Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1555 and Pf1N1B4_1556 are separated by 134 nucleotides Pf1N1B4_1556 and Pf1N1B4_1557 are separated by 129 nucleotides
Pf1N1B4_1555 - Polyhydroxyalkanoic acid synthase, at 1,793,254 to 1,794,933
_1555
Pf1N1B4_1556 - Poly(3-hydroxyalkanoate) depolymerase, at 1,795,068 to 1,795,922
_1556
Pf1N1B4_1557 - Polyhydroxyalkanoic acid synthase, at 1,796,052 to 1,797,734
_1557
Position (kb)
1795
1796 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1794.105 kb on + strand, within Pf1N1B4_1555 at 1794.385 kb on + strand, within Pf1N1B4_1555 at 1794.386 kb on - strand, within Pf1N1B4_1555 at 1794.386 kb on - strand, within Pf1N1B4_1555 at 1794.400 kb on + strand, within Pf1N1B4_1555 at 1794.400 kb on + strand, within Pf1N1B4_1555 at 1794.400 kb on + strand, within Pf1N1B4_1555 at 1794.400 kb on + strand, within Pf1N1B4_1555 at 1794.400 kb on + strand, within Pf1N1B4_1555 at 1794.401 kb on - strand, within Pf1N1B4_1555 at 1794.401 kb on - strand, within Pf1N1B4_1555 at 1794.401 kb on - strand, within Pf1N1B4_1555 at 1794.401 kb on - strand, within Pf1N1B4_1555 at 1794.401 kb on - strand at 1794.401 kb on - strand, within Pf1N1B4_1555 at 1794.632 kb on - strand, within Pf1N1B4_1555 at 1794.690 kb on + strand, within Pf1N1B4_1555 at 1794.691 kb on - strand, within Pf1N1B4_1555 at 1794.733 kb on + strand, within Pf1N1B4_1555 at 1794.733 kb on + strand, within Pf1N1B4_1555 at 1794.733 kb on + strand, within Pf1N1B4_1555 at 1794.734 kb on - strand, within Pf1N1B4_1555 at 1794.734 kb on - strand, within Pf1N1B4_1555 at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.916 kb on + strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.917 kb on - strand at 1794.920 kb on + strand at 1794.920 kb on + strand at 1794.920 kb on + strand at 1794.921 kb on - strand at 1794.942 kb on + strand at 1794.981 kb on + strand at 1794.981 kb on + strand at 1795.198 kb on + strand, within Pf1N1B4_1556 at 1795.199 kb on - strand at 1795.199 kb on - strand, within Pf1N1B4_1556 at 1795.335 kb on + strand, within Pf1N1B4_1556 at 1795.335 kb on + strand, within Pf1N1B4_1556 at 1795.515 kb on + strand, within Pf1N1B4_1556 at 1795.515 kb on + strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.516 kb on - strand, within Pf1N1B4_1556 at 1795.557 kb on + strand, within Pf1N1B4_1556 at 1795.558 kb on - strand, within Pf1N1B4_1556 at 1795.638 kb on + strand, within Pf1N1B4_1556 at 1795.638 kb on + strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.639 kb on - strand, within Pf1N1B4_1556 at 1795.888 kb on + strand at 1795.889 kb on - strand at 1795.889 kb on - strand at 1795.942 kb on - strand at 1795.942 kb on - strand at 1795.994 kb on + strand at 1795.995 kb on - strand at 1795.995 kb on - strand at 1796.046 kb on + strand at 1796.046 kb on + strand at 1796.047 kb on - strand at 1796.193 kb on + strand at 1796.193 kb on + strand at 1796.194 kb on - strand at 1796.194 kb on - strand at 1796.194 kb on - strand at 1796.240 kb on - strand, within Pf1N1B4_1557 at 1796.320 kb on - strand, within Pf1N1B4_1557 at 1796.340 kb on + strand, within Pf1N1B4_1557 at 1796.340 kb on + strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.341 kb on - strand, within Pf1N1B4_1557 at 1796.511 kb on - strand, within Pf1N1B4_1557 at 1796.511 kb on - strand, within Pf1N1B4_1557 at 1796.667 kb on + strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.668 kb on - strand, within Pf1N1B4_1557 at 1796.691 kb on + strand, within Pf1N1B4_1557 at 1796.692 kb on - strand, within Pf1N1B4_1557 at 1796.705 kb on - strand, within Pf1N1B4_1557 at 1796.733 kb on + strand, within Pf1N1B4_1557 at 1796.733 kb on + strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.734 kb on - strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.736 kb on + strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.737 kb on - strand, within Pf1N1B4_1557 at 1796.859 kb on + strand, within Pf1N1B4_1557 at 1796.