Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2020 and Pf1N1B4_2021 overlap by 21 nucleotides Pf1N1B4_2021 and Pf1N1B4_2022 overlap by 17 nucleotides
Pf1N1B4_2020 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 2,306,179 to 2,307,210
_2020
Pf1N1B4_2021 - hypothetical protein, at 2,307,190 to 2,307,489
_2021
Pf1N1B4_2022 - Transcriptional regulator, AraC family, at 2,307,473 to 2,308,471
_2022
Position (kb)
2307
2308 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2306.236 kb on + strand at 2306.236 kb on + strand at 2306.310 kb on - strand, within Pf1N1B4_2020 at 2306.358 kb on + strand, within Pf1N1B4_2020 at 2306.358 kb on + strand, within Pf1N1B4_2020 at 2306.358 kb on + strand, within Pf1N1B4_2020 at 2306.358 kb on + strand, within Pf1N1B4_2020 at 2306.359 kb on - strand, within Pf1N1B4_2020 at 2306.397 kb on - strand, within Pf1N1B4_2020 at 2306.472 kb on - strand, within Pf1N1B4_2020 at 2306.472 kb on - strand, within Pf1N1B4_2020 at 2306.472 kb on - strand, within Pf1N1B4_2020 at 2306.472 kb on - strand, within Pf1N1B4_2020 at 2306.591 kb on + strand, within Pf1N1B4_2020 at 2306.592 kb on - strand, within Pf1N1B4_2020 at 2306.592 kb on - strand, within Pf1N1B4_2020 at 2306.624 kb on + strand, within Pf1N1B4_2020 at 2306.702 kb on + strand, within Pf1N1B4_2020 at 2306.702 kb on + strand, within Pf1N1B4_2020 at 2306.702 kb on + strand, within Pf1N1B4_2020 at 2306.702 kb on + strand, within Pf1N1B4_2020 at 2306.703 kb on - strand, within Pf1N1B4_2020 at 2306.825 kb on + strand, within Pf1N1B4_2020 at 2306.826 kb on - strand, within Pf1N1B4_2020 at 2306.876 kb on + strand, within Pf1N1B4_2020 at 2306.876 kb on + strand, within Pf1N1B4_2020 at 2306.877 kb on - strand, within Pf1N1B4_2020 at 2306.877 kb on - strand, within Pf1N1B4_2020 at 2306.877 kb on - strand, within Pf1N1B4_2020 at 2306.879 kb on + strand, within Pf1N1B4_2020 at 2306.879 kb on + strand, within Pf1N1B4_2020 at 2306.879 kb on + strand, within Pf1N1B4_2020 at 2306.880 kb on - strand, within Pf1N1B4_2020 at 2306.942 kb on + strand, within Pf1N1B4_2020 at 2306.942 kb on + strand, within Pf1N1B4_2020 at 2307.012 kb on - strand, within Pf1N1B4_2020 at 2307.012 kb on - strand, within Pf1N1B4_2020 at 2307.091 kb on + strand, within Pf1N1B4_2020 at 2307.091 kb on + strand, within Pf1N1B4_2020 at 2307.091 kb on + strand, within Pf1N1B4_2020 at 2307.092 kb on - strand, within Pf1N1B4_2020 at 2307.197 kb on + strand at 2307.197 kb on + strand at 2307.197 kb on + strand at 2307.198 kb on - strand at 2307.198 kb on - strand at 2307.435 kb on + strand, within Pf1N1B4_2021 at 2307.435 kb on + strand, within Pf1N1B4_2021 at 2307.436 kb on - strand, within Pf1N1B4_2021 at 2307.436 kb on - strand, within Pf1N1B4_2021 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.649 kb on - strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.755 kb on + strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2307.756 kb on - strand, within Pf1N1B4_2022 at 2308.158 kb on - strand at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.196 kb on + strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.197 kb on - strand, within Pf1N1B4_2022 at 2308.214 kb on + strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.215 kb on - strand, within Pf1N1B4_2022 at 2308.237 kb on - strand, within Pf1N1B4_2022 at 2308.286 kb on + strand at 2308.286 kb on + strand, within Pf1N1B4_2022 at 2308.287 kb on - strand, within Pf1N1B4_2022 at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.488 kb on + strand at 2308.489 kb on - strand at 2308.