Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2188 and Pf1N1B4_2189 are separated by 352 nucleotides Pf1N1B4_2189 and Pf1N1B4_2190 are separated by 179 nucleotides Pf1N1B4_2190 and Pf1N1B4_2191 are separated by 62 nucleotides
Pf1N1B4_2188 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 2,500,225 to 2,501,463
_2188
Pf1N1B4_2189 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 2,501,816 to 2,502,367
_2189
Pf1N1B4_2190 - Regulator of sigma D, at 2,502,547 to 2,503,005
_2190
Pf1N1B4_2191 - Macrophage infectivity potentiator, at 2,503,068 to 2,503,733
_2191
Position (kb)
2501
2502
2503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2500.881 kb on - strand, within Pf1N1B4_2188 at 2501.047 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188 at 2501.382 kb on + strand at 2501.411 kb on + strand at 2501.480 kb on + strand at 2501.480 kb on + strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.481 kb on - strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.484 kb on + strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.485 kb on - strand at 2501.525 kb on - strand at 2501.562 kb on - strand at 2501.654 kb on + strand at 2501.654 kb on + strand at 2501.655 kb on - strand at 2501.655 kb on - strand at 2501.655 kb on - strand at 2501.677 kb on - strand at 2501.686 kb on + strand at 2501.686 kb on + strand at 2501.686 kb on + strand at 2501.686 kb on + strand at 2501.686 kb on + strand at 2501.686 kb on + strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.687 kb on - strand at 2501.721 kb on + strand at 2501.798 kb on + strand at 2501.798 kb on + strand at 2501.900 kb on + strand, within Pf1N1B4_2189 at 2501.900 kb on + strand, within Pf1N1B4_2189 at 2502.020 kb on + strand, within Pf1N1B4_2189 at 2502.021 kb on - strand, within Pf1N1B4_2189 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.365 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.384 kb on + strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.395 kb on + strand at 2502.395 kb on + strand at 2502.422 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.510 kb on + strand at 2502.510 kb on + strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.643 kb on + strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.789 kb on + strand, within Pf1N1B4_2190 at 2502.820 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2503.076 kb on + strand at 2503.076 kb on + strand at 2503.077 kb on - strand at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,500,881 - Pf1N1B4_2188 0.53 +0.3 2,501,047 + Pf1N1B4_2188 0.66 +0.3 2,501,122 + Pf1N1B4_2188 0.72 -0.7 2,501,122 + Pf1N1B4_2188 0.72 -1.3 2,501,382 + -0.3 2,501,411 + +0.1 2,501,480 + -0.5 2,501,480 + +0.3 2,501,481 - -1.3 2,501,481 - -0.7 2,501,481 - -0.0 2,501,481 - -1.7 2,501,481 - +0.9 2,501,481 - -0.7 2,501,481 - -0.5 2,501,481 - +0.3 2,501,481 - -2.5 2,501,481 - -0.7 2,501,481 - +0.3 2,501,481 - -1.0 2,501,481 - +0.3 2,501,481 - -0.5 2,501,484 + +0.9 2,501,484 + +0.7 2,501,484 + -2.5 2,501,484 + -1.3 2,501,484 + +0.5 2,501,484 + -1.3 2,501,484 + +0.6 2,501,484 + +0.0 2,501,485 - -0.9 2,501,485 - -1.9 2,501,485 - -1.5 2,501,485 - +0.9 2,501,485 - -2.1 2,501,485 - -1.5 2,501,485 - -2.0 2,501,485 - +0.3 2,501,485 - -3.3 2,501,485 - -0.4 2,501,525 - -0.7 2,501,562 - -0.7 2,501,654 + +0.1 2,501,654 + -1.0 2,501,655 - +0.3 2,501,655 - -0.9 2,501,655 - -0.7 2,501,677 - -2.5 2,501,686 + -0.7 2,501,686 + +1.6 2,501,686 + -3.7 2,501,686 + -0.7 2,501,686 + -1.9 2,501,686 + -0.1 2,501,687 - -1.3 2,501,687 - -1.7 2,501,687 - +0.3 