Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_21

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_19 and Pf1N1B4_20 are separated by 166 nucleotidesPf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotidesPf1N1B4_21 and Pf1N1B4_22 are separated by 30 nucleotidesPf1N1B4_22 and Pf1N1B4_5160 are separated by 185 nucleotidesPf1N1B4_5160 and Pf1N1B4_5161 are separated by 82 nucleotides Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485 _19 Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101 _20 Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983 _21 Pf1N1B4_22 - Transcriptional regulator, TetR family, at 25,014 to 25,553 _22 Pf1N1B4_5160 - tRNA-Glu-TTC, at 25,739 to 25,811 _5160 Pf1N1B4_5161 - tRNA-Ala-GGC, at 25,894 to 25,966 _5161 Position (kb) 24 25Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 23.349 kb on + strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.353 kb on - strand, within Pf1N1B4_19at 23.362 kb on + strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.363 kb on - strand, within Pf1N1B4_19at 23.386 kb on + strand, within Pf1N1B4_19at 23.594 kb on - strandat 23.692 kb on + strandat 23.882 kb on - strand, within Pf1N1B4_20at 23.914 kb on - strand, within Pf1N1B4_20at 24.011 kb on - strand, within Pf1N1B4_20at 24.121 kb on + strandat 24.122 kb on - strandat 24.122 kb on - strandat 24.122 kb on - strandat 24.252 kb on + strand, within Pf1N1B4_21at 24.253 kb on - strand, within Pf1N1B4_21at 24.365 kb on - strand, within Pf1N1B4_21at 24.423 kb on - strand, within Pf1N1B4_21at 24.423 kb on - strandat 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.458 kb on - strand, within Pf1N1B4_21at 24.478 kb on - strand, within Pf1N1B4_21at 24.775 kb on - strand, within Pf1N1B4_21at 25.285 kb on + strand, within Pf1N1B4_22at 25.286 kb on - strand, within Pf1N1B4_22at 25.286 kb on - strand, within Pf1N1B4_22at 25.368 kb on + strand, within Pf1N1B4_22at 25.369 kb on - strand, within Pf1N1B4_22at 25.369 kb on - strand, within Pf1N1B4_22at 25.369 kb on - strand, within Pf1N1B4_22at 25.420 kb on + strand, within Pf1N1B4_22at 25.420 kb on + strand, within Pf1N1B4_22at 25.421 kb on - strand, within Pf1N1B4_22at 25.421 kb on - strand, within Pf1N1B4_22at 25.421 kb on - strand, within Pf1N1B4_22at 25.421 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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23,349 + Pf1N1B4_19 0.86 +0.3
23,353 - Pf1N1B4_19 0.86 -2.5
23,353 - Pf1N1B4_19 0.86 -0.6
23,362 + Pf1N1B4_19 0.87 +0.6
23,363 - Pf1N1B4_19 0.87 +1.4
23,363 - Pf1N1B4_19 0.87 +0.6
23,386 + Pf1N1B4_19 0.90 -1.8
23,594 - -1.8
23,692 + -0.9
23,882 - Pf1N1B4_20 0.51 -1.2
23,914 - Pf1N1B4_20 0.58 +1.0
24,011 - Pf1N1B4_20 0.80 -2.5
24,121 + -1.2
24,122 - -0.2
24,122 - +2.8
24,122 - -0.2
24,252 + Pf1N1B4_21 0.12 -1.4
24,253 - Pf1N1B4_21 0.12 +0.1
24,365 - Pf1N1B4_21 0.26 -1.2
24,423 - Pf1N1B4_21 0.33 -3.2
24,423 - -2.2
24,458 - Pf1N1B4_21 0.37 -0.2
24,458 - Pf1N1B4_21 0.37 -1.8
24,458 - Pf1N1B4_21 0.37 -1.0
24,458 - Pf1N1B4_21 0.37 -1.8
24,478 - Pf1N1B4_21 0.39 +0.4
24,775 - Pf1N1B4_21 0.75 -0.2
25,285 + Pf1N1B4_22 0.50 -1.8
25,286 - Pf1N1B4_22 0.50 +1.6
25,286 - Pf1N1B4_22 0.50 +1.7
25,368 + Pf1N1B4_22 0.66 -0.6
25,369 - Pf1N1B4_22 0.66 -0.2
25,369 - Pf1N1B4_22 0.66 -0.0
25,369 - Pf1N1B4_22 0.66 +0.3
25,420 + Pf1N1B4_22 0.75 -1.5
25,420 + Pf1N1B4_22 0.75 +0.8
25,421 - Pf1N1B4_22 0.75 +0.8
25,421 - Pf1N1B4_22 0.75 +0.9
25,421 - Pf1N1B4_22 0.75 -1.6
25,421 - -1.2

Or see this region's nucleotide sequence