Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1623 and Pf1N1B4_1624 are separated by 9 nucleotides Pf1N1B4_1624 and Pf1N1B4_1625 are separated by 99 nucleotides Pf1N1B4_1625 and Pf1N1B4_1626 are separated by 0 nucleotides Pf1N1B4_1626 and Pf1N1B4_1627 overlap by 1 nucleotides
Pf1N1B4_1623 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 1,867,729 to 1,868,499
_1623
Pf1N1B4_1624 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 1,868,509 to 1,869,246
_1624
Pf1N1B4_1625 - FIG00956267: hypothetical protein, at 1,869,346 to 1,869,603
_1625
Pf1N1B4_1626 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 1,869,604 to 1,870,242
_1626
Pf1N1B4_1627 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 1,870,242 to 1,870,877
_1627
Position (kb)
1869
1870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1868.355 kb on + strand, within Pf1N1B4_1623 at 1868.519 kb on + strand at 1868.519 kb on + strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.702 kb on + strand, within Pf1N1B4_1624 at 1868.703 kb on - strand, within Pf1N1B4_1624 at 1868.703 kb on - strand, within Pf1N1B4_1624 at 1868.749 kb on - strand, within Pf1N1B4_1624 at 1868.750 kb on + strand, within Pf1N1B4_1624 at 1868.751 kb on - strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.969 kb on + strand, within Pf1N1B4_1624 at 1868.969 kb on + strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.219 kb on + strand at 1869.219 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.238 kb on - strand at 1869.238 kb on - strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.283 kb on - strand at 1869.283 kb on - strand at 1869.304 kb on + strand at 1869.347 kb on + strand at 1869.348 kb on - strand at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.437 kb on + strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.606 kb on - strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1870.466 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,868,355 + Pf1N1B4_1623 0.81 -1.3 1,868,519 + +0.3 1,868,519 + -1.3 1,868,520 - -2.5 1,868,520 - -0.7 1,868,520 - -0.8 1,868,520 - +0.3 1,868,567 + -2.3 1,868,567 + -0.4 1,868,567 + -3.3 1,868,567 + -1.7 1,868,568 - -0.3 1,868,568 - -0.7 1,868,568 - -0.7 1,868,568 - +0.3 1,868,568 - -2.3 1,868,568 - -2.0 1,868,568 - -0.1 1,868,702 + Pf1N1B4_1624 0.26 -1.3 1,868,703 - Pf1N1B4_1624 0.26 -2.0 1,868,703 - Pf1N1B4_1624 0.26 -1.5 1,868,749 - Pf1N1B4_1624 0.33 -0.7 1,868,750 + Pf1N1B4_1624 0.33 -0.7 1,868,751 - Pf1N1B4_1624 0.33 -0.7 1,868,948 + Pf1N1B4_1624 0.59 -2.6 1,868,948 + Pf1N1B4_1624 0.59 -2.5 1,868,948 + Pf1N1B4_1624 0.59 -2.7 1,868,948 + Pf1N1B4_1624 0.59 -2.0 1,868,948 + Pf1N1B4_1624 0.59 -2.0 1,868,949 - Pf1N1B4_1624 0.60 -2.3 1,868,949 - Pf1N1B4_1624 0.60 -0.7 1,868,949 - Pf1N1B4_1624 0.60 -1.0 1,868,969 + Pf1N1B4_1624 0.62 -0.2 1,868,969 + Pf1N1B4_1624 0.62 -3.1 1,868,970 - Pf1N1B4_1624 0.62 -2.1 1,868,970 - Pf1N1B4_1624 0.62 -1.7 1,868,970 - Pf1N1B4_1624 0.62 -2.3 1,868,970 - Pf1N1B4_1624 0.62 +1.3 1,869,052 + Pf1N1B4_1624 0.74 -1.3 1,869,052 + Pf1N1B4_1624 0.74 -1.1 1,869,052 + Pf1N1B4_1624 0.74 -1.3 1,869,052 + Pf1N1B4_1624 0.74 -2.0 1,869,052 + Pf1N1B4_1624 0.74 +0.8 1,869,053 - Pf1N1B4_1624 0.74 -1.8 1,869,053 - Pf1N1B4_1624 0.74 -1.3 1,869,053 - Pf1N1B4_1624 0.74 -2.7 1,869,219 + +0.3 1,869,219 + -1.0 1,869,237 + +0.7 1,869,237 + -1.7 1,869,237 + +0.7 1,869,237 + -2.0 1,869,238 - -1.3 1,869,238 - -2.5 1,869,241 + -0.7 1,869,241 + +0.3 1,869,241 + -2.0 1,869,241 + -2.8 1,869,241 + -1.7 1,869,241 + -1.5 1,869,241 + -2.0 1,869,241 + -0.4 1,869,242 - -0.7 1,869,242 - +1.3 1,869,242 - -1.8 1,869,242 - -0.7 1,869,242 - -1.3 1,869,242 - -1.5 1,869,282 + -2.0 1,869,282 + -1.6 1,869,282 + -1.4 1,869,282 + -0.2 1,869,282 + -1.3 1,869,282 + -1.1 1,869,282 + -1.0 1,869,282 + -0.7 1,869,282 + +0.3 1,869,282 + -0.4 1,869,282 + -1.7 1,869,282 + +0.2 1,869,282 + +0.3 1,869,283 - -0.3 1,869,283 - +0.0 1,869,304 + -1.3 1,869,347 + -3.3 1,869,348 - -1.0 1,869,434 + Pf1N1B4_1625 0.34 +0.3 1,869,434 + Pf1N1B4_1625 0.34 -1.3 1,869,434 + Pf1N1B4_1625 0.34 -2.6 1,869,434 + Pf1N1B4_1625 0.34 -2.8 1,869,434 + Pf1N1B4_1625 0.34 -1.1 1,869,434 + Pf1N1B4_1625 0.34 -1.7 1,869,434 + Pf1N1B4_1625 0.34 +1.1 1,869,434 + Pf1N1B4_1625 0.34 -2.3 1,869,435 - Pf1N1B4_1625 0.34 -1.3 1,869,435 - Pf1N1B4_1625 0.34 +1.9 1,869,435 - Pf1N1B4_1625 0.34 +0.3 1,869,437 + Pf1N1B4_1625 0.35 -0.7 1,869,438 - Pf1N1B4_1625 0.36 -0.7 1,869,438 - Pf1N1B4_1625 0.36 -1.3 1,869,606 - -0.1 1,869,776 + Pf1N1B4_1626 0.27 -1.3 1,869,776 + Pf1N1B4_1626 0.27 -0.1 1,869,776 + -1.3 1,869,776 + Pf1N1B4_1626 0.27 -2.7 1,869,776 + Pf1N1B4_1626 0.27 -1.3 1,869,776 + Pf1N1B4_1626 0.27 -1.5 1,869,776 + Pf1N1B4_1626 0.27 -2.0 1,869,776 + Pf1N1B4_1626 0.27 -0.9 1,869,777 - Pf1N1B4_1626 0.27 +0.3 1,869,777 - Pf1N1B4_1626 0.27 -0.8 1,869,777 - Pf1N1B4_1626 0.27 -3.3 1,869,777 - Pf1N1B4_1626 0.27 +0.3 1,869,777 - Pf1N1B4_1626 0.27 -0.7 1,869,777 - Pf1N1B4_1626 0.27 -3.0 1,869,777 - Pf1N1B4_1626 0.27 -1.8 1,869,777 - Pf1N1B4_1626 0.27 -0.3 1,869,777 - Pf1N1B4_1626 0.27 -0.7 1,869,777 - Pf1N1B4_1626 0.27 -1.8 1,869,777 - Pf1N1B4_1626 0.27 -1.5 1,869,777 - Pf1N1B4_1626 0.27 -2.3 1,869,777 - Pf1N1B4_1626 0.27 -2.0 1,869,779 + Pf1N1B4_1626 0.27 -0.7 1,869,779 + Pf1N1B4_1626 0.27 -1.3 1,869,779 + +0.3 1,869,779 + Pf1N1B4_1626 0.27 -1.7 1,869,779 + Pf1N1B4_1626 0.27 -0.7 1,869,779 + Pf1N1B4_1626 0.27 -2.5 1,869,779 + Pf1N1B4_1626 0.27 -2.3 1,869,780 - Pf1N1B4_1626 0.28 -1.7 1,869,780 - Pf1N1B4_1626 0.28 -0.7 1,869,780 - Pf1N1B4_1626 0.28 -2.1 1,869,780 - Pf1N1B4_1626 0.28 -1.5 1,869,780 - Pf1N1B4_1626 0.28 -1.0 1,869,795 - Pf1N1B4_1626 0.30 -1.8 1,869,795 - Pf1N1B4_1626 0.30 -3.8 1,869,795 - Pf1N1B4_1626 0.30 -1.0 1,869,795 - Pf1N1B4_1626 0.30 -0.9 1,869,795 - Pf1N1B4_1626 0.30 -1.7 1,869,839 - Pf1N1B4_1626 0.37 -0.7 1,869,839 - Pf1N1B4_1626 0.37 -2.8 1,869,839 - Pf1N1B4_1626 0.37 -2.7 1,870,466 + Pf1N1B4_1627 0.35 -1.7 1,870,468 + Pf1N1B4_1627 0.36 -1.6 1,870,468 + Pf1N1B4_1627 0.36 -2.0 1,870,469 - Pf1N1B4_1627 0.36 -0.7 1,870,469 - Pf1N1B4_1627 0.36 -1.7 1,870,546 + Pf1N1B4_1627 0.48 -2.0 1,870,546 + Pf1N1B4_1627 0.48 -1.5 1,870,546 + Pf1N1B4_1627 0.48 -0.3 1,870,547 - Pf1N1B4_1627 0.48 -1.3 1,870,547 - Pf1N1B4_1627 0.48 -1.7 1,870,547 - Pf1N1B4_1627 0.48 -1.7 1,870,547 - Pf1N1B4_1627 0.48 -0.7
Or see this region's nucleotide sequence