Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS20210

Experiment: Temperature shift 30_to_21

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_21
4,572,944	+				+0.5
4,572,944	+				-2.2
4,572,945	-				+0.3
4,572,947	+				+0.9
4,572,948	-				+0.3
4,572,948	-				-0.9
4,572,961	+				-0.9
4,572,962	-				+1.3
4,572,999	+				-0.5
4,572,999	+				+0.0
4,573,000	-				-0.1
4,573,000	-				+0.3
4,573,016	+				-1.2
4,573,054	+				-1.0
4,573,054	+				+0.3
4,573,054	+				+0.7
4,573,054	+				-0.5
4,573,054	+				+0.8
4,573,054	+				-1.1
4,573,054	+				+2.1
4,573,054	+				+1.7
4,573,054	+				-0.2
4,573,055	-				-0.2
4,573,055	-				-1.5
4,573,055	-				-0.9
4,573,055	-				-0.5
4,573,055	-				+0.7
4,573,055	-				+0.7
4,573,055	-				+0.1
4,573,055	-				-0.1
4,573,055	-				+0.2
4,573,055	-				-0.4
4,573,055	-				-0.2
4,573,055	-				-1.2
4,573,055	-				-1.8
4,573,069	-				-0.5
4,573,136	-		PFLU_RS30965	0.23	+0.4
4,573,136	-		PFLU_RS30965	0.23	-0.7
4,573,149	+		PFLU_RS30965	0.27	+1.9
4,573,163	+		PFLU_RS30965	0.31	-1.1
4,573,163	+		PFLU_RS30965	0.31	+0.8
4,573,164	-		PFLU_RS30965	0.32	-2.1
4,573,174	+		PFLU_RS30965	0.35	-0.9
4,573,197	+		PFLU_RS30965	0.42	+1.4
4,573,197	+		PFLU_RS30965	0.42	-1.9
4,573,197	+		PFLU_RS30965	0.42	+0.9
4,573,197	+		PFLU_RS30965	0.42	+1.5
4,573,198	-		PFLU_RS30965	0.42	+0.2
4,573,198	-		PFLU_RS30965	0.42	+0.2
4,573,208	-		PFLU_RS30965	0.45	-0.8
4,573,254	+		PFLU_RS30965	0.60	+0.9
4,573,254	+		PFLU_RS30965	0.60	+1.9
4,573,255	-		PFLU_RS30965	0.60	-0.4
4,573,255	-		PFLU_RS30965	0.60	+0.4
4,573,255	-		PFLU_RS30965	0.60	+1.5
4,573,255	-		PFLU_RS30965	0.60	-2.1
4,573,255	-		PFLU_RS30965	0.60	-0.2
4,573,255	-		PFLU_RS30965	0.60	+0.7
4,573,255	-		PFLU_RS30965	0.60	+0.1
4,573,255	-		PFLU_RS30965	0.60	+0.6
4,573,255	-		PFLU_RS30965	0.60	-0.1
4,573,255	-		PFLU_RS30965	0.60	-0.9
4,573,285	+		PFLU_RS30965	0.69	+0.6
4,573,285	+		PFLU_RS30965	0.69	+0.3
4,573,285	+		PFLU_RS30965	0.69	-0.1
4,573,285	+		PFLU_RS30965	0.69	+0.9
4,573,285	+		PFLU_RS30965	0.69	-1.1
4,573,285	+		PFLU_RS30965	0.69	-0.3
4,573,285	+		PFLU_RS30965	0.69	-1.9
4,573,285	+		PFLU_RS30965	0.69	-0.7
4,573,285	+		PFLU_RS30965	0.69	-2.2
4,573,285	+		PFLU_RS30965	0.69	+0.7
4,573,285	+		PFLU_RS30965	0.69	+0.7
4,573,285	+		PFLU_RS30965	0.69	-2.7
4,573,285	+		PFLU_RS30965	0.69	-2.2
4,573,285	+		PFLU_RS30965	0.69	+0.5
4,573,285	+		PFLU_RS30965	0.69	+1.1
4,573,286	-		PFLU_RS30965	0.69	-2.5
4,573,286	-		PFLU_RS30965	0.69	+0.7
4,573,286	-		PFLU_RS30965	0.69	+0.1
