Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1104 and Pf1N1B4_1105 overlap by 4 nucleotides Pf1N1B4_1105 and Pf1N1B4_1106 are separated by 155 nucleotides
Pf1N1B4_1104 - K+-dependent Na+/Ca+ exchanger related-protein, at 1,237,196 to 1,238,257
_1104
Pf1N1B4_1105 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 1,238,254 to 1,238,634
_1105
Pf1N1B4_1106 - membrane protein, putative, at 1,238,790 to 1,239,731
_1106
Position (kb)
1238
1239 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1237.574 kb on - strand, within Pf1N1B4_1104 at 1237.574 kb on - strand, within Pf1N1B4_1104 at 1237.598 kb on + strand, within Pf1N1B4_1104 at 1237.599 kb on - strand, within Pf1N1B4_1104 at 1237.599 kb on - strand, within Pf1N1B4_1104 at 1237.612 kb on - strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.893 kb on + strand, within Pf1N1B4_1104 at 1237.894 kb on - strand, within Pf1N1B4_1104 at 1237.930 kb on - strand, within Pf1N1B4_1104 at 1237.930 kb on - strand, within Pf1N1B4_1104 at 1238.040 kb on - strand, within Pf1N1B4_1104 at 1238.040 kb on - strand, within Pf1N1B4_1104 at 1238.090 kb on + strand, within Pf1N1B4_1104 at 1238.090 kb on + strand, within Pf1N1B4_1104 at 1238.099 kb on + strand, within Pf1N1B4_1104 at 1238.100 kb on - strand at 1238.100 kb on - strand, within Pf1N1B4_1104 at 1238.266 kb on - strand at 1238.266 kb on - strand at 1238.557 kb on + strand at 1238.557 kb on + strand, within Pf1N1B4_1105 at 1238.557 kb on + strand, within Pf1N1B4_1105 at 1238.557 kb on + strand, within Pf1N1B4_1105 at 1238.558 kb on - strand, within Pf1N1B4_1105 at 1238.558 kb on - strand, within Pf1N1B4_1105 at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.613 kb on + strand at 1238.614 kb on - strand at 1238.614 kb on - strand at 1238.614 kb on - strand at 1238.617 kb on + strand at 1238.617 kb on + strand at 1238.617 kb on + strand at 1238.618 kb on - strand at 1238.632 kb on + strand at 1238.632 kb on + strand at 1238.633 kb on - strand at 1238.633 kb on - strand at 1238.709 kb on + strand at 1238.709 kb on + strand at 1238.709 kb on + strand at 1238.710 kb on - strand at 1238.718 kb on + strand at 1238.792 kb on - strand at 1238.859 kb on - strand at 1238.947 kb on + strand at 1238.947 kb on + strand, within Pf1N1B4_1106 at 1239.185 kb on - strand, within Pf1N1B4_1106 at 1239.185 kb on - strand, within Pf1N1B4_1106 at 1239.249 kb on - strand, within Pf1N1B4_1106 at 1239.249 kb on - strand, within Pf1N1B4_1106 at 1239.249 kb on - strand, within Pf1N1B4_1106 at 1239.249 kb on - strand, within Pf1N1B4_1106 at 1239.313 kb on + strand, within Pf1N1B4_1106 at 1239.314 kb on - strand, within Pf1N1B4_1106 at 1239.370 kb on + strand, within Pf1N1B4_1106 at 1239.370 kb on + strand, within Pf1N1B4_1106 at 1239.370 kb on + strand, within Pf1N1B4_1106 at 1239.370 kb on + strand at 1239.371 kb on - strand, within Pf1N1B4_1106 at 1239.433 kb on + strand, within Pf1N1B4_1106 at 1239.434 kb on - strand, within