Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08745 and MMP_RS08750 are separated by 329 nucleotides MMP_RS08750 and MMP_RS08755 are separated by 0 nucleotides MMP_RS08755 and MMP_RS08760 are separated by 170 nucleotides MMP_RS08760 and MMP_RS08765 are separated by 11 nucleotides
MMP_RS08745: MMP_RS08745 - CDP-2 3-bis-(O-geranylgeranyl)-sn-glycerol synthase, at 1,639,662 to 1,640,198
_RS08745
MMP_RS08750: MMP_RS08750 - hypothetical protein, at 1,640,528 to 1,640,719
_RS08750
MMP_RS08755: MMP_RS08755 - sodium:solute symporter family protein, at 1,640,720 to 1,642,321
_RS08755
MMP_RS08760: MMP_RS08760 - hypothetical protein, at 1,642,492 to 1,642,974
_RS08760
MMP_RS08765: MMP_RS08765 - homoserine dehydrogenase, at 1,642,986 to 1,643,999
_RS08765
Position (kb)
1640
1641
1642
1643 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1639.736 kb on - strand, within MMP_RS08745 at 1639.810 kb on - strand, within MMP_RS08745 at 1639.874 kb on - strand, within MMP_RS08745 at 1639.880 kb on - strand, within MMP_RS08745 at 1639.881 kb on + strand, within MMP_RS08745 at 1639.882 kb on - strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.885 kb on - strand, within MMP_RS08745 at 1639.886 kb on + strand, within MMP_RS08745 at 1639.886 kb on + strand, within MMP_RS08745 at 1639.887 kb on - strand, within MMP_RS08745 at 1639.889 kb on - strand, within MMP_RS08745 at 1639.889 kb on - strand, within MMP_RS08745 at 1639.931 kb on - strand, within MMP_RS08745 at 1640.109 kb on + strand, within MMP_RS08745 at 1640.109 kb on + strand, within MMP_RS08745 at 1640.120 kb on + strand, within MMP_RS08745 at 1640.196 kb on - strand at 1640.244 kb on + strand at 1640.244 kb on + strand at 1640.245 kb on - strand at 1640.245 kb on - strand at 1640.249 kb on - strand at 1640.250 kb on + strand at 1640.250 kb on + strand at 1640.250 kb on + strand at 1640.250 kb on + strand at 1640.252 kb on - strand at 1640.262 kb on - strand at 1640.412 kb on + strand at 1640.450 kb on - strand at 1640.450 kb on - strand at 1640.451 kb on + strand at 1640.451 kb on + strand at 1640.451 kb on + strand at 1640.455 kb on - strand at 1640.466 kb on - strand at 1640.479 kb on + strand at 1640.479 kb on + strand at 1640.601 kb on - strand, within MMP_RS08750 at 1640.601 kb on - strand, within MMP_RS08750 at 1640.602 kb on + strand, within MMP_RS08750 at 1640.736 kb on - strand at 1640.752 kb on + strand at 1640.755 kb on + strand at 1640.756 kb on - strand at 1640.756 kb on - strand at 1640.757 kb on + strand at 1640.763 kb on - strand at 1640.925 kb on - strand, within MMP_RS08755 at 1640.975 kb on + strand, within MMP_RS08755 at 1640.981 kb on + strand, within MMP_RS08755 at 1640.982 kb on - strand, within MMP_RS08755 at 1641.010 kb on - strand, within MMP_RS08755 at 1641.082 kb on - strand, within MMP_RS08755 at 1641.084 kb on - strand, within MMP_RS08755 at 1641.106 kb on + strand, within MMP_RS08755 at 1641.166 kb on + strand, within MMP_RS08755 at 1641.289 kb on - strand, within MMP_RS08755 at 1641.290 kb on + strand, within MMP_RS08755 at 1641.316 kb on + strand, within MMP_RS08755 at 1641.317 kb on - strand, within MMP_RS08755 at 1641.317 kb on - strand, within MMP_RS08755 at 1641.318 kb on + strand, within MMP_RS08755 at 1641.326 kb on - strand, within MMP_RS08755 at 1641.326 kb on - strand, within MMP_RS08755 at 1641.327 kb on + strand, within MMP_RS08755 at 1641.374 kb on + strand, within MMP_RS08755 at 1641.374 kb on + strand, within MMP_RS08755 at 1641.374 kb on + strand, within