Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1180

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1178 and Pf1N1B4_1179 are separated by 75 nucleotidesPf1N1B4_1179 and Pf1N1B4_1180 are separated by 97 nucleotidesPf1N1B4_1180 and Pf1N1B4_1181 are separated by 61 nucleotidesPf1N1B4_1181 and Pf1N1B4_1182 overlap by 8 nucleotidesPf1N1B4_1182 and Pf1N1B4_1183 are separated by 7 nucleotides Pf1N1B4_1178 - DNA repair protein RecN, at 1,329,748 to 1,331,421 _1178 Pf1N1B4_1179 - Ferric uptake regulation protein FUR, at 1,331,497 to 1,331,901 _1179 Pf1N1B4_1180 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 1,331,999 to 1,332,526 _1180 Pf1N1B4_1181 - UPF0125 protein yfjF, at 1,332,588 to 1,332,902 _1181 Pf1N1B4_1182 - Putative oligoketide cyclase/dehydratase or lipid transport protein YfjG, at 1,332,895 to 1,333,329 _1182 Pf1N1B4_1183 - Sodium-dependent transporter family protein, at 1,333,337 to 1,334,629 _1183 Position (kb) 1331 1332 1333Strain fitness (log2 ratio) -3 -2 -1 0 1at 1331.039 kb on + strand, within Pf1N1B4_1178at 1331.064 kb on + strand, within Pf1N1B4_1178at 1331.264 kb on + strandat 1331.264 kb on + strandat 1331.264 kb on + strandat 1331.264 kb on + strandat 1331.264 kb on + strandat 1331.265 kb on - strandat 1331.265 kb on - strandat 1331.295 kb on + strandat 1331.295 kb on + strandat 1331.296 kb on - strandat 1331.296 kb on - strandat 1331.419 kb on + strandat 1331.498 kb on + strandat 1331.499 kb on - strandat 1331.499 kb on - strandat 1332.373 kb on + strand, within Pf1N1B4_1180at 1332.373 kb on + strand, within Pf1N1B4_1180at 1332.524 kb on + strandat 1332.525 kb on - strandat 1332.525 kb on - strandat 1332.540 kb on - strandat 1332.792 kb on + strand, within Pf1N1B4_1181at 1332.847 kb on - strand, within Pf1N1B4_1181at 1332.868 kb on + strandat 1332.868 kb on + strand, within Pf1N1B4_1181at 1332.868 kb on + strand, within Pf1N1B4_1181at 1332.869 kb on - strand, within Pf1N1B4_1181at 1332.869 kb on - strand, within Pf1N1B4_1181at 1332.869 kb on - strand, within Pf1N1B4_1181at 1332.869 kb on - strand, within Pf1N1B4_1181at 1332.869 kb on - strand, within Pf1N1B4_1181at 1333.339 kb on - strandat 1333.339 kb on - strandat 1333.339 kb on - strandat 1333.339 kb on - strandat 1333.339 kb on - strandat 1333.390 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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1,331,039 + Pf1N1B4_1178 0.77 -2.2
1,331,064 + Pf1N1B4_1178 0.79 -2.0
1,331,264 + -0.1
1,331,264 + -0.8
1,331,264 + -1.4
1,331,264 + -1.7
1,331,264 + -0.4
1,331,265 - -2.4
1,331,265 - -0.8
1,331,295 + -0.6
1,331,295 + -0.8
1,331,296 - -1.4
1,331,296 - +1.2
1,331,419 + -1.8
1,331,498 + -0.2
1,331,499 - +0.2
1,331,499 - +0.7
1,332,373 + Pf1N1B4_1180 0.71 -2.7
1,332,373 + Pf1N1B4_1180 0.71 -3.3
1,332,524 + +0.7
1,332,525 - +0.4
1,332,525 - -0.3
1,332,540 - -2.2
1,332,792 + Pf1N1B4_1181 0.65 +0.2
1,332,847 - Pf1N1B4_1181 0.82 -0.8
1,332,868 + +1.7
1,332,868 + Pf1N1B4_1181 0.89 -1.4
1,332,868 + Pf1N1B4_1181 0.89 +0.2
1,332,869 - Pf1N1B4_1181 0.89 -0.5
1,332,869 - Pf1N1B4_1181 0.89 -0.8
1,332,869 - Pf1N1B4_1181 0.89 -0.8
1,332,869 - Pf1N1B4_1181 0.89 +0.2
1,332,869 - Pf1N1B4_1181 0.89 -0.1
1,333,339 - +1.2
1,333,339 - -0.1
1,333,339 - -0.1
1,333,339 - -0.1
1,333,339 - -2.7
1,333,390 - -1.1

Or see this region's nucleotide sequence