Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_184 and Pf1N1B4_185 are separated by 11 nucleotides Pf1N1B4_185 and Pf1N1B4_186 overlap by 30 nucleotides Pf1N1B4_186 and Pf1N1B4_5165 are separated by 90 nucleotides Pf1N1B4_5165 and Pf1N1B4_5166 are separated by 169 nucleotides
Pf1N1B4_184 - Chromosome initiation inhibitor, at 227,441 to 228,400
_184
Pf1N1B4_185 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 228,412 to 230,304
_185
Pf1N1B4_186 - hypothetical protein, at 230,275 to 230,457
_186
Pf1N1B4_5165 - 5S RNA, at 230,548 to 230,667
_5165
Pf1N1B4_5166 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 230,837 to 233,729
_5166
Position (kb)
228
229
230
231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 227.412 kb on + strand at 227.427 kb on + strand at 227.440 kb on + strand at 227.456 kb on - strand at 227.516 kb on + strand at 227.516 kb on + strand at 227.517 kb on - strand at 227.517 kb on - strand at 227.807 kb on + strand, within Pf1N1B4_184 at 227.967 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.984 kb on + strand, within Pf1N1B4_184 at 227.985 kb on - strand, within Pf1N1B4_184 at 227.991 kb on - strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.179 kb on + strand, within Pf1N1B4_184 at 228.231 kb on + strand, within Pf1N1B4_184 at 228.306 kb on + strand at 228.306 kb on + strand at 228.306 kb on + strand at 228.306 kb on + strand at 228.307 kb on - strand at 228.379 kb on + strand at 228.414 kb on - strand at 228.452 kb on + strand at 228.453 kb on - strand at 228.647 kb on + strand, within Pf1N1B4_185 at 228.648 kb on - strand, within Pf1N1B4_185 at 228.757 kb on + strand, within Pf1N1B4_185 at 228.757 kb on + strand, within Pf1N1B4_185 at 228.758 kb on - strand, within Pf1N1B4_185 at 228.808 kb on + strand, within Pf1N1B4_185 at 228.809 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 228.945 kb on - strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.094 kb on + strand, within Pf1N1B4_185 at 229.095 kb on - strand, within Pf1N1B4_185 at 229.137 kb on - strand, within Pf1N1B4_185 at 229.137 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.143 kb on - strand, within Pf1N1B4_185 at 229.214 kb on + strand at 229.214 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.218 kb on + strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.219 kb on - strand, within Pf1N1B4_185 at 229.241 kb on + strand, within Pf1N1B4_185 at 229.398 kb on - strand, within Pf1N1B4_185 at 229.398 kb on - strand, within Pf1N1B4_185 at 229.673 kb on + strand, within Pf1N1B4_185 at 229.673 kb on + strand at 229.718 kb on + strand, within Pf1N1B4_185 at 229.719 kb on - strand, within Pf1N1B4_185 at 229.896 kb on - strand, within Pf1N1B4_185 at 230.090 kb on + strand, within Pf1N1B4_185 at 230.122 kb on + strand at 230.122 kb on + strand at 230.122 kb on + strand at 230.123 kb on - strand at 230.159 kb on + strand at 230.159 kb on + strand at 230.159 kb on + strand at 230.165 kb on + strand at 230.186 kb on + strand at 230.186 kb on + strand at 230.186 kb on + strand at 230.186 kb on + strand at 230.186 kb on + strand at 230.187 kb on - strand at 230.187 kb on - strand at 230.187 kb on - strand at 230.214 kb on + strand at 230.251 kb on - strand at 230.327 kb on + strand, within Pf1N1B4_186 at 230.327 kb on + strand, within Pf1N1B4_186 at 230.328 kb on - strand, within Pf1N1B4_186 at 230.443 kb on + strand at 230.443 kb on + strand at 230.444 kb on - strand at 230.