4,573,286	-		PFLU_RS30965	0.69	-0.1
4,573,286	-		PFLU_RS30965	0.69	-0.4
4,573,286	-		PFLU_RS30965	0.69	+0.9
4,573,286	-		PFLU_RS30965	0.69	-0.9
4,573,286	-		PFLU_RS30965	0.69	+0.3
4,573,286	-		PFLU_RS30965	0.69	-2.8
4,573,286	-		PFLU_RS30965	0.69	-1.2
4,573,286	-		PFLU_RS30965	0.69	+1.9
4,573,286	-		PFLU_RS30965	0.69	+0.6
4,573,286	-		PFLU_RS30965	0.69	+0.7
4,573,286	-		PFLU_RS30965	0.69	-0.9
4,573,286	-		PFLU_RS30965	0.69	-1.5
4,573,286	-		PFLU_RS30965	0.69	+0.0
4,573,286	-		PFLU_RS30965	0.69	+0.6
4,573,286	-		PFLU_RS30965	0.69	+0.3
4,573,286	-		PFLU_RS30965	0.69	+0.5
4,573,287	+		PFLU_RS30965	0.70	-1.0
4,573,288	-		PFLU_RS30965	0.70	-0.4
4,573,288	-		PFLU_RS30965	0.70	+0.9
4,573,350	-		PFLU_RS30965	0.89	-0.4
4,573,350	-		PFLU_RS30965	0.89	-0.1
4,573,351	+		PFLU_RS30965	0.90	-0.0
4,573,351	+		PFLU_RS30965	0.90	-0.7
4,573,352	-				-0.1
4,573,359	+				-0.6
4,573,359	+				-0.1
4,573,359	+				+0.6
4,573,359	+				+0.8
4,573,360	-				+0.9
4,573,360	-				+1.0
4,573,360	-				+0.6
4,573,382	+				-0.2
4,573,387	+				-1.4
4,573,388	-				-0.1
4,573,429	+				-1.2
4,573,429	+				-0.9
4,573,429	+				-1.1
4,573,429	+				+0.8
4,573,429	+				-0.5
4,573,429	+				-1.1
4,573,429	+				+1.5
4,573,430	-				-0.1
4,573,430	-				+0.8
4,573,430	-				+1.2
4,573,430	-				+1.2
4,573,430	-				+1.8
4,573,442	-				+0.9
4,573,545	+				+0.3
4,573,545	+				+0.1
4,573,545	+				+0.5
4,573,545	+				-0.7
4,573,545	+				-0.5
4,573,545	+				+0.1
4,573,545	+				+0.3
4,573,545	+				+0.9
4,573,546	-				+1.3
4,573,546	-				+0.9
4,573,546	-				+2.1
4,573,546	-				+1.1
4,573,546	-				-0.1
4,573,546	-				-0.2
4,573,546	-				-1.5
4,573,549	+				+1.7
4,573,549	+				+1.1
4,573,550	-				+0.4
4,573,550	-				+0.8
4,573,550	-				+2.1
4,573,550	-				+0.9
4,573,550	-				-0.7
4,573,580	+				-1.2
4,573,580	+				-0.2
4,573,622	+				+0.9
4,573,622	+				+0.4
4,573,622	+				-0.6
4,573,622	+				+0.2
4,573,622	+				+0.5
4,573,623	-				-0.2
4,573,643	+				+0.9
4,573,669	+				-0.9
4,573,685	+				-0.3
4,573,685	+				+0.3
4,573,685	+				+0.0
4,573,685	+				+0.6
4,573,685	+				+0.5
4,573,685	+				+2.5
4,573,685	+				+0.3
4,573,685	+				-0.0
4,573,686	-				-0.3
4,573,686	-				+1.7
4,573,686	-				+0.1
4,573,686	-				-1.9
4,573,686	-				+0.6
4,573,686	-				+0.1
4,573,700	+				+1.0
4,573,700	+				+0.8
4,573,701	-				+0.4
4,573,701	-				+0.9
4,573,718	-				+0.9
4,573,808	+				+0.3
4,573,823	-				+0.5
4,573,834	-				-0.1
4,573,867	+				+1.3
4,573,867	+				+0.4
4,573,867	+				+0.3
