Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1232 and Pf1N1B4_1233 overlap by 4 nucleotides Pf1N1B4_1233 and Pf1N1B4_1234 are separated by 118 nucleotides Pf1N1B4_1234 and Pf1N1B4_1235 overlap by 4 nucleotides
Pf1N1B4_1232 - Inner membrane protein YfiN, at 1,398,098 to 1,399,366
_1232
Pf1N1B4_1233 - Uncharacterized protein YfiR precursor, at 1,399,363 to 1,399,938
_1233
Pf1N1B4_1234 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 1,400,057 to 1,402,165
_1234
Pf1N1B4_1235 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 1,402,162 to 1,405,851
_1235
Position (kb)
1400
1401
1402
1403 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1399.070 kb on + strand, within Pf1N1B4_1232 at 1399.070 kb on + strand, within Pf1N1B4_1232 at 1399.071 kb on - strand, within Pf1N1B4_1232 at 1399.188 kb on + strand, within Pf1N1B4_1232 at 1399.188 kb on + strand, within Pf1N1B4_1232 at 1399.188 kb on + strand, within Pf1N1B4_1232 at 1399.189 kb on - strand, within Pf1N1B4_1232 at 1399.189 kb on - strand, within Pf1N1B4_1232 at 1399.189 kb on - strand, within Pf1N1B4_1232 at 1399.230 kb on + strand, within Pf1N1B4_1232 at 1399.231 kb on - strand, within Pf1N1B4_1232 at 1399.343 kb on + strand at 1399.343 kb on + strand at 1399.343 kb on + strand at 1399.343 kb on + strand at 1399.343 kb on + strand at 1399.344 kb on - strand at 1399.344 kb on - strand at 1399.344 kb on - strand at 1399.344 kb on - strand at 1399.344 kb on - strand at 1399.344 kb on - strand at 1399.359 kb on - strand at 1399.459 kb on + strand, within Pf1N1B4_1233 at 1399.459 kb on + strand, within Pf1N1B4_1233 at 1399.460 kb on - strand, within Pf1N1B4_1233 at 1399.460 kb on - strand, within Pf1N1B4_1233 at 1399.498 kb on + strand, within Pf1N1B4_1233 at 1399.499 kb on - strand, within Pf1N1B4_1233 at 1399.598 kb on + strand, within Pf1N1B4_1233 at 1399.598 kb on + strand, within Pf1N1B4_1233 at 1399.598 kb on + strand, within Pf1N1B4_1233 at 1399.599 kb on - strand, within Pf1N1B4_1233 at 1399.706 kb on + strand, within Pf1N1B4_1233 at 1399.706 kb on + strand, within Pf1N1B4_1233 at 1399.766 kb on + strand, within Pf1N1B4_1233 at 1399.766 kb on + strand, within Pf1N1B4_1233 at 1399.767 kb on - strand, within Pf1N1B4_1233 at 1400.013 kb on + strand at 1400.013 kb on + strand at 1400.199 kb on + strand at 1400.199 kb on + strand at 1400.199 kb on + strand at 1400.199 kb on + strand at 1400.200 kb on - strand at 1400.200 kb on - strand at 1400.200 kb on - strand at 1400.200 kb on - strand at 1400.286 kb on - strand, within Pf1N1B4_1234 at 1400.298 kb on + strand, within Pf1N1B4_1234 at 1400.298 kb on + strand, within Pf1N1B4_1234 at 1400.715 kb on + strand, within Pf1N1B4_1234 at 1400.807 kb on + strand, within Pf1N1B4_1234 at 1400.808 kb on - strand, within Pf1N1B4_1234 at 1400.835 kb on - strand, within Pf1N1B4_1234 at 1401.040 kb on - strand, within Pf1N1B4_1234 at 1401.209 kb on + strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.210 