + Pf1N1B4_2236 0.84 -0.5 2,551,242 + Pf1N1B4_2236 0.84 -1.8 2,551,243 - Pf1N1B4_2236 0.84 +0.2 2,551,243 - Pf1N1B4_2236 0.84 -1.3 2,551,350 + Pf1N1B4_2236 0.87 -0.2 2,551,351 - Pf1N1B4_2236 0.87 -0.6 2,551,538 + -0.8 2,551,538 + +0.2 2,551,539 - +1.2 2,551,539 - +0.2 2,551,539 - -0.9 2,551,539 - +0.5 2,551,539 - -0.1 2,551,539 - -1.1 2,551,539 - -0.3 2,551,539 - -0.9 2,551,539 - -1.2 2,551,539 - +0.4 2,551,539 - -0.1 2,551,539 - +0.8 2,551,539 - +1.3 2,551,575 + -1.8 2,551,575 + +2.6 2,551,576 - -1.3 2,551,622 + -0.2 2,551,622 + -0.4 2,551,622 + -0.8 2,551,622 + -0.3 2,551,622 + +0.2 2,551,622 + +0.4 2,551,623 - -1.8 2,551,623 - +0.7 2,551,623 - +1.2 2,551,623 - +1.0 2,551,626 + -0.9 2,551,626 + -0.0 2,551,626 + +2.4 2,551,627 - -0.9 2,551,659 + -1.8 2,551,659 + -0.1 2,551,659 + -1.0 2,551,820 + +2.4 2,551,820 + +1.5 2,551,820 + +0.2 2,551,820 + +1.7 2,551,821 - -0.2 2,551,877 + -1.3 2,551,877 + +0.3 2,551,877 + +0.8 2,551,877 + +0.2 2,551,877 + +0.2 2,551,877 + -0.3 2,551,878 - +0.8 2,551,970 - Pf1N1B4_2237 0.29 -1.1 2,551,986 - Pf1N1B4_2237 0.34 -0.8 2,552,075 + Pf1N1B4_2237 0.62 -1.1 2,552,075 + Pf1N1B4_2237 0.62 -1.3 2,552,075 + Pf1N1B4_2237 0.62 -3.8 2,552,076 - Pf1N1B4_2237 0.62 -0.8 2,552,076 - Pf1N1B4_2237 0.62 +0.2 2,552,076 - Pf1N1B4_2237 0.62 -0.8 2,552,076 - Pf1N1B4_2237 0.62 +0.7 2,552,076 - Pf1N1B4_2237 0.62 +1.2 2,552,076 - -1.3 2,552,103 + Pf1N1B4_2237 0.71 +0.2 2,552,103 + Pf1N1B4_2237 0.71 -1.3 2,552,104 - Pf1N1B4_2237 0.71 -2.3 2,552,104 - Pf1N1B4_2237 0.71 -0.8 2,552,173 - +0.2 2,552,196 + -0.8 2,552,196 + -1.8 2,552,196 + -0.3 2,552,196 + +0.2 2,552,196 + -2.1 2,552,197 - -2.1 2,552,197 - -1.1 2,552,197 - -0.8 2,552,197 - -1.6 2,552,219 + -0.6 2,552,219 + -0.6 2,552,219 + -2.3 2,552,219 + -0.1 2,552,220 - -1.8 2,552,220 - +1.1 2,552,220 - -1.3 2,552,243 + -0.6 2,552,243 + +0.7 2,552,374 + -1.1 2,552,375 - +0.8 2,552,375 - +1.0 2,552,375 - -2.1 2,552,563 + Pf1N1B4_2238 0.11 -0.2 2,552,564 - Pf1N1B4_2238 0.11 +1.8 2,552,564 - Pf1N1B4_2238 0.11 -1.8 2,552,770 + Pf1N1B4_2238 0.22 -0.0 2,552,770 + Pf1N1B4_2238 0.22 +0.6 2,552,770 + Pf1N1B4_2238 0.22 -0.2 2,552,771 - Pf1N1B4_2238 0.22 -0.8 2,552,771 - Pf1N1B4_2238 0.22 -1.3 2,552,771 - Pf1N1B4_2238 0.22 +2.3 2,553,079 + Pf1N1B4_2238 0.40 +1.5 2,553,079 + Pf1N1B4_2238 0.40 -1.0 2,553,079 + Pf1N1B4_2238 0.40 +1.2 2,553,079 + Pf1N1B4_2238 0.40 -1.3 2,553,079 + Pf1N1B4_2238 0.40 -0.0 2,553,080 - Pf1N1B4_2238 0.40 +0.2 2,553,080 - Pf1N1B4_2238 0.40 +0.6 2,553,080 - Pf1N1B4_2238 0.40 -0.0 2,553,080 - Pf1N1B4_2238 0.40 +0.2 2,553,080 - Pf1N1B4_2238 0.40 -2.1
Or see this region's nucleotide sequence