Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1306 and Pf1N1B4_1307 are separated by 132 nucleotides Pf1N1B4_1307 and Pf1N1B4_1308 are separated by 49 nucleotides Pf1N1B4_1308 and Pf1N1B4_1309 are separated by 17 nucleotides
Pf1N1B4_1306 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 1,489,168 to 1,490,907
_1306
Pf1N1B4_1307 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 1,491,040 to 1,491,492
_1307
Pf1N1B4_1308 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 1,491,542 to 1,492,009
_1308
Pf1N1B4_1309 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 1,492,027 to 1,493,385
_1309
Position (kb)
1491
1492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1490.072 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.101 kb on + strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.102 kb on - strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.236 kb on + strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.237 kb on - strand, within Pf1N1B4_1306 at 1490.359 kb on - strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.380 kb on + strand, within Pf1N1B4_1306 at 1490.381 kb on - strand, within Pf1N1B4_1306 at 1490.381 kb on - strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.382 kb on + strand, within Pf1N1B4_1306 at 1490.383 kb on - strand, within Pf1N1B4_1306 at 1490.383 kb on - strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.434 kb on + strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.435 kb on - strand, within Pf1N1B4_1306 at 1490.453 kb on - strand, within Pf1N1B4_1306 at 1490.557 kb on - strand, within Pf1N1B4_1306 at 1490.773 kb on + strand at 1490.773 kb on + strand at 1490.774 kb on - strand at 1490.774 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.781 kb on - strand at 1490.921 kb on + strand at 1490.921 kb on + strand at 1491.059 kb on + strand at 1491.182 kb on - strand, within Pf1N1B4_1307 at 1491.282 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307 at 1491.385 kb on + strand, within Pf1N1B4_1307
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,490,072 + Pf1N1B4_1306 0.52 +0.9 1,490,101 + Pf1N1B4_1306 0.54 -2.2 1,490,101 + Pf1N1B4_1306 0.54 +0.5 1,490,101 + Pf1N1B4_1306 0.54 +0.2 1,490,101 + Pf1N1B4_1306 0.54 -0.2 1,490,101 + Pf1N1B4_1306 0.54 -0.7 1,490,101 + Pf1N1B4_1306 0.54 -0.5 1,490,101 + Pf1N1B4_1306 0.54 -1.3 1,490,101 + Pf1N1B4_1306 0.54 -2.4 1,490,101 + Pf1N1B4_1306 0.54 -0.1 1,490,101 + Pf1N1B4_1306 0.54 -0.1 1,490,101 + Pf1N1B4_1306 0.54 +0.0 1,490,101 + Pf1N1B4_1306 0.54 -1.8 1,490,101 + Pf1N1B4_1306 0.54 +0.0 1,490,101 + Pf1N1B4_1306 0.54 +0.6 1,490,101 + Pf1N1B4_1306 0.54 -0.1 1,490,102 - Pf1N1B4_1306 0.54 -0.5 1,490,102 - Pf1N1B4_1306 0.54 +0.5 1,490,102 - Pf1N1B4_1306 0.54 +0.5 1,490,102 - Pf1N1B4_1306 0.54 -1.8 1,490,102 - Pf1N1B4_1306 0.54 -0.4 1,490,102 - Pf1N1B4_1306 0.54 +1.0 1,490,102 - Pf1N1B4_1306 0.54 +0.5 1,490,102 - Pf1N1B4_1306 0.54 -0.2 1,490,102 - Pf1N1B4_1306 0.54 +0.7 1,490,236 + Pf1N1B4_1306 0.61 +1.2 1,490,236 + Pf1N1B4_1306 0.61 +1.2 1,490,236 + Pf1N1B4_1306 0.61 -0.4 1,490,236 + Pf1N1B4_1306 0.61 +1.4 1,490,236 + Pf1N1B4_1306 0.61 -1.4 1,490,236 + Pf1N1B4_1306 0.61 -1.6 1,490,236 + Pf1N1B4_1306 0.61 -0.8 1,490,236 + Pf1N1B4_1306 0.61 -1.4 1,490,237 - Pf1N1B4_1306 0.61 +0.2 1,490,237 - Pf1N1B4_1306 0.61 -2.8 1,490,237 - Pf1N1B4_1306 0.61 +1.0 1,490,237 - Pf1N1B4_1306 0.61 -1.4 1,490,359 - Pf1N1B4_1306 0.68 +1.7 1,490,380 + Pf1N1B4_1306 0.70 -0.4 1,490,380 + Pf1N1B4_1306 0.70 +1.2 1,490,380 + Pf1N1B4_1306 0.70 -1.8 1,490,381 - Pf1N1B4_1306 0.70 +1.6 1,490,381 - Pf1N1B4_1306 0.70 -1.1 1,490,382 + Pf1N1B4_1306 0.70 -0.8 1,490,382 + Pf1N1B4_1306 0.70 +0.2 1,490,382 + Pf1N1B4_1306 0.70 -1.0 1,490,382 + Pf1N1B4_1306 0.70 -1.8 1,490,383 - Pf1N1B4_1306 0.70 +0.2 1,490,383 - Pf1N1B4_1306 0.70 +2.0 1,490,434 + Pf1N1B4_1306 0.73 -3.0 1,490,434 + Pf1N1B4_1306 0.73 -1.2 1,490,434 + Pf1N1B4_1306 0.73 -0.4 1,490,434 + Pf1N1B4_1306 0.73 -0.6 1,490,435 - Pf1N1B4_1306 0.73 +1.9 1,490,435 - Pf1N1B4_1306 0.73 +0.9 1,490,435 - Pf1N1B4_1306 0.73 -0.2 1,490,435 - Pf1N1B4_1306 0.73 -1.1 1,490,435 - Pf1N1B4_1306 0.73 -0.6 1,490,435 - Pf1N1B4_1306 0.73 -0.4 1,490,453 - Pf1N1B4_1306 0.74 -0.3 1,490,557 - Pf1N1B4_1306 0.80 +0.2 1,490,773 + -1.4 1,490,773 + -0.7 1,490,774 - +1.4 1,490,774 - +1.0 1,490,781 - +1.2 1,490,781 - -0.1 1,490,781 - -1.2 1,490,781 - -2.0 1,490,781 - -1.4 1,490,781 - -1.4 1,490,781 - -1.0 1,490,781 - -2.4 1,490,921 + +0.2 1,490,921 + -2.2 1,491,059 + -0.4 1,491,182 - Pf1N1B4_1307 0.31 -0.8 1,491,282 + Pf1N1B4_1307 0.53 -2.7 1,491,385 + Pf1N1B4_1307 0.76 -2.2 1,491,385 + Pf1N1B4_1307 0.76 +2.5 1,491,385 + Pf1N1B4_1307 0.76 -1.0
Or see this region's nucleotide sequence