4,573,894	+				+2.2
4,573,895	-				+1.3
4,573,912	+				+0.1
4,573,913	-				-1.1
4,573,913	-				+0.3
4,573,943	+				+0.7
4,573,943	+				+0.8
4,573,944	-				+1.2
4,573,944	-				-1.7
4,573,973	+		PFLU_RS20210	0.21	-0.1
4,573,973	+		PFLU_RS20210	0.21	+0.2
4,573,973	+		PFLU_RS20210	0.21	+1.8
4,573,974	-		PFLU_RS20210	0.21	+0.9
4,573,974	-		PFLU_RS20210	0.21	+0.9
4,573,974	-		PFLU_RS20210	0.21	-0.9
4,573,974	-		PFLU_RS20210	0.21	-0.7
4,573,974	-		PFLU_RS20210	0.21	-0.8
4,573,974	-		PFLU_RS20210	0.21	+1.2
4,574,038	+		PFLU_RS20210	0.50	+0.4
4,574,115	+		PFLU_RS20210	0.85	+0.8
4,574,115	+		PFLU_RS20210	0.85	-0.4
4,574,115	+		PFLU_RS20210	0.85	+0.4
4,574,115	+		PFLU_RS20210	0.85	-0.1
4,574,115	+		PFLU_RS20210	0.85	+0.9
4,574,116	-		PFLU_RS20210	0.86	+0.3
4,574,116	-		PFLU_RS20210	0.86	+0.1
4,574,125	+				-2.1
4,574,125	+				-0.6
4,574,125	+				-1.6
4,574,125	+				-0.9
4,574,125	+				+0.7
4,574,125	+				+0.3
4,574,125	+				-0.9
4,574,125	+				-0.5
4,574,125	+				-0.1
4,574,126	-				-0.0
4,574,126	-				-0.2
4,574,126	-				-0.2
4,574,126	-				-0.2
4,574,126	-				+1.5
4,574,126	-				+0.3
4,574,144	+				-0.3
4,574,144	+				-1.0
4,574,144	+				+0.1
4,574,166	+				-0.4
4,574,166	+				-1.0
4,574,166	+				-0.2
4,574,166	+				+0.7
4,574,166	+				-0.5
4,574,167	-				-0.5
4,574,186	+				+0.1
4,574,187	-				+1.0
4,574,191	+				-0.1
4,574,191	+				+0.6
4,574,321	+		PFLU_RS20215	0.60	+0.0
4,574,321	+		PFLU_RS20215	0.60	+0.5
4,574,321	+		PFLU_RS20215	0.60	+0.6
4,574,321	+		PFLU_RS20215	0.60	-0.3
4,574,321	+		PFLU_RS20215	0.60	-1.4
4,574,322	-		PFLU_RS20215	0.60	+0.9
4,574,322	-		PFLU_RS20215	0.60	-0.3
4,574,322	-		PFLU_RS20215	0.60	+1.0
4,574,322	-		PFLU_RS20215	0.60	+1.5
4,574,322	-		PFLU_RS20215	0.60	+0.5
4,574,322	-		PFLU_RS20215	0.60	+0.5
4,574,322	-		PFLU_RS20215	0.60	-0.6
4,574,322	-		PFLU_RS20215	0.60	+0.9
4,574,341	+		PFLU_RS20215	0.67	-0.2
4,574,341	+		PFLU_RS20215	0.67	+0.5
4,574,341	+		PFLU_RS20215	0.67	+0.1
4,574,341	+		PFLU_RS20215	0.67	-0.0
4,574,341	+		PFLU_RS20215	0.67	-0.7
4,574,341	+		PFLU_RS20215	0.67	-0.6
4,574,341	+		PFLU_RS20215	0.67	+1.3
4,574,342	-		PFLU_RS20215	0.68	+1.3
4,574,342	-		PFLU_RS20215	0.68	-0.1
4,574,342	-		PFLU_RS20215	0.68	-0.7
4,574,342	-		PFLU_RS20215	0.68	+0.9
4,574,342	-		PFLU_RS20215	0.68	+0.5
4,574,342	-		PFLU_RS20215	0.68	-1.2
4,574,342	-		PFLU_RS20215	0.68	-0.5
4,574,342	-		PFLU_RS20215	0.68	-0.4
4,574,403	+				+0.6
4,574,403	+				-1.7
4,574,403	+				+1.1
4,574,403	+				+0.5
4,574,403	+				+0.6
