Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1066 and Pf1N1B4_1067 are separated by 126 nucleotides Pf1N1B4_1067 and Pf1N1B4_1068 are separated by 128 nucleotides Pf1N1B4_1068 and Pf1N1B4_1069 are separated by 22 nucleotides Pf1N1B4_1069 and Pf1N1B4_1070 are separated by 115 nucleotides Pf1N1B4_1070 and Pf1N1B4_1071 overlap by 4 nucleotides
Pf1N1B4_1066 - Histidinol dehydrogenase (EC 1.1.1.23), at 1,201,450 to 1,202,775
_1066
Pf1N1B4_1067 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 1,202,902 to 1,203,537
_1067
Pf1N1B4_1068 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 1,203,666 to 1,204,931
_1068
Pf1N1B4_1069 - YrbA protein, at 1,204,954 to 1,205,193
_1069
Pf1N1B4_1070 - Uncharacterized protein YrbB, at 1,205,309 to 1,205,614
_1070
Pf1N1B4_1071 - Uncharacterized ABC transporter, auxiliary component YrbC, at 1,205,611 to 1,206,264
_1071
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.790 kb on - strand at 1202.821 kb on - strand at 1202.821 kb on - strand at 1202.821 kb on - strand at 1203.128 kb on + strand, within Pf1N1B4_1067 at 1203.129 kb on - strand, within Pf1N1B4_1067 at 1203.167 kb on + strand at 1203.171 kb on - strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.290 kb on + strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.291 kb on - strand, within Pf1N1B4_1067 at 1203.309 kb on - strand, within Pf1N1B4_1067 at 1203.365 kb on + strand, within Pf1N1B4_1067 at 1203.365 kb on + strand at 1203.365 kb on + strand, within Pf1N1B4_1067 at 1203.366 kb on - strand, within Pf1N1B4_1067 at 1203.366 kb on - strand, within Pf1N1B4_1067 at 1203.562 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.594 kb on + strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.595 kb on - strand at 1203.667 kb on + strand at 1204.996 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.046 kb on - strand, within Pf1N1B4_1069 at 1205.095 kb on - strand, within Pf1N1B4_1069 at 1205.138 kb on - strand, within Pf1N1B4_1069 at 1205.183 kb on - strand at 1205.238 kb on - strand at 1205.238 kb on - strand at 1205.238 kb on - strand at 1205.297 kb on + strand at 1205.299 kb on + strand at 1205.299 kb on + strand at 1205.299 kb on + strand at 1205.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,202,790 - -0.2 1,202,790 - +0.9 1,202,790 - +1.7 1,202,790 - -1.5 1,202,821 - +1.4 1,202,821 - +1.0 1,202,821 - -1.9 1,203,128 + Pf1N1B4_1067 0.36 -1.5 1,203,129 - Pf1N1B4_1067 0.36 +1.7 1,203,167 + +1.7 1,203,171 - Pf1N1B4_1067 0.42 -0.6 1,203,290 + Pf1N1B4_1067 0.61 +0.5 1,203,290 + Pf1N1B4_1067 0.61 -0.9 1,203,290 + Pf1N1B4_1067 0.61 -0.0 1,203,291 - Pf1N1B4_1067 0.61 -0.6 1,203,291 - Pf1N1B4_1067 0.61 +0.9 1,203,291 - Pf1N1B4_1067 0.61 +0.1 1,203,291 - Pf1N1B4_1067 0.61 +0.3 1,203,291 - Pf1N1B4_1067 0.61 -0.0 1,203,291 - Pf1N1B4_1067 0.61 -0.6 1,203,309 - Pf1N1B4_1067 0.64 -2.5 1,203,365 + Pf1N1B4_1067 0.73 +0.8 1,203,365 + +0.1 1,203,365 + Pf1N1B4_1067 0.73 -0.0 1,203,366 - Pf1N1B4_1067 0.73 +4.0 1,203,366 - Pf1N1B4_1067 0.73 -1.1 1,203,562 + -0.9 1,203,594 + -0.2 1,203,594 + -0.5 1,203,594 + +0.1 1,203,594 + +2.4 1,203,595 - -0.3 1,203,595 - +0.1 1,203,595 - -0.4 1,203,595 - -0.9 1,203,595 - -0.9 1,203,595 - +0.9 1,203,667 + -2.2 1,204,996 - Pf1N1B4_1069 0.17 -1.6 1,205,046 - Pf1N1B4_1069 0.38 -1.9 1,205,046 - Pf1N1B4_1069 0.38 -0.9 1,205,046 - Pf1N1B4_1069 0.38 -0.6 1,205,046 - Pf1N1B4_1069 0.38 +0.1 1,205,046 - Pf1N1B4_1069 0.38 -1.9 1,205,046 - Pf1N1B4_1069 0.38 -0.6 1,205,046 - Pf1N1B4_1069 0.38 +0.1 1,205,046 - Pf1N1B4_1069 0.38 -0.2 1,205,046 - Pf1N1B4_1069 0.38 -2.2 1,205,046 - Pf1N1B4_1069 0.38 +1.1 1,205,046 - Pf1N1B4_1069 0.38 -0.5 1,205,046 - Pf1N1B4_1069 0.38 +0.1 1,205,046 - Pf1N1B4_1069 0.38 -2.3 1,205,046 - Pf1N1B4_1069 0.38 -1.9 1,205,046 - Pf1N1B4_1069 0.38 -2.2 1,205,046 - Pf1N1B4_1069 0.38 +0.1 1,205,046 - Pf1N1B4_1069 0.38 -1.9 1,205,046 - Pf1N1B4_1069 0.38 -0.7 1,205,046 - Pf1N1B4_1069 0.38 -0.2 1,205,046 - Pf1N1B4_1069 0.38 -0.6 1,205,046 - Pf1N1B4_1069 0.38 -0.6 1,205,095 - Pf1N1B4_1069 0.59 -3.0 1,205,138 - Pf1N1B4_1069 0.77 +0.1 1,205,183 - +0.1 1,205,238 - -0.9 1,205,238 - +0.7 1,205,238 - -2.9 1,205,297 + -2.0 1,205,299 + -0.9 1,205,299 + +1.1 1,205,299 + +1.8 1,205,300 - +1.7
Or see this region's nucleotide sequence