Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08370

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08365 and MMP_RS08370 are separated by 25 nucleotidesMMP_RS08370 and MMP_RS08375 are separated by 152 nucleotides MMP_RS08365: MMP_RS08365 - energy conserving hydrogenase EhbF, at 1,570,763 to 1,572,211 _RS08365 MMP_RS08370: MMP_RS08370 - cation:proton antiporter subunit C, at 1,572,237 to 1,572,593 _RS08370 MMP_RS08375: MMP_RS08375 - ATP-binding cassette domain-containing protein, at 1,572,746 to 1,574,395 _RS08375 Position (kb) 1572 1573Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1571.549 kb on - strand, within MMP_RS08365at 1571.618 kb on - strand, within MMP_RS08365at 1571.618 kb on - strand, within MMP_RS08365at 1571.618 kb on - strand, within MMP_RS08365at 1571.882 kb on - strand, within MMP_RS08365at 1572.004 kb on + strand, within MMP_RS08365at 1572.111 kb on + strandat 1572.292 kb on + strand, within MMP_RS08370at 1572.667 kb on - strandat 1572.697 kb on - strandat 1572.697 kb on - strandat 1572.698 kb on + strandat 1572.699 kb on - strandat 1572.700 kb on + strandat 1572.700 kb on + strandat 1572.700 kb on + strandat 1572.701 kb on - strandat 1572.714 kb on + strandat 1573.215 kb on + strandat 1573.472 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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1,571,549 - MMP_RS08365 0.54 +1.2
1,571,618 - MMP_RS08365 0.59 +1.8
1,571,618 - MMP_RS08365 0.59 +2.4
1,571,618 - MMP_RS08365 0.59 +0.8
1,571,882 - MMP_RS08365 0.77 +0.9
1,572,004 + MMP_RS08365 0.86 +1.1
1,572,111 + +1.2
1,572,292 + MMP_RS08370 0.15 +1.2
1,572,667 - +1.2
1,572,697 - +1.7
1,572,697 - +1.2
1,572,698 + +1.0
1,572,699 - -0.4
1,572,700 + +1.6
1,572,700 + +1.5
1,572,700 + +1.0
1,572,701 - -1.1
1,572,714 + +1.1
1,573,215 + -2.4
1,573,472 - -2.8

Or see this region's nucleotide sequence