Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1004 and Pf1N1B4_1005 are separated by 336 nucleotides Pf1N1B4_1005 and Pf1N1B4_1006 are separated by 21 nucleotides
Pf1N1B4_1004 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 1,138,974 to 1,139,948
_1004
Pf1N1B4_1005 - probable membrane protein YPO0899, at 1,140,285 to 1,140,650
_1005
Pf1N1B4_1006 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,140,672 to 1,141,574
_1006
Position (kb)
1140
1141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.306 kb on + strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.307 kb on - strand, within Pf1N1B4_1004 at 1139.489 kb on + strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.490 kb on - strand, within Pf1N1B4_1004 at 1139.493 kb on + strand, within Pf1N1B4_1004 at 1139.760 kb on + strand, within Pf1N1B4_1004 at 1139.994 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.020 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.036 kb on - strand at 1140.084 kb on - strand at 1140.100 kb on + strand at 1140.203 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.229 kb on - strand at 1140.259 kb on - strand at 1140.259 kb on - strand at 1140.264 kb on + strand at 1140.312 kb on + strand at 1140.312 kb on + strand at 1140.313 kb on - strand at 1140.330 kb on - strand, within Pf1N1B4_1005 at 1140.415 kb on - strand, within Pf1N1B4_1005 at 1140.593 kb on + strand, within Pf1N1B4_1005 at 1140.841 kb on + strand at 1140.841 kb on + strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.842 kb on - strand, within Pf1N1B4_1006 at 1140.922 kb on + strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.923 kb on - strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1140.940 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.012 kb on + strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.013 kb on - strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.098 kb on + strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.099 kb on - strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.151 kb on + strand, within Pf1N1B4_1006 at 1141.152 kb on - strand at 1141.152 kb on - strand at 1141.331 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.407 kb on + strand, within Pf1N1B4_1006 at 1141.408 kb on - strand, within Pf1N1B4_1006 at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.554 kb on + strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.555 kb on - strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.558 kb on + strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand at 1141.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,139,306 + Pf1N1B4_1004 0.34 -0.4 1,139,306 + Pf1N1B4_1004 0.34 -2.2 1,139,307 - Pf1N1B4_1004 0.34 -0.6 1,139,307 - Pf1N1B4_1004 0.34 -2.1 1,139,307 - Pf1N1B4_1004 0.34 -1.2 1,139,489 + Pf1N1B4_1004 0.53 +0.1 1,139,490 - Pf1N1B4_1004 0.53 +1.1 1,139,490 - Pf1N1B4_1004 0.53 -0.9 1,139,493 + Pf1N1B4_1004 0.53 -0.9 1,139,760 + Pf1N1B4_1004 0.81 +0.1 1,139,994 - +0.8 1,140,020 - +0.1 1,140,020 - -1.9 1,140,020 - -1.0 1,140,020 - +0.2 1,140,036 - -2.5 1,140,036 - -2.2 1,140,036 - -1.5 1,140,084 - +2.4 1,140,100 + -1.9 1,140,203 - -0.5 1,140,229 - -0.2 1,140,229 - +1.2 1,140,229 - -1.7 1,140,259 - +0.1 1,140,259 - +0.1 1,140,264 + +0.1 1,140,312 + -2.5 1,140,312 + -1.5 1,140,313 - -0.1 1,140,330 - Pf1N1B4_1005 0.12 +0.5 1,140,415 - Pf1N1B4_1005 0.36 -0.4 1,140,593 + Pf1N1B4_1005 0.84 -1.5 1,140,841 + -0.6 1,140,841 + Pf1N1B4_1006 0.19 -0.3 1,140,842 - Pf1N1B4_1006 0.19 +0.1 1,140,842 - Pf1N1B4_1006 0.19 -2.7 1,140,922 + Pf1N1B4_1006 0.28 +0.4 1,140,923 - Pf1N1B4_1006 0.28 +0.1 1,140,923 - Pf1N1B4_1006 0.28 +0.1 1,140,923 - Pf1N1B4_1006 0.28 +0.3 1,140,940 + Pf1N1B4_1006 0.30 +0.5 1,140,940 + Pf1N1B4_1006 0.30 +0.1 1,140,940 + Pf1N1B4_1006 0.30 -0.3 1,140,940 + Pf1N1B4_1006 0.30 +0.7 1,141,012 + Pf1N1B4_1006 0.38 +0.4 1,141,012 + Pf1N1B4_1006 0.38 -0.6 1,141,013 - Pf1N1B4_1006 0.38 -0.2 1,141,013 - Pf1N1B4_1006 0.38 +1.1 1,141,098 + Pf1N1B4_1006 0.47 -2.5 1,141,098 + Pf1N1B4_1006 0.47 -1.5 1,141,098 + Pf1N1B4_1006 0.47 -0.3 1,141,099 - Pf1N1B4_1006 0.47 +1.3 1,141,099 - Pf1N1B4_1006 0.47 +0.3 1,141,151 + Pf1N1B4_1006 0.53 -0.6 1,141,151 + Pf1N1B4_1006 0.53 +0.1 1,141,151 + Pf1N1B4_1006 0.53 +0.7 1,141,152 - +0.8 1,141,152 - +1.1 1,141,331 + Pf1N1B4_1006 0.73 -0.6 1,141,407 + Pf1N1B4_1006 0.81 -0.4 1,141,407 + Pf1N1B4_1006 0.81 +0.1 1,141,407 + Pf1N1B4_1006 0.81 +0.8 1,141,407 + Pf1N1B4_1006 0.81 -0.8 1,141,407 + Pf1N1B4_1006 0.81 -1.7 1,141,408 - Pf1N1B4_1006 0.82 +1.1 1,141,554 + -0.7 1,141,554 + -0.4 1,141,554 + +0.9 1,141,555 - -1.0 1,141,555 - +1.3 1,141,555 - +1.3 1,141,555 - +1.1 1,141,555 - -0.9 1,141,555 - -0.5 1,141,558 + -1.5 1,141,558 + -1.5 1,141,558 + +0.4 1,141,558 + +1.5 1,141,558 + -0.6 1,141,558 + -2.5 1,141,558 + -1.9 1,141,559 - +0.1 1,141,559 - -0.3 1,141,559 - +1.1 1,141,559 - +1.3
Or see this region's nucleotide sequence