Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotides Pf1N1B4_1134 and Pf1N1B4_1135 are separated by 179 nucleotides Pf1N1B4_1135 and Pf1N1B4_1136 are separated by 134 nucleotides
Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155
_1132
Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821
_1133
Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975
_1134
Pf1N1B4_1135 - FIG00960055: hypothetical protein, at 1,273,155 to 1,273,307
_1135
Pf1N1B4_1136 - Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19), at 1,273,442 to 1,274,605
_1136
Position (kb)
1271
1272
1273 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.854 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.896 kb on - strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.955 kb on + strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.956 kb on - strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.967 kb on + strand, within Pf1N1B4_1132 at 1270.998 kb on - strand, within Pf1N1B4_1132 at 1271.396 kb on + strand, within Pf1N1B4_1133 at 1271.397 kb on - strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.483 kb on + strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.484 kb on - strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.679 kb on + strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1271.680 kb on - strand, within Pf1N1B4_1133 at 1272.054 kb on + strand at 1272.054 kb on + strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.055 kb on - strand, within Pf1N1B4_1134 at 1272.064 kb on + strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.065 kb on - strand, within Pf1N1B4_1134 at 1272.211 kb on + strand, within Pf1N1B4_1134 at 1272.556 kb on - strand, within Pf1N1B4_1134 at 1272.667 kb on + strand, within Pf1N1B4_1134 at 1272.706 kb on + strand, within Pf1N1B4_1134 at 1272.754 kb on + strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.755 kb on - strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.784 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.797 kb on + strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.798 kb on - strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.801 kb on + strand, within Pf1N1B4_1134 at 1272.802 kb on - strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.820 kb on + strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134 at 1272.821 kb on - strand, within Pf1N1B4_1134 at 1272.904 kb on + strand at 1273.021 kb on - strand at 1273.021 kb on - strand at 1273.036 kb on - strand at 1273.111 kb on - strand at 1273.120 kb on - strand at 1273.151 kb on + strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.152 kb on - strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.159 kb on + strand at 1273.160 kb on - strand at 1273.160 kb on - strand at 1273.160 kb on - strand at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.283 kb on - strand, within Pf1N1B4_1135 at 1273.362 kb on + strand at 1273.362 kb on + strand at 1273.412 kb on - strand at 1273.464 kb on - strand at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.599 kb on - strand, within Pf1N1B4_1136 at 1273.601 kb on + strand, within Pf1N1B4_1136 at 1273.602 kb on - strand, within Pf1N1B4_1136 at 1273.682 kb on + strand, within Pf1N1B4_1136 at 1273.683 kb on - strand, within Pf1N1B4_1136 at 1273.770 kb on - strand, within Pf1N1B4_1136 at 1273.776 kb on - strand, within Pf1N1B4_1136 at 1273.796 kb on + strand, within Pf1N1B4_1136 at 1273.796 kb on + strand, within Pf1N1B4_1136 at 1273.797 kb on - strand, within Pf1N1B4_1136 at 1273.859 kb on + strand, within Pf1N1B4_1136 at 1273.