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,306,236 + -2.9 2,306,236 + +1.4 2,306,310 - Pf1N1B4_2020 0.13 +0.1 2,306,358 + Pf1N1B4_2020 0.17 +1.4 2,306,358 + Pf1N1B4_2020 0.17 +0.5 2,306,358 + Pf1N1B4_2020 0.17 -1.2 2,306,358 + Pf1N1B4_2020 0.17 -1.9 2,306,359 - Pf1N1B4_2020 0.17 -1.0 2,306,397 - Pf1N1B4_2020 0.21 -0.2 2,306,472 - Pf1N1B4_2020 0.28 +0.8 2,306,472 - Pf1N1B4_2020 0.28 +0.3 2,306,472 - Pf1N1B4_2020 0.28 -0.8 2,306,472 - Pf1N1B4_2020 0.28 -0.0 2,306,591 + Pf1N1B4_2020 0.40 +1.7 2,306,592 - Pf1N1B4_2020 0.40 +0.4 2,306,592 - Pf1N1B4_2020 0.40 +0.4 2,306,624 + Pf1N1B4_2020 0.43 +0.2 2,306,702 + Pf1N1B4_2020 0.51 -1.9 2,306,702 + Pf1N1B4_2020 0.51 +0.1 2,306,702 + Pf1N1B4_2020 0.51 -1.3 2,306,702 + Pf1N1B4_2020 0.51 +3.2 2,306,703 - Pf1N1B4_2020 0.51 -0.6 2,306,825 + Pf1N1B4_2020 0.63 +0.9 2,306,826 - Pf1N1B4_2020 0.63 +0.2 2,306,876 + Pf1N1B4_2020 0.68 -1.6 2,306,876 + Pf1N1B4_2020 0.68 -0.2 2,306,877 - Pf1N1B4_2020 0.68 -0.3 2,306,877 - Pf1N1B4_2020 0.68 +1.0 2,306,877 - Pf1N1B4_2020 0.68 +0.1 2,306,879 + Pf1N1B4_2020 0.68 +1.2 2,306,879 + Pf1N1B4_2020 0.68 -1.8 2,306,879 + Pf1N1B4_2020 0.68 +0.4 2,306,880 - Pf1N1B4_2020 0.68 +1.4 2,306,942 + Pf1N1B4_2020 0.74 -2.2 2,306,942 + Pf1N1B4_2020 0.74 -1.9 2,307,012 - Pf1N1B4_2020 0.81 -0.6 2,307,012 - Pf1N1B4_2020 0.81 -1.6 2,307,091 + Pf1N1B4_2020 0.88 -2.1 2,307,091 + Pf1N1B4_2020 0.88 -0.9 2,307,091 + Pf1N1B4_2020 0.88 +2.4 2,307,092 - Pf1N1B4_2020 0.88 +0.4 2,307,197 + +0.4 2,307,197 + +2.5 2,307,197 + +0.2 2,307,198 - +0.4 2,307,198 - +2.4 2,307,435 + Pf1N1B4_2021 0.82 -1.2 2,307,435 + Pf1N1B4_2021 0.82 -0.2 2,307,436 - Pf1N1B4_2021 0.82 -2.4 2,307,436 - Pf1N1B4_2021 0.82 -0.2 2,307,649 - Pf1N1B4_2022 0.18 -0.6 2,307,649 - Pf1N1B4_2022 0.18 -0.9 2,307,649 - Pf1N1B4_2022 0.18 +2.0 2,307,755 + Pf1N1B4_2022 0.28 +1.0 2,307,755 + Pf1N1B4_2022 0.28 +1.4 2,307,755 + Pf1N1B4_2022 0.28 +1.7 2,307,756 - Pf1N1B4_2022 0.28 -0.3 2,307,756 - Pf1N1B4_2022 0.28 +1.8 2,307,756 - Pf1N1B4_2022 0.28 -1.0 2,307,756 - Pf1N1B4_2022 0.28 +2.6 2,308,158 - +0.8 2,308,196 + Pf1N1B4_2022 0.72 +0.4 2,308,196 + Pf1N1B4_2022 0.72 +0.1 2,308,196 + Pf1N1B4_2022 0.72 -1.6 2,308,196 + Pf1N1B4_2022 0.72 +0.8 2,308,197 - Pf1N1B4_2022 0.72 -0.6 2,308,197 - Pf1N1B4_2022 0.72 -2.2 2,308,197 - Pf1N1B4_2022 0.72 +0.6 2,308,214 + Pf1N1B4_2022 0.74 -0.5 2,308,215 - Pf1N1B4_2022 0.74 +0.7 2,308,215 - Pf1N1B4_2022 0.74 +2.0 2,308,237 - Pf1N1B4_2022 0.76 +0.8 2,308,286 + +0.4 2,308,286 + Pf1N1B4_2022 0.81 -1.9 2,308,287 - Pf1N1B4_2022 0.81 -0.1 2,308,488 + +0.0 2,308,488 + +0.1 2,308,488 + +1.4 2,308,489 - -0.0 2,308,489 - +1.1
Or see this region's nucleotide sequence