Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_19 and Pf1N1B4_20 are separated by 166 nucleotides Pf1N1B4_20 and Pf1N1B4_21 are separated by 48 nucleotides Pf1N1B4_21 and Pf1N1B4_22 are separated by 30 nucleotides Pf1N1B4_22 and Pf1N1B4_5160 are separated by 185 nucleotides Pf1N1B4_5160 and Pf1N1B4_5161 are separated by 82 nucleotides
Pf1N1B4_19 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 22,526 to 23,485
_19
Pf1N1B4_20 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 23,652 to 24,101
_20
Pf1N1B4_21 - Probable hydrolase, at 24,150 to 24,983
_21
Pf1N1B4_22 - Transcriptional regulator, TetR family, at 25,014 to 25,553
_22
Pf1N1B4_5160 - tRNA-Glu-TTC, at 25,739 to 25,811
_5160
Pf1N1B4_5161 - tRNA-Ala-GGC, at 25,894 to 25,966
_5161
Position (kb) 24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 23.349 kb on + strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.353 kb on - strand, within Pf1N1B4_19 at 23.362 kb on + strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.363 kb on - strand, within Pf1N1B4_19 at 23.386 kb on + strand, within Pf1N1B4_19 at 23.594 kb on - strand at 23.692 kb on + strand at 23.882 kb on - strand, within Pf1N1B4_20 at 23.914 kb on - strand, within Pf1N1B4_20 at 24.011 kb on - strand, within Pf1N1B4_20 at 24.121 kb on + strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.122 kb on - strand at 24.252 kb on + strand, within Pf1N1B4_21 at 24.253 kb on - strand, within Pf1N1B4_21 at 24.365 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand, within Pf1N1B4_21 at 24.423 kb on - strand at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.458 kb on - strand, within Pf1N1B4_21 at 24.478 kb on - strand, within Pf1N1B4_21 at 24.775 kb on - strand, within Pf1N1B4_21 at 25.285 kb on + strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.286 kb on - strand, within Pf1N1B4_22 at 25.368 kb on + strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.369 kb on - strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.420 kb on + strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand, within Pf1N1B4_22 at 25.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 23,349 + Pf1N1B4_19 0.86 +0.3 23,353 - Pf1N1B4_19 0.86 -2.5 23,353 - Pf1N1B4_19 0.86 -0.6 23,362 + Pf1N1B4_19 0.87 +0.6 23,363 - Pf1N1B4_19 0.87 +1.4 23,363 - Pf1N1B4_19 0.87 +0.6 23,386 + Pf1N1B4_19 0.90 -1.8 23,594 - -1.8 23,692 + -0.9 23,882 - Pf1N1B4_20 0.51 -1.2 23,914 - Pf1N1B4_20 0.58 +1.0 24,011 - Pf1N1B4_20 0.80 -2.5 24,121 + -1.2 24,122 - -0.2 24,122 - +2.8 24,122 - -0.2 24,252 + Pf1N1B4_21 0.12 -1.4 24,253 - Pf1N1B4_21 0.12 +0.1 24,365 - Pf1N1B4_21 0.26 -1.2 24,423 - Pf1N1B4_21 0.33 -3.2 24,423 - -2.2 24,458 - Pf1N1B4_21 0.37 -0.2 24,458 - Pf1N1B4_21 0.37 -1.8 24,458 - Pf1N1B4_21 0.37 -1.0 24,458 - Pf1N1B4_21 0.37 -1.8 24,478 - Pf1N1B4_21 0.39 +0.4 24,775 - Pf1N1B4_21 0.75 -0.2 25,285 + Pf1N1B4_22 0.50 -1.8 25,286 - Pf1N1B4_22 0.50 +1.6 25,286 - Pf1N1B4_22 0.50 +1.7 25,368 + Pf1N1B4_22 0.66 -0.6 25,369 - Pf1N1B4_22 0.66 -0.2 25,369 - Pf1N1B4_22 0.66 -0.0 25,369 - Pf1N1B4_22 0.66 +0.3 25,420 + Pf1N1B4_22 0.75 -1.5 25,420 + Pf1N1B4_22 0.75 +0.8 25,421 - Pf1N1B4_22 0.75 +0.8 25,421 - Pf1N1B4_22 0.75 +0.9 25,421 - Pf1N1B4_22 0.75 -1.6 25,421 - -1.2
Or see this region's nucleotide sequence