Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1604 and Pf1N1B4_1605 are separated by 174 nucleotides Pf1N1B4_1605 and Pf1N1B4_1606 overlap by 33 nucleotides Pf1N1B4_1606 and Pf1N1B4_1607 are separated by 353 nucleotides Pf1N1B4_1607 and Pf1N1B4_1608 are separated by 73 nucleotides
Pf1N1B4_1604 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 1,849,392 to 1,850,846
_1604
Pf1N1B4_1605 - hypothetical protein, at 1,851,021 to 1,851,209
_1605
Pf1N1B4_1606 - Glutamine synthetase type I (EC 6.3.1.2), at 1,851,177 to 1,852,583
_1606
Pf1N1B4_1607 - hypothetical protein, at 1,852,937 to 1,853,185
_1607
Pf1N1B4_1608 - FIG00956533: hypothetical protein, at 1,853,259 to 1,853,726
_1608
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1850.892 kb on - strand at 1851.022 kb on + strand at 1851.022 kb on + strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.023 kb on - strand at 1851.024 kb on + strand at 1851.024 kb on + strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1851.025 kb on - strand at 1852.645 kb on - strand at 1852.645 kb on - strand at 1852.687 kb on + strand at 1852.687 kb on + strand at 1852.688 kb on - strand at 1852.688 kb on - strand at 1852.739 kb on + strand at 1852.740 kb on - strand at 1852.740 kb on - strand at 1852.788 kb on + strand at 1852.789 kb on - strand at 1852.789 kb on - strand at 1852.891 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.901 kb on + strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1852.902 kb on - strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.006 kb on + strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.007 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.009 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.026 kb on - strand at 1853.026 kb on - strand, within Pf1N1B4_1607 at 1853.062 kb on + strand, within Pf1N1B4_1607 at 1853.063 kb on - strand, within Pf1N1B4_1607 at 1853.098 kb on + strand, within Pf1N1B4_1607 at 1853.099 kb on - strand, within Pf1N1B4_1607 at 1853.140 kb on + strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.141 kb on - strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.150 kb on + strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.151 kb on - strand, within Pf1N1B4_1607 at 1853.167 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.183 kb on + strand at 1853.184 kb on - strand at 1853.184 kb on - strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.224 kb on + strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.225 kb on - strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.227 kb on + strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.228 kb on - strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.233 kb on + strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.234 kb on - strand at 1853.271 kb on + strand at 1853.271 kb on + strand at 1853.272 kb on - strand at 1853.272 kb on - strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.277 kb on + strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.278 kb on - strand at 1853.515 kb on + strand, within Pf1N1B4_1608 at 1853.560 kb on + strand, within Pf1N1B4_1608 at 1853.561 kb on - strand, within Pf1N1B4_1608
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,850,892 - -0.5 1,851,022 + -0.9 1,851,022 + +1.0 1,851,023 - -0.9 1,851,023 - -2.5 1,851,023 - +1.6 1,851,023 - -1.3 1,851,023 - -1.7 1,851,023 - -0.7 1,851,023 - -1.0 1,851,023 - -2.0 1,851,023 - +0.3 1,851,023 - -0.7 1,851,024 + +2.6 1,851,024 + +1.3 1,851,025 - -0.7 1,851,025 - +1.3 1,851,025 - +0.3 1,851,025 - -1.3 1,851,025 - +0.0 1,851,025 - +0.9 1,851,025 - -1.1 1,851,025 - +0.9 1,851,025 - -0.7 1,851,025 - -2.2 1,851,025 - -0.6 1,852,645 - -0.4 1,852,645 - +0.4 1,852,687 + +0.3 1,852,687 + +0.3 1,852,688 - +0.3 1,852,688 - -1.0 1,852,739 + -0.1 1,852,740 - -0.4 1,852,740 - -0.7 1,852,788 + -1.7 1,852,789 - +2.9 1,852,789 - -0.1 1,852,891 + -1.3 1,852,901 + -0.7 1,852,901 + +1.3 1,852,901 + -1.4 1,852,902 - +1.8 1,852,902 - +0.3 1,852,902 - -0.0 1,852,902 - +0.5 1,852,902 - -0.3 1,852,902 - -1.3 1,852,902 - +0.9 1,852,902 - -2.3 1,852,902 - +0.3 1,853,006 + Pf1N1B4_1607 0.28 -0.4 1,853,006 + +1.1 1,853,006 + Pf1N1B4_1607 0.28 -0.3 1,853,006 + Pf1N1B4_1607 0.28 -3.8 1,853,006 + Pf1N1B4_1607 0.28 +2.0 1,853,006 + Pf1N1B4_1607 0.28 -0.0 1,853,006 + Pf1N1B4_1607 0.28 +0.7 1,853,006 + Pf1N1B4_1607 0.28 -1.3 1,853,006 + Pf1N1B4_1607 0.28 -1.5 1,853,006 + Pf1N1B4_1607 0.28 +0.6 1,853,006 + Pf1N1B4_1607 0.28 -2.0 1,853,006 + Pf1N1B4_1607 0.28 +2.3 1,853,006 + Pf1N1B4_1607 0.28 +3.1 1,853,006 + Pf1N1B4_1607 0.28 +1.4 1,853,007 - Pf1N1B4_1607 0.28 +0.8 1,853,007 - Pf1N1B4_1607 0.28 -0.4 1,853,007 - Pf1N1B4_1607 0.28 -1.4 1,853,007 - Pf1N1B4_1607 0.28 +0.1 1,853,007 - Pf1N1B4_1607 0.28 +1.9 1,853,007 - Pf1N1B4_1607 0.28 +0.3 1,853,007 - Pf1N1B4_1607 0.28 +0.8 1,853,007 - Pf1N1B4_1607 0.28 +0.3 1,853,007 - Pf1N1B4_1607 0.28 -0.3 1,853,007 - Pf1N1B4_1607 0.28 +0.7 1,853,007 - Pf1N1B4_1607 0.28 -1.7 1,853,007 - Pf1N1B4_1607 0.28 -1.3 1,853,007 - Pf1N1B4_1607 0.28 -1.5 1,853,007 - Pf1N1B4_1607 0.28 -1.3 1,853,007 - Pf1N1B4_1607 0.28 +1.3 1,853,007 - Pf1N1B4_1607 0.28 +0.0 1,853,007 - Pf1N1B4_1607 0.28 +1.9 1,853,007 - Pf1N1B4_1607 0.28 -1.7 1,853,007 - Pf1N1B4_1607 0.28 +1.0 1,853,007 - Pf1N1B4_1607 0.28 +0.0 1,853,007 - Pf1N1B4_1607 0.28 -2.5 1,853,007 - Pf1N1B4_1607 0.28 -2.0 1,853,007 - Pf1N1B4_1607 0.28 -0.7 1,853,007 - Pf1N1B4_1607 0.28 +0.7 1,853,009 - Pf1N1B4_1607 0.29 -2.6 1,853,009 - Pf1N1B4_1607 0.29 -1.0 1,853,009 - Pf1N1B4_1607 0.29 -1.3 1,853,009 - Pf1N1B4_1607 0.29 +0.3 1,853,009 - Pf1N1B4_1607 0.29 +1.2 1,853,026 - Pf1N1B4_1607 0.36 +0.8 1,853,026 - Pf1N1B4_1607 0.36 -1.3 1,853,026 - +1.3 1,853,026 - Pf1N1B4_1607 0.36 -0.3 1,853,062 + Pf1N1B4_1607 0.50 -0.3 1,853,063 - Pf1N1B4_1607 0.51 +0.1 1,853,098 + Pf1N1B4_1607 0.65 +1.5 1,853,099 - Pf1N1B4_1607 0.65 +0.0 1,853,140 + Pf1N1B4_1607 0.82 +0.6 1,853,141 - Pf1N1B4_1607 0.82 +0.6 1,853,141 - Pf1N1B4_1607 0.82 -1.5 1,853,141 - Pf1N1B4_1607 0.82 +1.8 1,853,141 - Pf1N1B4_1607 0.82 -1.7 1,853,141 - Pf1N1B4_1607 0.82 -0.7 1,853,150 + Pf1N1B4_1607 0.86 -0.3 1,853,150 + Pf1N1B4_1607 0.86 -0.1 1,853,150 + Pf1N1B4_1607 0.86 +3.5 1,853,150 + Pf1N1B4_1607 0.86 -0.1 1,853,151 - Pf1N1B4_1607 0.86 +1.6 1,853,151 - Pf1N1B4_1607 0.86 -1.4 1,853,151 - Pf1N1B4_1607 0.86 +1.0 1,853,151 - Pf1N1B4_1607 0.86 -2.0 1,853,151 - Pf1N1B4_1607 0.86 +1.3 1,853,151 - Pf1N1B4_1607 0.86 -2.0 1,853,151 - Pf1N1B4_1607 0.86 +0.9 1,853,151 - Pf1N1B4_1607 0.86 -0.9 1,853,151 - Pf1N1B4_1607 0.86 -2.5 1,853,167 + -2.3 1,853,183 + +0.7 1,853,183 + -0.3 1,853,183 + +0.1 1,853,184 - -0.7 1,853,184 - +0.5 1,853,224 + +0.3 1,853,224 + +0.3 1,853,224 + -1.3 1,853,225 - -0.7 1,853,225 - -0.5 1,853,225 - +0.1 1,853,227 + -0.6 1,853,227 + -1.7 1,853,227 + +1.9 1,853,227 + +0.3 1,853,227 + +0.3 1,853,227 + +2.6 1,853,228 - +1.3 1,853,228 - -0.3 1,853,228 - +1.3 1,853,228 - -0.1 1,853,228 - -0.2 1,853,228 - -2.0 1,853,228 - -1.7 1,853,228 - -1.0 1,853,228 - -0.7 1,853,228 - +0.3 1,853,228 - +1.3 1,853,228 - +1.6 1,853,228 - -0.0 1,853,233 + +0.2 1,853,233 + +1.9 1,853,233 + +1.9 1,853,233 + -0.5 1,853,234 - +0.3 1,853,234 - -1.0 1,853,234 - -0.3 1,853,271 + +1.3 1,853,271 + +0.9 1,853,272 - -0.7 1,853,272 - +0.3 1,853,277 + -1.7 1,853,277 + +2.6 1,853,277 + +0.9 1,853,277 + +0.3 1,853,277 + -0.1 1,853,277 + +0.5 1,853,277 + +0.0 1,853,277 + -0.4 1,853,277 + -0.7 1,853,277 + -1.8 1,853,278 - +0.4 1,853,278 - +2.1 1,853,278 - -1.7 1,853,278 - +0.4 1,853,278 - +0.3 1,853,278 - +0.1 1,853,278 - -2.1 1,853,278 - +1.9 1,853,278 - +0.1 1,853,515 + Pf1N1B4_1608 0.55 +0.3 1,853,560 + Pf1N1B4_1608 0.64 +0.4 1,853,561 - Pf1N1B4_1608 0.65 -0.7
Or see this region's nucleotide sequence