Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_218 and Pf1N1B4_219 are separated by 144 nucleotides Pf1N1B4_219 and Pf1N1B4_220 are separated by 178 nucleotides
Pf1N1B4_218 - Periplasmic binding protein, at 277,579 to 278,322
_218
Pf1N1B4_219 - Acyclic terpenes utilization regulator AtuR, TetR family, at 278,467 to 279,093
_219
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Position (kb) 278
279
280 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 277.588 kb on - strand at 277.614 kb on - strand at 277.614 kb on - strand at 277.725 kb on - strand, within Pf1N1B4_218 at 277.725 kb on - strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.800 kb on - strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand at 277.935 kb on - strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 278.255 kb on + strand at 278.256 kb on - strand at 278.256 kb on - strand at 278.385 kb on - strand at 278.385 kb on - strand at 278.440 kb on - strand at 278.660 kb on + strand, within Pf1N1B4_219 at 278.661 kb on - strand, within Pf1N1B4_219 at 278.664 kb on + strand, within Pf1N1B4_219 at 278.720 kb on + strand, within Pf1N1B4_219 at 278.721 kb on - strand, within Pf1N1B4_219 at 278.721 kb on - strand at 278.721 kb on - strand, within Pf1N1B4_219 at 278.849 kb on + strand, within Pf1N1B4_219 at 278.914 kb on - strand, within Pf1N1B4_219 at 278.969 kb on + strand, within Pf1N1B4_219 at 279.106 kb on - strand at 279.377 kb on + strand at 279.377 kb on + strand at 279.377 kb on + strand at 279.688 kb on + strand at 279.827 kb on + strand at 279.829 kb on + strand, within Pf1N1B4_220 at 279.829 kb on + strand, within Pf1N1B4_220 at 279.830 kb on - strand, within Pf1N1B4_220 at 279.830 kb on - strand at 279.830 kb on - strand, within Pf1N1B4_220 at 279.830 kb on - strand, within Pf1N1B4_220
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 277,588 - -1.5 277,614 - +1.4 277,614 - -0.5 277,725 - Pf1N1B4_218 0.20 +1.1 277,725 - Pf1N1B4_218 0.20 +1.0 277,799 + Pf1N1B4_218 0.30 +0.0 277,799 + Pf1N1B4_218 0.30 -0.1 277,800 - Pf1N1B4_218 0.30 +0.7 277,868 + Pf1N1B4_218 0.39 +0.8 277,868 + Pf1N1B4_218 0.39 +1.4 277,869 - Pf1N1B4_218 0.39 +0.4 277,869 - Pf1N1B4_218 0.39 -0.1 277,869 - Pf1N1B4_218 0.39 -0.8 277,886 + Pf1N1B4_218 0.41 -1.4 277,886 + +1.4 277,886 + Pf1N1B4_218 0.41 -2.2 277,886 + Pf1N1B4_218 0.41 -0.2 277,887 - +1.3 277,887 - Pf1N1B4_218 0.41 +1.5 277,887 - Pf1N1B4_218 0.41 -3.0 277,887 - +1.4 277,887 - +1.1 277,902 - Pf1N1B4_218 0.43 -1.9 277,902 - Pf1N1B4_218 0.43 -0.5 277,902 - +1.4 277,935 - Pf1N1B4_218 0.48 +0.4 277,985 + Pf1N1B4_218 0.55 -0.6 277,985 + Pf1N1B4_218 0.55 +3.0 277,986 - Pf1N1B4_218 0.55 -0.7 277,986 - Pf1N1B4_218 0.55 +0.4 277,986 - Pf1N1B4_218 0.55 +2.5 278,255 + -1.8 278,256 - -0.8 278,256 - -1.8 278,385 - -0.4 278,385 - +0.2 278,440 - -1.6 278,660 + Pf1N1B4_219 0.31 -2.5 278,661 - Pf1N1B4_219 0.31 +1.1 278,664 + Pf1N1B4_219 0.31 +1.1 278,720 + Pf1N1B4_219 0.40 +1.3 278,721 - Pf1N1B4_219 0.41 +0.4 278,721 - -1.8 278,721 - Pf1N1B4_219 0.41 +0.8 278,849 + Pf1N1B4_219 0.61 +0.4 278,914 - Pf1N1B4_219 0.71 -1.5 278,969 + Pf1N1B4_219 0.80 +1.9 279,106 - -1.2 279,377 + -1.5 279,377 + +1.0 279,377 + +2.3 279,688 + -0.8 279,827 + -1.5 279,829 + Pf1N1B4_220 0.31 +0.6 279,829 + Pf1N1B4_220 0.31 -0.8 279,830 - Pf1N1B4_220 0.31 -0.2 279,830 - +0.8 279,830 - Pf1N1B4_220 0.31 -0.5 279,830 - Pf1N1B4_220 0.31 +1.8
Or see this region's nucleotide sequence