4,574,403	+				-0.2
4,574,403	+				-0.1
4,574,403	+				+0.9
4,574,403	+				+1.2
4,574,403	+				-0.9
4,574,403	+				-0.8
4,574,403	+				+0.4
4,574,403	+				+0.5
4,574,403	+				+1.2
4,574,404	-				+2.9
4,574,404	-				-1.8
4,574,404	-				+0.9
4,574,404	-				+0.6
4,574,404	-				-0.4
4,574,404	-				+0.1
4,574,404	-				+0.1
4,574,404	-				-0.7
4,574,432	+				-0.9
4,574,432	+				-0.7
4,574,432	+				+0.9
4,574,432	+				-1.7
4,574,432	+				-1.3
4,574,432	+				-0.3
4,574,433	-				+1.7
4,574,433	-				-0.1
4,574,437	+				+0.5
4,574,438	-				+0.0
4,574,455	+				+0.3
4,574,455	+				-0.5
4,574,455	+				+1.1
4,574,455	+				+0.6
4,574,455	+				+0.2
4,574,455	+				-0.7
4,574,455	+				+0.7
4,574,456	-				-2.1
4,574,456	-				+0.3
4,574,456	-				+0.3
4,574,459	+				+0.3
4,574,459	+				-1.1
4,574,460	-				+0.2
4,574,460	-				-0.1
4,574,460	-				+0.2
4,574,460	-				+0.1
4,574,460	-				+0.5
4,574,473	-				+3.2
4,574,502	+				-0.8
4,574,502	+				+1.1
4,574,502	+				+0.2
4,574,502	+				-0.5
4,574,503	-				+0.2
4,574,507	+				-0.6
4,574,507	+				+0.3
4,574,507	+				+1.2
4,574,507	+				+1.1
4,574,507	+				+0.1
4,574,507	+				-0.2
4,574,507	+				-0.7
4,574,507	+				-0.1
4,574,507	+				+0.9
4,574,507	+				+0.1
4,574,507	+				-0.7
4,574,507	+				+0.5
4,574,508	-				-0.5
4,574,508	-				+0.5
4,574,508	-				-0.3
4,574,508	-				-2.2
4,574,508	-				-0.2
4,574,508	-				+1.3
4,574,508	-				+0.2
4,574,508	-				-1.2
4,574,508	-				-1.1
4,574,508	-				+1.7
4,574,508	-				+2.1
4,574,508	-				-1.4
4,574,508	-				-0.3
4,574,508	-				-1.1
4,574,508	-				-2.1
4,574,511	+		PFLU_RS30970	0.11	-0.5
4,574,511	+		PFLU_RS30970	0.11	-0.4
4,574,511	+		PFLU_RS30970	0.11	-0.8
4,574,512	-		PFLU_RS30970	0.11	-0.1
4,574,512	-		PFLU_RS30970	0.11	+0.0
4,574,512	-		PFLU_RS30970	0.11	-0.5
4,574,512	-		PFLU_RS30970	0.11	-0.3
4,574,512	-		PFLU_RS30970	0.11	+1.9
4,574,512	-		PFLU_RS30970	0.11	+0.8
4,574,518	+		PFLU_RS30970	0.13	-3.4
4,574,518	+		PFLU_RS30970	0.13	+0.5
4,574,519	-		PFLU_RS30970	0.14	-0.9
4,574,519	-		PFLU_RS30970	0.14	+0.7
4,574,519	-		PFLU_RS30970	0.14	-1.9
4,574,519	-		PFLU_RS30970	0.14	-1.8
4,574,520	+		PFLU_RS30970	0.14	+0.2
4,574,520	+		PFLU_RS30970	0.14	+0.3
4,574,520	+		PFLU_RS30970	0.14	-0.1
4,574,521	-		PFLU_RS30970	0.15	+0.3
4,574,521	-		PFLU_RS30970	0.15	+0.2
4,574,521	-		PFLU_RS30970	0.15	-0.9
4,574,521	-		PFLU_RS30970	0.15	-0.5
4,574,521	-		PFLU_RS30970	0.15	+0.3
4,574,521	-		PFLU_RS30970	0.15	-0.9
4,574,590	+		PFLU_RS30970	0.41	+0.8
4,574,591	-		PFLU_RS30970	0.41	-0.9