2,501,687 - +0.3 2,501,687 - -1.7 2,501,687 - -1.2 2,501,687 - -2.0 2,501,721 + +2.3 2,501,798 + +0.2 2,501,798 + +1.1 2,501,900 + Pf1N1B4_2189 0.15 +0.7 2,501,900 + Pf1N1B4_2189 0.15 -2.3 2,502,020 + Pf1N1B4_2189 0.37 -3.2 2,502,021 - Pf1N1B4_2189 0.37 -0.5 2,502,148 + Pf1N1B4_2189 0.60 -0.4 2,502,148 + Pf1N1B4_2189 0.60 -2.0 2,502,149 - Pf1N1B4_2189 0.60 -0.9 2,502,149 - Pf1N1B4_2189 0.60 +0.3 2,502,149 - Pf1N1B4_2189 0.60 -1.3 2,502,365 + +1.3 2,502,375 + +0.9 2,502,375 + -0.3 2,502,375 + +0.3 2,502,384 + -1.7 2,502,385 - -0.5 2,502,385 - +0.1 2,502,385 - -0.6 2,502,395 + +1.3 2,502,395 + -0.4 2,502,422 + +1.0 2,502,450 + +1.3 2,502,450 + +0.3 2,502,450 + +0.5 2,502,450 + -1.3 2,502,451 - -2.3 2,502,451 - -0.4 2,502,451 - -0.4 2,502,451 - +0.9 2,502,451 - -1.3 2,502,451 - +1.3 2,502,451 - -0.2 2,502,451 - +0.7 2,502,451 - -1.3 2,502,510 + -0.6 2,502,510 + -0.9 2,502,511 - +0.3 2,502,511 - +0.3 2,502,511 - -2.5 2,502,511 - +1.6 2,502,643 + Pf1N1B4_2190 0.21 -0.3 2,502,643 + +1.6 2,502,643 + Pf1N1B4_2190 0.21 -1.2 2,502,644 - Pf1N1B4_2190 0.21 -2.0 2,502,644 - Pf1N1B4_2190 0.21 +1.9 2,502,644 - Pf1N1B4_2190 0.21 -1.0 2,502,724 + Pf1N1B4_2190 0.39 -1.7 2,502,724 + Pf1N1B4_2190 0.39 -0.3 2,502,724 + Pf1N1B4_2190 0.39 -1.7 2,502,725 - Pf1N1B4_2190 0.39 +1.0 2,502,725 - Pf1N1B4_2190 0.39 +0.3 2,502,751 + Pf1N1B4_2190 0.44 -0.6 2,502,751 + Pf1N1B4_2190 0.44 -0.3 2,502,751 + Pf1N1B4_2190 0.44 +0.3 2,502,751 + Pf1N1B4_2190 0.44 +0.3 2,502,752 - Pf1N1B4_2190 0.45 -0.0 2,502,752 - Pf1N1B4_2190 0.45 +1.3 2,502,752 - Pf1N1B4_2190 0.45 -2.9 2,502,789 + Pf1N1B4_2190 0.53 -0.0 2,502,820 + Pf1N1B4_2190 0.59 +1.1 2,502,869 + Pf1N1B4_2190 0.70 +0.3 2,502,869 + Pf1N1B4_2190 0.70 -2.3 2,502,869 + Pf1N1B4_2190 0.70 +0.0 2,502,869 + Pf1N1B4_2190 0.70 +0.3 2,502,869 + Pf1N1B4_2190 0.70 -1.1 2,502,869 + Pf1N1B4_2190 0.70 +1.3 2,502,869 + Pf1N1B4_2190 0.70 -0.7 2,502,869 + Pf1N1B4_2190 0.70 -0.1 2,502,869 + Pf1N1B4_2190 0.70 -1.8 2,502,869 + Pf1N1B4_2190 0.70 +0.3 2,502,869 + Pf1N1B4_2190 0.70 +0.6 2,502,869 + Pf1N1B4_2190 0.70 +0.4 2,502,869 + Pf1N1B4_2190 0.70 -1.0 2,502,869 + Pf1N1B4_2190 0.70 -1.3 2,502,870 - Pf1N1B4_2190 0.70 +0.9 2,502,870 - Pf1N1B4_2190 0.70 -1.3 2,502,870 - Pf1N1B4_2190 0.70 +1.3 2,502,870 - Pf1N1B4_2190 0.70 +0.9 2,503,076 + +0.5 2,503,076 + +0.3 2,503,077 - +0.0 2,503,151 - Pf1N1B4_2191 0.12 -1.5 2,503,151 - Pf1N1B4_2191 0.12 +1.3 2,503,235 + Pf1N1B4_2191 0.25 -0.7 2,503,235 + Pf1N1B4_2191 0.25 -2.0 2,503,235 + Pf1N1B4_2191 0.25 +0.5 2,503,236 - Pf1N1B4_2191 0.25 +0.3 2,503,236 - Pf1N1B4_2191 0.25 -2.5 2,503,303 + Pf1N1B4_2191 0.35 +0.9 2,503,303 + Pf1N1B4_2191 0.35 -0.6 2,503,303 + Pf1N1B4_2191 0.35 +0.9 2,503,303 + Pf1N1B4_2191 0.35 -1.3 2,503,303 + Pf1N1B4_2191 0.35 -1.9 2,503,303 + Pf1N1B4_2191 0.35 +0.5 2,503,303 + Pf1N1B4_2191 0.35 +0.7 2,503,303 + Pf1N1B4_2191 0.35 +1.6 2,503,303 + Pf1N1B4_2191 0.35 -0.7 2,503,303 + Pf1N1B4_2191 0.35 -0.2 2,503,303 + Pf1N1B4_2191 0.35 -1.0 2,503,303 + Pf1N1B4_2191 0.35 +1.3 2,503,303 + Pf1N1B4_2191 0.35 +2.9 2,503,303 + Pf1N1B4_2191 0.35 +0.8 2,503,304 - Pf1N1B4_2191 0.35 +1.0 2,503,304 - Pf1N1B4_2191 0.35 -1.3 2,503,304 - Pf1N1B4_2191 0.35 +0.3 2,503,304 - Pf1N1B4_2191 0.35 +0.3 2,503,304 - Pf1N1B4_2191 0.35 -0.2 2,503,304 - Pf1N1B4_2191 0.35 -2.0 2,503,304 - Pf1N1B4_2191 0.35 +0.3 2,503,304 - Pf1N1B4_2191 0.35 +0.0 2,503,304 - Pf1N1B4_2191 0.35 -0.2 2,503,304 - Pf1N1B4_2191 0.35 -0.7 2,503,304 - Pf1N1B4_2191 0.35 -1.7 2,503,304 - Pf1N1B4_2191 0.35 -2.5 2,503,304 - Pf1N1B4_2191 0.35 -0.1
Or see this region's nucleotide sequence