Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1564 and Pf1N1B4_1565 are separated by 90 nucleotides Pf1N1B4_1565 and Pf1N1B4_1566 overlap by 8 nucleotides Pf1N1B4_1566 and Pf1N1B4_1567 are separated by 24 nucleotides Pf1N1B4_1567 and Pf1N1B4_1568 are separated by 10 nucleotides
Pf1N1B4_1564 - Ubiquinone biosynthesis monooxygenase UbiB, at 1,801,976 to 1,803,580
_1564
Pf1N1B4_1565 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 1,803,671 to 1,804,072
_1565
Pf1N1B4_1566 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 1,804,065 to 1,804,397
_1566
Pf1N1B4_1567 - Twin-arginine translocation protein TatA, at 1,804,422 to 1,804,700
_1567
Pf1N1B4_1568 - Twin-arginine translocation protein TatB, at 1,804,711 to 1,805,136
_1568
Position (kb)
1803
1804
1805 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.640 kb on + strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.641 kb on - strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.648 kb on + strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.649 kb on - strand at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.818 kb on + strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.819 kb on - strand, within Pf1N1B4_1565 at 1803.980 kb on + strand, within Pf1N1B4_1565 at 1803.981 kb on - strand, within Pf1N1B4_1565 at 1803.981 kb on - strand, within Pf1N1B4_1565 at 1804.028 kb on - strand at 1804.028 kb on - strand, within Pf1N1B4_1565 at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.043 kb on - strand at 1804.046 kb on + strand at 1804.046 kb on + strand at 1804.046 kb on + strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.047 kb on - strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.060 kb on + strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.061 kb on - strand at 1804.133 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.134 kb on + strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.135 kb on - strand, within Pf1N1B4_1566 at 1804.149 kb on + strand, within Pf1N1B4_1566 at 1804.150 kb on - strand, within Pf1N1B4_1566 at 1804.150 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.258 kb on - strand, within Pf1N1B4_1566 at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.403 kb on + strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.404 kb on - strand at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.541 kb on + strand, within Pf1N1B4_1567 at 1804.542 kb on - strand, within Pf1N1B4_1567 at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.709 kb on + strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.710 kb on - strand at 1804.719 kb on + strand at 1804.719 kb on + strand at 1804.719 kb on + strand at 1804.720 kb on - strand at 1804.720 kb on - strand at 1804.986 kb on - strand, within Pf1N1B4_1568 at 1805.060 kb on + strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,803,640 + +2.9 1,803,640 + +2.1 1,803,640 + -0.0 1,803,640 + -2.5 1,803,641 - -0.2 1,803,641 - +0.3 1,803,641 - -1.9 1,803,641 - -0.3 1,803,641 - -2.5 1,803,641 - -1.3 1,803,641 - -2.0 1,803,641 - -1.3 1,803,641 - -2.3 1,803,641 - +0.6 1,803,641 - -1.1 1,803,641 - -1.5 1,803,641 - +0.6 1,803,648 + +0.6 1,803,648 + +1.9 1,803,648 + +0.1 1,803,648 + +1.3 1,803,648 + +1.6 1,803,649 - +0.3 1,803,649 - -1.5 1,803,649 - -1.5 1,803,649 - -1.3 1,803,649 - +0.9 1,803,649 - -1.7 1,803,649 - -0.9 1,803,649 - -0.4 1,803,818 + Pf1N1B4_1565 0.37 +0.3 1,803,818 + Pf1N1B4_1565 0.37 -4.0 1,803,818 + Pf1N1B4_1565 0.37 +0.7 1,803,818 + Pf1N1B4_1565 0.37 -2.3 1,803,819 - Pf1N1B4_1565 0.37 -1.3 1,803,819 - Pf1N1B4_1565 0.37 -2.5 1,803,819 - Pf1N1B4_1565 0.37 -2.5 1,803,819 - Pf1N1B4_1565 0.37 +0.3 1,803,819 - Pf1N1B4_1565 0.37 -1.7 1,803,819 - Pf1N1B4_1565 0.37 -1.3 1,803,980 + Pf1N1B4_1565 0.77 -1.7 1,803,981 - Pf1N1B4_1565 0.77 +0.3 1,803,981 - Pf1N1B4_1565 0.77 -1.7 1,804,028 - +1.3 1,804,028 - Pf1N1B4_1565 0.89 -0.0 1,804,043 - -0.3 1,804,043 - +0.1 1,804,043 - +0.7 1,804,043 - -0.0 1,804,043 - -0.3 1,804,046 + -1.3 1,804,046 + +0.3 1,804,046 + +1.3 1,804,047 - -1.7 1,804,047 - +0.6 1,804,047 - +0.3 1,804,047 - -1.7 1,804,047 - +0.3 1,804,047 - -1.4 1,804,060 + -0.7 1,804,060 + -1.5 1,804,060 + +1.9 1,804,060 + -1.3 1,804,061 - -0.2 1,804,061 - -0.1 1,804,061 - -1.0 1,804,061 - -1.4 1,804,061 - +0.3 1,804,061 - +0.1 1,804,061 - +0.9 1,804,061 - -1.4 1,804,061 - +0.7 1,804,061 - -0.4 1,804,061 - -1.3 1,804,061 - +0.6 1,804,061 - -0.3 1,804,061 - -0.1 1,804,061 - -1.4 1,804,061 - -0.3 1,804,061 - -1.3 1,804,061 - -0.7 1,804,133 + Pf1N1B4_1566 0.20 -0.7 1,804,134 + Pf1N1B4_1566 0.21 +1.0 1,804,134 + Pf1N1B4_1566 0.21 +0.1 1,804,134 + +2.6 1,804,134 + Pf1N1B4_1566 0.21 +0.9 1,804,134 + Pf1N1B4_1566 0.21 +0.7 1,804,134 + Pf1N1B4_1566 0.21 -0.4 1,804,134 + Pf1N1B4_1566 0.21 -0.7 1,804,135 - Pf1N1B4_1566 0.21 -0.1 1,804,135 - Pf1N1B4_1566 0.21 +0.5 1,804,135 - Pf1N1B4_1566 0.21 -0.8 1,804,135 - Pf1N1B4_1566 0.21 +0.3 1,804,135 - Pf1N1B4_1566 0.21 -2.3 1,804,135 - Pf1N1B4_1566 0.21 +1.0 1,804,135 - Pf1N1B4_1566 0.21 +0.9 1,804,135 - Pf1N1B4_1566 0.21 -0.0 1,804,135 - Pf1N1B4_1566 0.21 +1.3 1,804,135 - Pf1N1B4_1566 0.21 +0.3 1,804,135 - Pf1N1B4_1566 0.21 +0.5 1,804,135 - Pf1N1B4_1566 0.21 +1.8 1,804,135 - Pf1N1B4_1566 0.21 -0.3 1,804,135 - Pf1N1B4_1566 0.21 +0.1 1,804,135 - Pf1N1B4_1566 0.21 -0.8 1,804,135 - Pf1N1B4_1566 0.21 +0.3 1,804,135 - Pf1N1B4_1566 0.21 +0.9 1,804,135 - Pf1N1B4_1566 0.21 +0.6 1,804,135 - Pf1N1B4_1566 0.21 +1.3 1,804,135 - Pf1N1B4_1566 0.21 -0.4 1,804,135 - Pf1N1B4_1566 0.21 +0.3 1,804,135 - Pf1N1B4_1566 0.21 +0.1 1,804,135 - Pf1N1B4_1566 0.21 -0.7 1,804,135 - Pf1N1B4_1566 0.21 -0.8 1,804,149 + Pf1N1B4_1566 0.25 -1.4 1,804,150 - Pf1N1B4_1566 0.26 -0.3 1,804,150 - Pf1N1B4_1566 0.26 +0.3 1,804,258 - Pf1N1B4_1566 0.58 +0.4 1,804,258 - Pf1N1B4_1566 0.58 -1.4 1,804,258 - Pf1N1B4_1566 0.58 -0.4 1,804,403 + +1.3 1,804,403 + -0.7 1,804,403 + +0.7 1,804,403 + +0.8 1,804,404 - -0.3 1,804,404 - +0.3 1,804,404 - -1.5 1,804,404 - +0.0 1,804,404 - -1.1 1,804,404 - -1.3 1,804,404 - +0.9 1,804,404 - +0.3 1,804,404 - -0.3 1,804,404 - +2.2 1,804,404 - +0.3 1,804,404 - -0.6 1,804,404 - +2.3 1,804,404 - -2.5 1,804,404 - +2.6 1,804,404 - +0.9 1,804,404 - -0.3 1,804,404 - +0.3 1,804,404 - +0.3 1,804,541 + Pf1N1B4_1567 0.43 +1.6 1,804,541 + Pf1N1B4_1567 0.43 +0.3 1,804,541 + Pf1N1B4_1567 0.43 -0.7 1,804,542 - Pf1N1B4_1567 0.43 -1.3 1,804,709 + -0.9 1,804,709 + -0.4 1,804,709 + -0.5 1,804,709 + -0.4 1,804,709 + -0.5 1,804,709 + -2.3 1,804,709 + -0.1 1,804,709 + +0.8 1,804,709 + -0.7 1,804,709 + +1.1 1,804,710 - -1.5 1,804,710 - -1.5 1,804,710 - +1.9 1,804,710 - +2.0 1,804,710 - -0.4 1,804,710 - -0.3 1,804,710 - +0.6 1,804,710 - -0.7 1,804,710 - -0.2 1,804,710 - +1.0 1,804,719 + +0.8 1,804,719 + -2.1 1,804,719 + -0.9 1,804,720 - -0.7 1,804,720 - -0.7 1,804,986 - Pf1N1B4_1568 0.65 -1.2 1,805,060 + Pf1N1B4_1568 0.82 +1.5 1,805,061 - Pf1N1B4_1568 0.82 +0.7 1,805,061 - Pf1N1B4_1568 0.82 +0.0 1,805,061 - Pf1N1B4_1568 0.82 +0.5 1,805,061 - Pf1N1B4_1568 0.82 -0.8 1,805,061 - Pf1N1B4_1568 0.82 +1.3 1,805,061 - Pf1N1B4_1568 0.82 +0.3 1,805,061 - Pf1N1B4_1568 0.82 +0.9
Or see this region's nucleotide sequence