MMP_RS08755 at 1641.615 kb on + strand, within MMP_RS08755 at 1641.652 kb on - strand, within MMP_RS08755 at 1641.838 kb on - strand, within MMP_RS08755 at 1641.855 kb on + strand, within MMP_RS08755 at 1641.891 kb on - strand, within MMP_RS08755 at 1641.895 kb on - strand, within MMP_RS08755 at 1641.896 kb on + strand, within MMP_RS08755 at 1642.091 kb on + strand, within MMP_RS08755 at 1642.260 kb on - strand at 1642.423 kb on + strand at 1642.438 kb on + strand at 1642.452 kb on - strand at 1642.453 kb on + strand at 1642.453 kb on + strand at 1642.455 kb on - strand at 1642.459 kb on - strand at 1642.460 kb on + strand at 1642.460 kb on + strand at 1642.465 kb on + strand at 1642.632 kb on + strand, within MMP_RS08760 at 1642.675 kb on + strand, within MMP_RS08760 at 1642.890 kb on + strand, within MMP_RS08760 at 1643.153 kb on + strand at 1643.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,639,736 - MMP_RS08745 0.14 -2.4 1,639,810 - MMP_RS08745 0.28 -2.0 1,639,874 - MMP_RS08745 0.39 -3.4 1,639,880 - MMP_RS08745 0.41 -1.9 1,639,881 + MMP_RS08745 0.41 -2.3 1,639,882 - MMP_RS08745 0.41 -1.0 1,639,883 + MMP_RS08745 0.41 -4.9 1,639,883 + MMP_RS08745 0.41 -0.9 1,639,883 + MMP_RS08745 0.41 -4.3 1,639,885 - MMP_RS08745 0.42 -1.1 1,639,886 + MMP_RS08745 0.42 -3.1 1,639,886 + MMP_RS08745 0.42 -4.4 1,639,887 - MMP_RS08745 0.42 -2.3 1,639,889 - MMP_RS08745 0.42 -2.5 1,639,889 - MMP_RS08745 0.42 -3.3 1,639,931 - MMP_RS08745 0.50 -3.7 1,640,109 + MMP_RS08745 0.83 -4.3 1,640,109 + MMP_RS08745 0.83 -2.0 1,640,120 + MMP_RS08745 0.85 -4.1 1,640,196 - +0.3 1,640,244 + +0.3 1,640,244 + +0.1 1,640,245 - +0.1 1,640,245 - +0.5 1,640,249 - +0.5 1,640,250 + +0.2 1,640,250 + +0.3 1,640,250 + +0.1 1,640,250 + +0.4 1,640,252 - -0.2 1,640,262 - +0.2 1,640,412 + +0.2 1,640,450 - +0.2 1,640,450 - +0.7 1,640,451 + +0.5 1,640,451 + +0.4 1,640,451 + +0.5 1,640,455 - +0.6 1,640,466 - -0.1 1,640,479 + +0.6 1,640,479 + +0.4 1,640,601 - MMP_RS08750 0.38 +0.8 1,640,601 - MMP_RS08750 0.38 +0.7 1,640,602 + MMP_RS08750 0.39 -0.0 1,640,736 - +0.0 1,640,752 + +0.5 1,640,755 + +0.4 1,640,756 - +0.2 1,640,756 - -0.2 1,640,757 + +0.3 1,640,763 - +0.8 1,640,925 - MMP_RS08755 0.13 -0.0 1,640,975 + MMP_RS08755 0.16 +0.3 1,640,981 + MMP_RS08755 0.16 +0.4 1,640,982 - MMP_RS08755 0.16 -0.3 1,641,010 - MMP_RS08755 0.18 +0.6 1,641,082 - MMP_RS08755 0.23 +0.1 1,641,084 - MMP_RS08755 0.23 +0.8 1,641,106 + MMP_RS08755 0.24 +0.3 1,641,166 + MMP_RS08755 0.28 +0.1 1,641,289 - MMP_RS08755 0.36 +0.3 1,641,290 + MMP_RS08755 0.36 +0.4 1,641,316 + MMP_RS08755 0.37 +0.5 1,641,317 - MMP_RS08755 0.37 +0.6 1,641,317 - MMP_RS08755 0.37 +0.4 1,641,318 + MMP_RS08755 0.37 -0.0 1,641,326 - MMP_RS08755 0.38 -0.2 1,641,326 - MMP_RS08755 0.38 +0.3 1,641,327 + MMP_RS08755 0.38 +0.9 1,641,374 + MMP_RS08755 0.41 +0.2 1,641,374 + MMP_RS08755 0.41 -0.0 1,641,374 + MMP_RS08755 0.41 +0.6 1,641,615 + MMP_RS08755 0.56 +0.1 1,641,652 - MMP_RS08755 0.58 +0.6 1,641,838 - MMP_RS08755 0.70 +0.3 1,641,855 + MMP_RS08755 0.71 +0.3 1,641,891 - MMP_RS08755 0.73 +0.0 1,641,895 - MMP_RS08755 0.73 +0.4 1,641,896 + MMP_RS08755 0.73 +0.5 1,642,091 + MMP_RS08755 0.86 +1.0 1,642,260 - -0.4 1,642,423 + +0.7 1,642,438 + -0.5 1,642,452 - -4.9 1,642,453 + -4.1 1,642,453 + -6.4 1,642,455 - -5.4 1,642,459 - -3.0 1,642,460 + -0.5 1,642,460 + +0.5 1,642,465 + -0.0 1,642,632 + MMP_RS08760 0.29 -3.5 1,642,675 + MMP_RS08760 0.38 -0.8 1,642,890 + MMP_RS08760 0.82 +0.1 1,643,153 + -3.9 1,643,315 + -3.5
Or see this region's nucleotide sequence