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 227,412 + +1.0 227,427 + -0.0 227,440 + -0.2 227,456 - -2.5 227,516 + -0.1 227,516 + +2.1 227,517 - +1.4 227,517 - +0.4 227,807 + Pf1N1B4_184 0.38 +0.2 227,967 + Pf1N1B4_184 0.55 -0.8 227,984 + Pf1N1B4_184 0.57 +1.6 227,984 + Pf1N1B4_184 0.57 -0.2 227,984 + Pf1N1B4_184 0.57 -0.5 227,984 + Pf1N1B4_184 0.57 +0.1 227,985 - Pf1N1B4_184 0.57 -0.8 227,991 - Pf1N1B4_184 0.57 +0.2 228,179 + Pf1N1B4_184 0.77 +0.4 228,179 + Pf1N1B4_184 0.77 +0.8 228,231 + Pf1N1B4_184 0.82 -1.8 228,306 + -0.2 228,306 + -0.2 228,306 + +0.1 228,306 + -0.2 228,307 - +2.0 228,379 + -0.4 228,414 - -0.5 228,452 + -0.2 228,453 - +1.3 228,647 + Pf1N1B4_185 0.12 -2.2 228,648 - Pf1N1B4_185 0.12 -0.8 228,757 + Pf1N1B4_185 0.18 -0.2 228,757 + Pf1N1B4_185 0.18 +1.6 228,758 - Pf1N1B4_185 0.18 -0.6 228,808 + Pf1N1B4_185 0.21 -0.7 228,809 - Pf1N1B4_185 0.21 +0.1 228,945 - Pf1N1B4_185 0.28 -0.2 228,945 - Pf1N1B4_185 0.28 +1.1 228,945 - Pf1N1B4_185 0.28 +2.1 229,094 + Pf1N1B4_185 0.36 +0.7 229,094 + Pf1N1B4_185 0.36 +2.3 229,094 + Pf1N1B4_185 0.36 +1.2 229,094 + Pf1N1B4_185 0.36 -1.3 229,095 - Pf1N1B4_185 0.36 -0.4 229,137 - Pf1N1B4_185 0.38 -1.8 229,137 - Pf1N1B4_185 0.38 -1.2 229,143 - Pf1N1B4_185 0.39 +1.2 229,143 - Pf1N1B4_185 0.39 +1.4 229,143 - Pf1N1B4_185 0.39 -0.2 229,143 - Pf1N1B4_185 0.39 -2.7 229,143 - Pf1N1B4_185 0.39 -1.1 229,143 - Pf1N1B4_185 0.39 -1.2 229,143 - Pf1N1B4_185 0.39 -1.0 229,143 - Pf1N1B4_185 0.39 -1.5 229,143 - Pf1N1B4_185 0.39 -2.2 229,143 - Pf1N1B4_185 0.39 -1.2 229,214 + +0.4 229,214 + Pf1N1B4_185 0.42 +0.4 229,218 + Pf1N1B4_185 0.43 +0.2 229,218 + Pf1N1B4_185 0.43 -1.3 229,218 + Pf1N1B4_185 0.43 -0.8 229,219 - Pf1N1B4_185 0.43 +0.4 229,219 - Pf1N1B4_185 0.43 -1.2 229,219 - Pf1N1B4_185 0.43 +0.4 229,219 - Pf1N1B4_185 0.43 -1.3 229,219 - Pf1N1B4_185 0.43 +2.1 229,241 + Pf1N1B4_185 0.44 +0.5 229,398 - Pf1N1B4_185 0.52 -0.2 229,398 - Pf1N1B4_185 0.52 +1.1 229,673 + Pf1N1B4_185 0.67 -2.2 229,673 + -1.8 229,718 + Pf1N1B4_185 0.69 -0.8 229,719 - Pf1N1B4_185 0.69 +0.6 229,896 - Pf1N1B4_185 0.78 +1.0 230,090 + Pf1N1B4_185 0.89 -0.6 230,122 + +1.2 230,122 + -0.5 230,122 + +1.3 230,123 - -1.2 230,159 + +0.1 230,159 + +0.6 230,159 + +1.0 230,165 + -0.2 230,186 + -3.4 230,186 + +1.1 230,186 + +0.4 230,186 + +0.6 230,186 + -0.0 230,187 - +0.3 230,187 - -0.2 230,187 - -2.5 230,214 + -0.2 230,251 - -1.8 230,327 + Pf1N1B4_186 0.28 -1.2 230,327 + Pf1N1B4_186 0.28 +1.1 230,328 - Pf1N1B4_186 0.29 -0.6 230,443 + -0.2 230,443 + +1.1 230,444 - -0.6 230,444 - -0.2
Or see this region's nucleotide sequence