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,270,854 - Pf1N1B4_1132 0.66 -0.6 1,270,854 - Pf1N1B4_1132 0.66 -1.8 1,270,896 - +1.7 1,270,896 - Pf1N1B4_1132 0.71 +0.7 1,270,896 - Pf1N1B4_1132 0.71 +1.5 1,270,955 + Pf1N1B4_1132 0.78 +0.4 1,270,955 + Pf1N1B4_1132 0.78 +1.2 1,270,955 + Pf1N1B4_1132 0.78 -1.8 1,270,955 + Pf1N1B4_1132 0.78 +0.2 1,270,955 + -0.8 1,270,955 + Pf1N1B4_1132 0.78 +1.0 1,270,955 + Pf1N1B4_1132 0.78 -2.2 1,270,955 + Pf1N1B4_1132 0.78 +0.2 1,270,955 + Pf1N1B4_1132 0.78 -3.0 1,270,956 - Pf1N1B4_1132 0.78 -0.9 1,270,956 - Pf1N1B4_1132 0.78 +1.6 1,270,956 - Pf1N1B4_1132 0.78 +1.2 1,270,956 - Pf1N1B4_1132 0.78 +0.9 1,270,967 + Pf1N1B4_1132 0.79 +1.4 1,270,967 + Pf1N1B4_1132 0.79 -1.7 1,270,967 + Pf1N1B4_1132 0.79 -0.8 1,270,998 - Pf1N1B4_1132 0.82 -0.2 1,271,396 + Pf1N1B4_1133 0.35 -0.5 1,271,397 - Pf1N1B4_1133 0.35 -1.5 1,271,483 + Pf1N1B4_1133 0.48 -2.0 1,271,483 + +0.2 1,271,483 + Pf1N1B4_1133 0.48 +2.0 1,271,483 + Pf1N1B4_1133 0.48 -2.4 1,271,483 + Pf1N1B4_1133 0.48 +0.3 1,271,484 - Pf1N1B4_1133 0.48 -0.0 1,271,484 - Pf1N1B4_1133 0.48 -1.4 1,271,484 - Pf1N1B4_1133 0.48 -0.6 1,271,484 - Pf1N1B4_1133 0.48 +2.2 1,271,484 - Pf1N1B4_1133 0.48 -0.2 1,271,484 - Pf1N1B4_1133 0.48 +1.1 1,271,679 + Pf1N1B4_1133 0.78 -1.2 1,271,679 + Pf1N1B4_1133 0.78 +0.7 1,271,680 - Pf1N1B4_1133 0.78 +0.2 1,271,680 - Pf1N1B4_1133 0.78 -0.6 1,271,680 - Pf1N1B4_1133 0.78 -0.8 1,272,054 + +0.7 1,272,054 + Pf1N1B4_1134 0.20 -2.4 1,272,055 - Pf1N1B4_1134 0.20 +3.2 1,272,055 - Pf1N1B4_1134 0.20 -0.8 1,272,064 + Pf1N1B4_1134 0.21 -1.4 1,272,065 - Pf1N1B4_1134 0.21 -0.4 1,272,065 - Pf1N1B4_1134 0.21 +1.0 1,272,211 + Pf1N1B4_1134 0.34 +0.2 1,272,556 - Pf1N1B4_1134 0.64 -2.2 1,272,667 + Pf1N1B4_1134 0.73 -0.8 1,272,706 + Pf1N1B4_1134 0.77 +1.2 1,272,754 + Pf1N1B4_1134 0.81 +0.2 1,272,755 - Pf1N1B4_1134 0.81 -1.0 1,272,755 - Pf1N1B4_1134 0.81 +0.8 1,272,784 + Pf1N1B4_1134 0.83 +1.7 1,272,784 + Pf1N1B4_1134 0.83 -1.8 1,272,797 + Pf1N1B4_1134 0.85 -0.4 1,272,797 + Pf1N1B4_1134 0.85 -0.3 1,272,797 + Pf1N1B4_1134 0.85 -0.0 1,272,797 + Pf1N1B4_1134 0.85 +0.7 1,272,797 + Pf1N1B4_1134 0.85 +0.7 1,272,797 + Pf1N1B4_1134 0.85 -2.2 1,272,797 + Pf1N1B4_1134 0.85 +0.9 1,272,797 + Pf1N1B4_1134 0.85 -0.1 1,272,797 + Pf1N1B4_1134 0.85 -0.4 1,272,797 + Pf1N1B4_1134 0.85 -0.8 1,272,797 + Pf1N1B4_1134 0.85 -0.4 1,272,798 - Pf1N1B4_1134 0.85 -1.4 1,272,798 - Pf1N1B4_1134 0.85 +0.5 1,272,798 - Pf1N1B4_1134 0.85 -1.3 1,272,798 - Pf1N1B4_1134 0.85 +0.6 1,272,798 - Pf1N1B4_1134 0.85 -0.3 1,272,798 - Pf1N1B4_1134 0.85 -0.3 1,272,798 - Pf1N1B4_1134 0.85 -2.2 1,272,801 + Pf1N1B4_1134 0.85 -1.2 1,272,801 + Pf1N1B4_1134 0.85 +0.6 1,272,801 + Pf1N1B4_1134 0.85 -0.4 1,272,801 + Pf1N1B4_1134 0.85 -0.8 1,272,801 + Pf1N1B4_1134 0.85 -0.8 1,272,802 - Pf1N1B4_1134 0.85 -0.8 1,272,820 + Pf1N1B4_1134 0.87 -0.8 1,272,820 + Pf1N1B4_1134 0.87 +0.2 1,272,820 + Pf1N1B4_1134 0.87 -0.3 1,272,820 + Pf1N1B4_1134 0.87 -0.4 1,272,821 - Pf1N1B4_1134 0.87 +1.7 1,272,821 - Pf1N1B4_1134 0.87 +1.6 1,272,904 + +2.7 1,273,021 - +1.7 1,273,021 - -0.4 1,273,036 - +0.2 1,273,111 - +1.7 1,273,120 - +0.2 1,273,151 + +1.1 1,273,152 - +0.6 1,273,152 - -0.2 1,273,152 - -0.1 1,273,152 - +1.0 1,273,152 - -0.4 1,273,152 - -0.8 1,273,159 + +0.2 1,273,159 + +1.3 1,273,159 + +0.2 1,273,159 + -0.4 1,273,160 - +0.4 1,273,160 - +0.9 1,273,160 - +2.2 1,273,283 - Pf1N1B4_1135 0.84 +0.3 1,273,283 - Pf1N1B4_1135 0.84 -1.4 1,273,283 - Pf1N1B4_1135 0.84 +2.2 1,273,283 - Pf1N1B4_1135 0.84 +1.2 1,273,283 - Pf1N1B4_1135 0.84 +1.5 1,273,362 + -0.6 1,273,362 + -1.9 1,273,412 - -0.9 1,273,464 - +0.4 1,273,599 - Pf1N1B4_1136 0.13 -0.4 1,273,599 - Pf1N1B4_1136 0.13 -0.8 1,273,599 - Pf1N1B4_1136 0.13 -0.1 1,273,599 - Pf1N1B4_1136 0.13 +3.6 1,273,601 + Pf1N1B4_1136 0.14 +0.5 1,273,602 - Pf1N1B4_1136 0.14 +0.6 1,273,682 + Pf1N1B4_1136 0.21 +1.7 1,273,683 - Pf1N1B4_1136 0.21 +0.1 1,273,770 - Pf1N1B4_1136 0.28 -1.9 1,273,776 - Pf1N1B4_1136 0.29 -1.1 1,273,796 + Pf1N1B4_1136 0.30 +0.3 1,273,796 + Pf1N1B4_1136 0.30 -2.9 1,273,797 - Pf1N1B4_1136 0.30 -2.2 1,273,859 + Pf1N1B4_1136 0.36 +0.6 1,273,860 - +0.1
Or see this region's nucleotide sequence