kb on - strand, within Pf1N1B4_1234 at 1401.658 kb on + strand, within Pf1N1B4_1234 at 1401.697 kb on - strand, within Pf1N1B4_1234 at 1401.697 kb on - strand, within Pf1N1B4_1234 at 1402.172 kb on - strand at 1402.172 kb on - strand at 1402.269 kb on - strand at 1402.293 kb on + strand at 1402.311 kb on - strand at 1402.311 kb on - strand at 1402.311 kb on - strand at 1402.389 kb on - strand at 1402.389 kb on - strand at 1402.397 kb on + strand at 1402.398 kb on - strand at 1402.398 kb on - strand at 1402.452 kb on - strand at 1402.499 kb on + strand at 1402.499 kb on + strand at 1402.500 kb on - strand at 1402.602 kb on - strand at 1402.602 kb on - strand, within Pf1N1B4_1235
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,399,070 + Pf1N1B4_1232 0.77 +1.6 1,399,070 + Pf1N1B4_1232 0.77 -2.6 1,399,071 - Pf1N1B4_1232 0.77 -0.4 1,399,188 + Pf1N1B4_1232 0.86 +0.7 1,399,188 + Pf1N1B4_1232 0.86 +1.0 1,399,188 + Pf1N1B4_1232 0.86 +2.2 1,399,189 - Pf1N1B4_1232 0.86 +0.2 1,399,189 - Pf1N1B4_1232 0.86 +1.6 1,399,189 - Pf1N1B4_1232 0.86 +0.2 1,399,230 + Pf1N1B4_1232 0.89 +1.1 1,399,231 - Pf1N1B4_1232 0.89 -0.4 1,399,343 + -1.4 1,399,343 + +0.7 1,399,343 + +0.2 1,399,343 + +0.2 1,399,343 + -1.4 1,399,344 - -1.0 1,399,344 - +0.0 1,399,344 - +1.1 1,399,344 - +2.2 1,399,344 - +0.2 1,399,344 - +0.6 1,399,359 - -2.4 1,399,459 + Pf1N1B4_1233 0.17 +0.2 1,399,459 + Pf1N1B4_1233 0.17 +0.2 1,399,460 - Pf1N1B4_1233 0.17 -0.8 1,399,460 - Pf1N1B4_1233 0.17 -0.4 1,399,498 + Pf1N1B4_1233 0.23 +0.7 1,399,499 - Pf1N1B4_1233 0.24 -0.8 1,399,598 + Pf1N1B4_1233 0.41 -0.4 1,399,598 + Pf1N1B4_1233 0.41 +1.0 1,399,598 + Pf1N1B4_1233 0.41 -0.4 1,399,599 - Pf1N1B4_1233 0.41 +0.2 1,399,706 + Pf1N1B4_1233 0.60 -0.2 1,399,706 + Pf1N1B4_1233 0.60 +0.5 1,399,766 + Pf1N1B4_1233 0.70 +0.2 1,399,766 + Pf1N1B4_1233 0.70 -0.1 1,399,767 - Pf1N1B4_1233 0.70 +1.5 1,400,013 + +0.2 1,400,013 + -0.7 1,400,199 + -1.4 1,400,199 + -0.4 1,400,199 + +1.2 1,400,199 + -0.8 1,400,200 - +0.2 1,400,200 - -1.8 1,400,200 - +1.7 1,400,200 - -2.6 1,400,286 - Pf1N1B4_1234 0.11 -0.8 1,400,298 + Pf1N1B4_1234 0.11 -2.0 1,400,298 + Pf1N1B4_1234 0.11 -2.4 1,400,715 + Pf1N1B4_1234 0.31 +0.2 1,400,807 + Pf1N1B4_1234 0.36 -1.2 1,400,808 - Pf1N1B4_1234 0.36 -2.2 1,400,835 - Pf1N1B4_1234 0.37 -1.8 1,401,040 - Pf1N1B4_1234 0.47 -2.7 1,401,209 + Pf1N1B4_1234 0.55 -0.8 1,401,210 - Pf1N1B4_1234 0.55 +1.2 1,401,210 - Pf1N1B4_1234 0.55 -0.1 1,401,210 - Pf1N1B4_1234 0.55 -1.4 1,401,210 - Pf1N1B4_1234 0.55 -1.2 1,401,658 + Pf1N1B4_1234 0.76 -1.4 1,401,697 - Pf1N1B4_1234 0.78 -3.0 1,401,697 - Pf1N1B4_1234 0.78 -1.8 1,402,172 - -0.4 1,402,172 - -0.4 1,402,269 - -3.2 1,402,293 + -2.7 1,402,311 - -1.8 1,402,311 - -0.2 1,402,311 - +1.2 1,402,389 - +1.2 1,402,389 - +0.2 1,402,397 + -2.4 1,402,398 - -1.2 1,402,398 - -0.3 1,402,452 - -0.4 1,402,499 + -0.3 1,402,499 + -2.0 1,402,500 - +1.2 1,402,602 - +0.2 1,402,602 - Pf1N1B4_1235 0.12 -1.7
Or see this region's nucleotide sequence