Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08820 and MMP_RS08825 are separated by 171 nucleotides MMP_RS08825 and MMP_RS08830 are separated by 14 nucleotides MMP_RS08830 and MMP_RS08835 are separated by 10 nucleotides
MMP_RS08820: MMP_RS08820 - hypothetical protein, at 1,650,224 to 1,651,168
_RS08820
MMP_RS08825: MMP_RS08825 - roadblock/LC7 domain-containing protein, at 1,651,340 to 1,651,684
_RS08825
MMP_RS08830: MMP_RS08830 - GTP-binding protein, at 1,651,699 to 1,652,172
_RS08830
MMP_RS08835: MMP_RS08835 - H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein, at 1,652,183 to 1,653,208
_RS08835
Position (kb)
1651
1652
1653 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1650.737 kb on - strand, within MMP_RS08820 at 1650.819 kb on - strand, within MMP_RS08820 at 1650.894 kb on - strand, within MMP_RS08820 at 1650.894 kb on - strand, within MMP_RS08820 at 1651.194 kb on + strand at 1651.261 kb on - strand at 1651.261 kb on - strand at 1651.261 kb on - strand at 1651.262 kb on + strand at 1651.305 kb on - strand at 1651.305 kb on - strand at 1651.337 kb on + strand at 1651.684 kb on - strand at 1651.689 kb on + strand at 1651.700 kb on - strand at 1651.700 kb on - strand at 1651.797 kb on - strand, within MMP_RS08830 at 1651.797 kb on - strand, within MMP_RS08830 at 1651.798 kb on + strand, within MMP_RS08830 at 1651.802 kb on + strand, within MMP_RS08830 at 1651.946 kb on - strand, within MMP_RS08830 at 1652.043 kb on - strand, within MMP_RS08830 at 1652.054 kb on + strand, within MMP_RS08830 at 1652.157 kb on - strand at 1652.486 kb on - strand, within MMP_RS08835 at 1652.564 kb on - strand, within MMP_RS08835 at 1652.739 kb on - strand, within MMP_RS08835 at 1652.740 kb on + strand, within MMP_RS08835 at 1652.838 kb on - strand, within MMP_RS08835 at 1652.839 kb on + strand, within MMP_RS08835 at 1652.845 kb on - strand, within MMP_RS08835 at 1652.906 kb on + strand, within MMP_RS08835 at 1653.001 kb on - strand, within MMP_RS08835 at 1653.041 kb on - strand, within MMP_RS08835 at 1653.071 kb on + strand, within MMP_RS08835
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,650,737 - MMP_RS08820 0.54 -4.0 1,650,819 - MMP_RS08820 0.63 -4.3 1,650,894 - MMP_RS08820 0.71 -2.7 1,650,894 - MMP_RS08820 0.71 -2.1 1,651,194 + +0.3 1,651,261 - +0.4 1,651,261 - +0.4 1,651,261 - -0.0 1,651,262 + +0.3 1,651,305 - +0.5 1,651,305 - -0.1 1,651,337 + +0.8 1,651,684 - +0.8 1,651,689 + -0.1 1,651,700 - +0.0 1,651,700 - +0.5 1,651,797 - MMP_RS08830 0.21 +0.3 1,651,797 - MMP_RS08830 0.21 -0.1 1,651,798 + MMP_RS08830 0.21 +2.6 1,651,802 + MMP_RS08830 0.22 -0.4 1,651,946 - MMP_RS08830 0.52 +0.5 1,652,043 - MMP_RS08830 0.73 -0.3 1,652,054 + MMP_RS08830 0.75 +0.0 1,652,157 - +0.7 1,652,486 - MMP_RS08835 0.30 +0.4 1,652,564 - MMP_RS08835 0.37 +0.5 1,652,739 - MMP_RS08835 0.54 +0.0 1,652,740 + MMP_RS08835 0.54 +0.6 1,652,838 - MMP_RS08835 0.64 -0.0 1,652,839 + MMP_RS08835 0.64 +0.2 1,652,845 - MMP_RS08835 0.65 +0.6 1,652,906 + MMP_RS08835 0.70 +0.8 1,653,001 - MMP_RS08835 0.80 +4.2 1,653,041 - MMP_RS08835 0.84 -0.2 1,653,071 + MMP_RS08835 0.87 +0.1
Or see this region's nucleotide sequence