4,574,612	-		PFLU_RS30970	0.49	-0.2
4,574,626	+		PFLU_RS30970	0.55	+0.8
4,574,626	+		PFLU_RS30970	0.55	+1.0
4,574,627	-		PFLU_RS30970	0.55	+0.2
4,574,627	-		PFLU_RS30970	0.55	-2.8
4,574,627	-		PFLU_RS30970	0.55	+0.7
4,574,627	-		PFLU_RS30970	0.55	-1.5
4,574,627	-		PFLU_RS30970	0.55	+1.8
4,574,627	-		PFLU_RS30970	0.55	-0.1
4,574,627	-		PFLU_RS30970	0.55	+0.3
4,574,672	-		PFLU_RS30970	0.72	+0.2
4,574,686	+		PFLU_RS30970	0.78	-0.5
4,574,686	+		PFLU_RS30970	0.78	-0.4
4,574,687	-		PFLU_RS30970	0.78	-0.3
4,574,712	+		PFLU_RS30970	0.88	+0.2
4,574,712	+		PFLU_RS30970	0.88	-1.7
4,574,719	+				+0.7
4,574,751	+				-0.2
4,574,767	+				+0.3
4,574,767	+				-0.1
4,574,768	-				+0.2
4,574,768	-				+2.2
4,574,805	+				-0.7
4,574,805	+				-0.4
4,574,805	+				-0.7
4,574,805	+				+0.9
4,574,806	-				-2.7
4,574,806	-				+1.3
4,574,806	-				-0.7
4,574,806	-				-0.1
4,574,858	+				+0.8
4,574,858	+				+0.2
4,574,858	+				-0.2
4,574,858	+				+0.3
4,574,858	+				-0.7
4,574,858	+				+0.3
4,574,858	+				+0.6
4,574,858	+				-1.1
4,574,858	+				+0.5
4,574,858	+				-0.8
4,574,859	-				+0.0
4,574,859	-				-1.9
4,574,859	-				-2.1
4,574,859	-				+0.3
4,574,859	-				+0.9
4,574,859	-				+0.1
4,574,859	-				-0.7
4,574,859	-				-0.2
4,574,859	-				-0.3
4,574,859	-				-0.7
4,574,861	+				-0.4
4,574,861	+				-0.9
4,574,861	+				-1.9
4,574,861	+				-0.1
4,574,862	-				+0.1
4,574,862	-				+2.3
4,574,862	-				-2.1
4,574,862	-				+0.7
4,574,888	+				-1.2
4,574,888	+				-0.1
4,574,888	+				-1.0
4,574,888	+				+1.4
4,574,888	+				-1.1
4,574,889	-				+2.5
4,574,889	-				-0.2
4,574,889	-				+1.5
4,574,889	-				+1.2
4,574,909	-				-0.7
4,574,921	+				+0.9
4,574,921	+				+1.6
4,574,922	-				+0.7
4,574,922	-				+0.7
4,574,922	-				+1.2
4,574,922	-				+1.7
4,574,948	+				+0.8
4,574,948	+				+1.4
4,574,948	+				+0.9
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4,574,949	-				-0.1
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4,574,949	-				-0.2
4,574,949	-				-0.1
4,574,949	-				-0.2
4,574,972	+				+0.0
4,574,999	+				-1.1
4,574,999	+				-0.1
4,574,999	+				-0.4
4,575,000	-				-0.1
4,575,000	-				-0.9
4,575,000	-				+0.7
4,575,037	+				-0.9
4,575,037	+				-0.9
4,575,038	-				-0.2
4,575,041	+				-0.3
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4,575,042	-				-0.1
4,575,042	-				+0.6
4,575,042	-				+0.9
4,575,086	+				+1.7
4,575,086	+				-0.1
4,575,086	+				-0.1
4,575,087	-				+0.5
4,575,108	+				-0.7
4,575,119	+				+0.9
4,575,120	-				-2.8