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,794,105 + Pf1N1B4_1555 0.51 +0.4 1,794,385 + Pf1N1B4_1555 0.67 -1.7 1,794,386 - Pf1N1B4_1555 0.67 -1.7 1,794,386 - Pf1N1B4_1555 0.67 +0.3 1,794,400 + Pf1N1B4_1555 0.68 -1.3 1,794,400 + Pf1N1B4_1555 0.68 -0.0 1,794,400 + Pf1N1B4_1555 0.68 +0.3 1,794,400 + Pf1N1B4_1555 0.68 +1.2 1,794,400 + Pf1N1B4_1555 0.68 -2.0 1,794,401 - Pf1N1B4_1555 0.68 +1.8 1,794,401 - Pf1N1B4_1555 0.68 +0.1 1,794,401 - Pf1N1B4_1555 0.68 -1.0 1,794,401 - Pf1N1B4_1555 0.68 -0.4 1,794,401 - +2.6 1,794,401 - Pf1N1B4_1555 0.68 +1.3 1,794,632 - Pf1N1B4_1555 0.82 -0.7 1,794,690 + Pf1N1B4_1555 0.85 +0.1 1,794,691 - Pf1N1B4_1555 0.86 +0.9 1,794,733 + Pf1N1B4_1555 0.88 -0.1 1,794,733 + Pf1N1B4_1555 0.88 +0.0 1,794,733 + Pf1N1B4_1555 0.88 +1.2 1,794,734 - Pf1N1B4_1555 0.88 +0.4 1,794,734 - Pf1N1B4_1555 0.88 -2.2 1,794,916 + +1.9 1,794,916 + -1.7 1,794,916 + +0.3 1,794,916 + +0.7 1,794,916 + -1.2 1,794,916 + +0.0 1,794,916 + -1.0 1,794,916 + -1.0 1,794,916 + +0.3 1,794,916 + -2.0 1,794,916 + -0.7 1,794,916 + -0.7 1,794,916 + -0.4 1,794,916 + -0.7 1,794,916 + +1.9 1,794,916 + -1.0 1,794,917 - +0.1 1,794,917 - +0.7 1,794,917 - +0.8 1,794,917 - +0.3 1,794,917 - -0.9 1,794,917 - +1.6 1,794,917 - -1.3 1,794,917 - +1.3 1,794,917 - +0.7 1,794,917 - -2.9 1,794,917 - -1.3 1,794,920 + +0.3 1,794,920 + -1.2 1,794,920 + +0.1 1,794,921 - +1.1 1,794,942 + -1.0 1,794,981 + -2.0 1,794,981 + -2.0 1,795,198 + Pf1N1B4_1556 0.15 -0.3 1,795,199 - +0.9 1,795,199 - Pf1N1B4_1556 0.15 -1.5 1,795,335 + Pf1N1B4_1556 0.31 -1.0 1,795,335 + Pf1N1B4_1556 0.31 -2.1 1,795,515 + Pf1N1B4_1556 0.52 +1.3 1,795,515 + Pf1N1B4_1556 0.52 +0.3 1,795,516 - Pf1N1B4_1556 0.52 +1.0 1,795,516 - Pf1N1B4_1556 0.52 -1.7 1,795,516 - Pf1N1B4_1556 0.52 +0.0 1,795,557 + Pf1N1B4_1556 0.57 -0.2 1,795,558 - Pf1N1B4_1556 0.57 -1.0 1,795,638 + Pf1N1B4_1556 0.67 +1.0 1,795,638 + Pf1N1B4_1556 0.67 +0.7 1,795,639 - Pf1N1B4_1556 0.67 -0.5 1,795,639 - Pf1N1B4_1556 0.67 -0.9 1,795,639 - Pf1N1B4_1556 0.67 -0.3 1,795,888 + +0.3 1,795,889 - +1.3 1,795,889 - -0.7 1,795,942 - +0.3 1,795,942 - +0.3 1,795,994 + +1.3 1,795,995 - -0.4 1,795,995 - -1.7 1,796,046 + -1.3 1,796,046 + -1.3 1,796,047 - +1.5 1,796,193 + -0.2 1,796,193 + -0.7 1,796,194 - -1.3 1,796,194 - -0.7 1,796,194 - -0.5 1,796,240 - Pf1N1B4_1557 0.11 +0.2 1,796,320 - Pf1N1B4_1557 0.16 -1.3 1,796,340 + Pf1N1B4_1557 0.17 -1.4 1,796,340 + Pf1N1B4_1557 0.17 +1.3 1,796,341 - Pf1N1B4_1557 0.17 +0.3 1,796,341 - Pf1N1B4_1557 0.17 +2.1 1,796,341 - Pf1N1B4_1557 0.17 +1.3 1,796,341 - Pf1N1B4_1557 0.17 -2.5 1,796,511 - Pf1N1B4_1557 0.27 +0.1 1,796,511 - Pf1N1B4_1557 0.27 +0.6 1,796,667 + Pf1N1B4_1557 0.37 -0.9 1,796,668 - Pf1N1B4_1557 0.37 -0.1 1,796,668 - Pf1N1B4_1557 0.37 +0.9 1,796,668 - Pf1N1B4_1557 0.37 +0.6 1,796,668 - Pf1N1B4_1557 0.37 +1.0 1,796,691 + Pf1N1B4_1557 0.38 -0.0 1,796,692 - Pf1N1B4_1557 0.38 +1.1 1,796,705 - Pf1N1B4_1557 0.39 +0.3 1,796,733 + Pf1N1B4_1557 0.40 +0.2 1,796,733 + Pf1N1B4_1557 0.40 -0.5 1,796,734 - Pf1N1B4_1557 0.41 +2.4 1,796,734 - Pf1N1B4_1557 0.41 -1.7 1,796,734 - Pf1N1B4_1557 0.41 +0.3 1,796,734 - Pf1N1B4_1557 0.41 +1.3 1,796,734 - Pf1N1B4_1557 0.41 +0.7 1,796,736 + Pf1N1B4_1557 0.41 +3.5 1,796,736 + Pf1N1B4_1557 0.41 +2.0 1,796,736 + Pf1N1B4_1557 0.41 -0.7 1,796,736 + Pf1N1B4_1557 0.41 +2.3 1,796,736 + Pf1N1B4_1557 0.41 -0.1 1,796,736 + Pf1N1B4_1557 0.41 -0.5 1,796,737 - Pf1N1B4_1557 0.41 +0.4 1,796,737 - Pf1N1B4_1557 0.41 -0.7 1,796,737 - Pf1N1B4_1557 0.41 -0.7 1,796,737 - Pf1N1B4_1557 0.41 +2.3 1,796,859 + Pf1N1B4_1557 0.48 +1.3 1,796,860 - +0.3
Or see this region's nucleotide sequence