Pf1N1B4_1106 at 1239.434 kb on - strand, within Pf1N1B4_1106 at 1239.434 kb on - strand, within Pf1N1B4_1106 at 1239.434 kb on - strand, within Pf1N1B4_1106 at 1239.517 kb on + strand, within Pf1N1B4_1106 at 1239.517 kb on + strand, within Pf1N1B4_1106 at 1239.517 kb on + strand, within Pf1N1B4_1106 at 1239.517 kb on + strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.518 kb on - strand, within Pf1N1B4_1106 at 1239.621 kb on - strand, within Pf1N1B4_1106
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,237,574 - Pf1N1B4_1104 0.36 +2.3 1,237,574 - Pf1N1B4_1104 0.36 +1.3 1,237,598 + Pf1N1B4_1104 0.38 +0.1 1,237,599 - Pf1N1B4_1104 0.38 +1.1 1,237,599 - Pf1N1B4_1104 0.38 +3.0 1,237,612 - Pf1N1B4_1104 0.39 +0.1 1,237,893 + Pf1N1B4_1104 0.66 -1.1 1,237,893 + Pf1N1B4_1104 0.66 +0.1 1,237,893 + Pf1N1B4_1104 0.66 -1.3 1,237,893 + Pf1N1B4_1104 0.66 -1.6 1,237,894 - Pf1N1B4_1104 0.66 +0.9 1,237,930 - Pf1N1B4_1104 0.69 +0.7 1,237,930 - Pf1N1B4_1104 0.69 +0.2 1,238,040 - Pf1N1B4_1104 0.79 +0.8 1,238,040 - Pf1N1B4_1104 0.79 -0.1 1,238,090 + Pf1N1B4_1104 0.84 +0.0 1,238,090 + Pf1N1B4_1104 0.84 +1.4 1,238,099 + Pf1N1B4_1104 0.85 +0.7 1,238,100 - +2.5 1,238,100 - Pf1N1B4_1104 0.85 -0.5 1,238,266 - -0.1 1,238,266 - -1.8 1,238,557 + +2.9 1,238,557 + Pf1N1B4_1105 0.80 -1.4 1,238,557 + Pf1N1B4_1105 0.80 +0.7 1,238,557 + Pf1N1B4_1105 0.80 -1.7 1,238,558 - Pf1N1B4_1105 0.80 -0.9 1,238,558 - Pf1N1B4_1105 0.80 -1.4 1,238,613 + -0.9 1,238,613 + +0.4 1,238,613 + +1.1 1,238,613 + -1.0 1,238,613 + -0.1 1,238,613 + -0.9 1,238,613 + -0.1 1,238,614 - -0.4 1,238,614 - -0.2 1,238,614 - -1.2 1,238,617 + +1.5 1,238,617 + +0.6 1,238,617 + +0.9 1,238,618 - +0.6 1,238,632 + -0.9 1,238,632 + -0.5 1,238,633 - -1.2 1,238,633 - -1.4 1,238,709 + +1.4 1,238,709 + +0.7 1,238,709 + +0.1 1,238,710 - -0.1 1,238,718 + +0.4 1,238,792 - +0.1 1,238,859 - -0.9 1,238,947 + -0.9 1,238,947 + Pf1N1B4_1106 0.17 +0.1 1,239,185 - Pf1N1B4_1106 0.42 -1.9 1,239,185 - Pf1N1B4_1106 0.42 +0.4 1,239,249 - Pf1N1B4_1106 0.49 -0.3 1,239,249 - Pf1N1B4_1106 0.49 +0.6 1,239,249 - Pf1N1B4_1106 0.49 -1.4 1,239,249 - Pf1N1B4_1106 0.49 +0.9 1,239,313 + Pf1N1B4_1106 0.56 +0.5 1,239,314 - Pf1N1B4_1106 0.56 +0.7 1,239,370 + Pf1N1B4_1106 0.62 -0.1 1,239,370 + Pf1N1B4_1106 0.62 -1.4 1,239,370 + Pf1N1B4_1106 0.62 -1.2 1,239,370 + -1.4 1,239,371 - Pf1N1B4_1106 0.62 -1.9 1,239,433 + Pf1N1B4_1106 0.68 +0.1 1,239,434 - Pf1N1B4_1106 0.68 -0.4 1,239,434 - Pf1N1B4_1106 0.68 -2.2 1,239,434 - Pf1N1B4_1106 0.68 -0.2 1,239,434 - Pf1N1B4_1106 0.68 -0.3 1,239,517 + Pf1N1B4_1106 0.77 -1.1 1,239,517 + Pf1N1B4_1106 0.77 -1.7 1,239,517 + Pf1N1B4_1106 0.77 +3.5 1,239,517 + Pf1N1B4_1106 0.77 +0.1 1,239,518 - Pf1N1B4_1106 0.77 -0.9 1,239,518 - Pf1N1B4_1106 0.77 -0.9 1,239,518 - Pf1N1B4_1106 0.77 -0.4 1,239,518 - Pf1N1B4_1106 0.77 +0.9 1,239,518 - Pf1N1B4_1106 0.77 -1.9 1,239,518 - Pf1N1B4_1106 0.77 +0.1 1,239,518 - Pf1N1B4_1106 0.77 +0.1 1,239,621 - Pf1N1B4_1106 0.88 -0.9
Or see this region's nucleotide sequence