Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1875

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1873 and Pf1N1B4_1874 are separated by 59 nucleotidesPf1N1B4_1874 and Pf1N1B4_1875 are separated by 74 nucleotidesPf1N1B4_1875 and Pf1N1B4_1876 are separated by 300 nucleotides Pf1N1B4_1873 - Homoserine kinase (EC 2.7.1.39), at 2,142,647 to 2,143,597 _1873 Pf1N1B4_1874 - FIG00953549: hypothetical protein, at 2,143,657 to 2,143,944 _1874 Pf1N1B4_1875 - DNA polymerase I (EC 2.7.7.7), at 2,144,019 to 2,146,787 _1875 Pf1N1B4_1876 - GTP-binding protein EngB, at 2,147,088 to 2,147,729 _1876 Position (kb) 2144 2145 2146 2147Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2143.643 kb on - strandat 2143.643 kb on - strandat 2143.648 kb on - strandat 2143.648 kb on - strandat 2143.648 kb on - strandat 2143.648 kb on - strandat 2143.648 kb on - strandat 2143.659 kb on - strandat 2143.670 kb on - strandat 2143.752 kb on - strand, within Pf1N1B4_1874at 2143.752 kb on - strand, within Pf1N1B4_1874at 2143.752 kb on - strand, within Pf1N1B4_1874at 2143.752 kb on - strandat 2143.788 kb on - strand, within Pf1N1B4_1874at 2143.846 kb on + strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strandat 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.847 kb on - strand, within Pf1N1B4_1874at 2143.933 kb on + strandat 2143.934 kb on - strandat 2143.947 kb on + strandat 2146.818 kb on + strandat 2146.846 kb on + strandat 2146.846 kb on + strandat 2146.847 kb on - strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.848 kb on + strandat 2146.849 kb on - strandat 2146.870 kb on + strandat 2146.871 kb on - strandat 2146.892 kb on + strandat 2146.902 kb on + strandat 2146.962 kb on - strandat 2146.972 kb on - strandat 2147.081 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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2,143,643 - +0.7
2,143,643 - -0.3
2,143,648 - -0.4
2,143,648 - +0.8
2,143,648 - +0.2
2,143,648 - +0.4
2,143,648 - -0.6
2,143,659 - +2.7
2,143,670 - -1.4
2,143,752 - Pf1N1B4_1874 0.33 +0.4
2,143,752 - Pf1N1B4_1874 0.33 -2.2
2,143,752 - Pf1N1B4_1874 0.33 -2.2
2,143,752 - -0.6
2,143,788 - Pf1N1B4_1874 0.45 +0.1
2,143,846 + Pf1N1B4_1874 0.66 +0.1
2,143,847 - Pf1N1B4_1874 0.66 -2.8
2,143,847 - Pf1N1B4_1874 0.66 -0.6
2,143,847 - Pf1N1B4_1874 0.66 -1.6
2,143,847 - Pf1N1B4_1874 0.66 -0.6
2,143,847 - -0.6
2,143,847 - Pf1N1B4_1874 0.66 +0.4
2,143,847 - Pf1N1B4_1874 0.66 -0.9
2,143,847 - Pf1N1B4_1874 0.66 +0.0
2,143,847 - Pf1N1B4_1874 0.66 +0.4
2,143,933 + +1.4
2,143,934 - -0.3
2,143,947 + -0.6
2,146,818 + +0.8
2,146,846 + -0.6
2,146,846 + +0.1
2,146,847 - -2.2
2,146,848 + +0.7
2,146,848 + +0.4
2,146,848 + -0.2
2,146,848 + -2.4
2,146,848 + -1.2
2,146,848 + -0.6
2,146,848 + +0.0
2,146,849 - +1.0
2,146,870 + +0.4
2,146,871 - -1.9
2,146,892 + +1.4
2,146,902 + -0.0
2,146,962 - +0.4
2,146,972 - -1.2
2,147,081 + -1.9

Or see this region's nucleotide sequence