Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1934 and Pf1N1B4_1935 overlap by 4 nucleotides Pf1N1B4_1935 and Pf1N1B4_1936 are separated by 18 nucleotides Pf1N1B4_1936 and Pf1N1B4_1937 are separated by 9 nucleotides
Pf1N1B4_1934 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 2,213,442 to 2,215,340
_1934
Pf1N1B4_1935 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 2,215,337 to 2,215,981
_1935
Pf1N1B4_1936 - Chromosome (plasmid) partitioning protein ParA, at 2,216,000 to 2,216,797
_1936
Pf1N1B4_1937 - Chromosome (plasmid) partitioning protein ParB, at 2,216,807 to 2,217,679
_1937
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2216.010 kb on + strand at 2216.010 kb on + strand at 2216.010 kb on + strand at 2216.011 kb on - strand at 2216.011 kb on - strand at 2216.011 kb on - strand at 2216.060 kb on - strand at 2216.060 kb on - strand at 2216.225 kb on + strand, within Pf1N1B4_1936 at 2216.226 kb on - strand, within Pf1N1B4_1936 at 2216.226 kb on - strand, within Pf1N1B4_1936 at 2216.322 kb on + strand, within Pf1N1B4_1936 at 2216.354 kb on + strand, within Pf1N1B4_1936 at 2216.355 kb on - strand, within Pf1N1B4_1936 at 2216.360 kb on + strand, within Pf1N1B4_1936 at 2216.453 kb on + strand, within Pf1N1B4_1936 at 2216.453 kb on + strand, within Pf1N1B4_1936 at 2216.555 kb on + strand, within Pf1N1B4_1936 at 2216.555 kb on + strand, within Pf1N1B4_1936 at 2216.555 kb on + strand, within Pf1N1B4_1936 at 2216.555 kb on + strand, within Pf1N1B4_1936 at 2216.555 kb on + strand, within Pf1N1B4_1936 at 2216.556 kb on - strand, within Pf1N1B4_1936 at 2216.556 kb on - strand, within Pf1N1B4_1936 at 2216.556 kb on - strand, within Pf1N1B4_1936 at 2216.556 kb on - strand, within Pf1N1B4_1936 at 2216.624 kb on + strand, within Pf1N1B4_1936 at 2216.672 kb on + strand, within Pf1N1B4_1936 at 2216.672 kb on + strand, within Pf1N1B4_1936 at 2216.672 kb on + strand, within Pf1N1B4_1936 at 2216.723 kb on + strand at 2216.795 kb on + strand at 2216.795 kb on + strand at 2216.795 kb on + strand at 2216.893 kb on - strand at 2216.893 kb on - strand at 2216.951 kb on + strand, within Pf1N1B4_1937 at 2216.952 kb on - strand, within Pf1N1B4_1937 at 2217.610 kb on + strand at 2217.621 kb on + strand at 2217.621 kb on + strand at 2217.621 kb on + strand at 2217.694 kb on + strand at 2217.694 kb on + strand at 2217.694 kb on + strand at 2217.694 kb on + strand at 2217.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 2,216,010 + -2.9 2,216,010 + +1.7 2,216,010 + -0.8 2,216,011 - -1.2 2,216,011 - +2.2 2,216,011 - +1.0 2,216,060 - -0.9 2,216,060 - +0.7 2,216,225 + Pf1N1B4_1936 0.28 -2.4 2,216,226 - Pf1N1B4_1936 0.28 +0.8 2,216,226 - Pf1N1B4_1936 0.28 -1.9 2,216,322 + Pf1N1B4_1936 0.40 +0.4 2,216,354 + Pf1N1B4_1936 0.44 -1.6 2,216,355 - Pf1N1B4_1936 0.44 -1.2 2,216,360 + Pf1N1B4_1936 0.45 +0.1 2,216,453 + Pf1N1B4_1936 0.57 +0.4 2,216,453 + Pf1N1B4_1936 0.57 -1.1 2,216,555 + Pf1N1B4_1936 0.70 -0.6 2,216,555 + Pf1N1B4_1936 0.70 +1.0 2,216,555 + Pf1N1B4_1936 0.70 -0.6 2,216,555 + Pf1N1B4_1936 0.70 -2.6 2,216,555 + Pf1N1B4_1936 0.70 -2.4 2,216,556 - Pf1N1B4_1936 0.70 -2.4 2,216,556 - Pf1N1B4_1936 0.70 +1.4 2,216,556 - Pf1N1B4_1936 0.70 +2.4 2,216,556 - Pf1N1B4_1936 0.70 -1.6 2,216,624 + Pf1N1B4_1936 0.78 -1.2 2,216,672 + Pf1N1B4_1936 0.84 +0.4 2,216,672 + Pf1N1B4_1936 0.84 -2.6 2,216,672 + Pf1N1B4_1936 0.84 -0.6 2,216,723 + -0.6 2,216,795 + -0.2 2,216,795 + -0.6 2,216,795 + -1.2 2,216,893 - -1.6 2,216,893 - +1.4 2,216,951 + Pf1N1B4_1937 0.16 -1.2 2,216,952 - Pf1N1B4_1937 0.17 -0.8 2,217,610 + +1.0 2,217,621 + +1.4 2,217,621 + +0.4 2,217,621 + -1.9 2,217,694 + -0.9 2,217,694 + +0.8 2,217,694 + +1.5 2,217,694 + +0.4 2,217,730 + +0.4
Or see this region's nucleotide sequence