Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08240 and MMP_RS08245 are separated by 24 nucleotides MMP_RS08245 and MMP_RS08250 are separated by 109 nucleotides
MMP_RS08240: MMP_RS08240 - methanogenesis marker 16 metalloprotein, at 1,550,226 to 1,551,392
_RS08240
MMP_RS08245: MMP_RS08245 - DUF4013 domain-containing protein, at 1,551,417 to 1,552,286
_RS08245
MMP_RS08250: MMP_RS08250 - pyruvate kinase, at 1,552,396 to 1,553,739
_RS08250
Position (kb)
1551
1552
1553 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1550.496 kb on - strand, within MMP_RS08240 at 1550.678 kb on + strand, within MMP_RS08240 at 1550.743 kb on - strand, within MMP_RS08240 at 1550.901 kb on - strand, within MMP_RS08240 at 1551.105 kb on + strand, within MMP_RS08240 at 1551.265 kb on - strand, within MMP_RS08240 at 1551.266 kb on + strand, within MMP_RS08240 at 1551.268 kb on + strand, within MMP_RS08240 at 1551.338 kb on + strand at 1551.357 kb on + strand at 1551.415 kb on + strand at 1551.609 kb on - strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.682 kb on + strand, within MMP_RS08245 at 1551.769 kb on - strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.864 kb on - strand, within MMP_RS08245 at 1551.865 kb on + strand, within MMP_RS08245 at 1551.894 kb on - strand, within MMP_RS08245 at 1552.036 kb on - strand, within MMP_RS08245 at 1552.107 kb on + strand, within MMP_RS08245 at 1552.271 kb on + strand at 1552.271 kb on + strand at 1552.314 kb on + strand at 1552.328 kb on + strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.330 kb on + strand at 1552.330 kb on + strand at 1552.333 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.365 kb on - strand at 1552.365 kb on - strand at 1552.369 kb on - strand at 1552.380 kb on + strand at 1552.444 kb on + strand at 1552.561 kb on + strand, within MMP_RS08250 at 1552.681 kb on - strand, within MMP_RS08250 at 1552.770 kb on + strand, within MMP_RS08250 at 1552.799 kb on - strand, within MMP_RS08250 at 1552.863 kb on - strand, within MMP_RS08250 at 1552.866 kb on + strand, within MMP_RS08250 at 1552.970 kb on + strand, within MMP_RS08250 at 1553.125 kb on + strand, within MMP_RS08250 at 1553.159 kb on - strand, within MMP_RS08250 at 1553.164 kb on + strand, within MMP_RS08250 at 1553.193 kb on + strand, within MMP_RS08250
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,550,496 - MMP_RS08240 0.23 -1.1 1,550,678 + MMP_RS08240 0.39 -1.2 1,550,743 - MMP_RS08240 0.44 -1.2 1,550,901 - MMP_RS08240 0.58 -1.3 1,551,105 + MMP_RS08240 0.75 -0.5 1,551,265 - MMP_RS08240 0.89 -1.7 1,551,266 + MMP_RS08240 0.89 -1.0 1,551,268 + MMP_RS08240 0.89 -0.4 1,551,338 + -1.1 1,551,357 + -0.7 1,551,415 + +0.6 1,551,609 - MMP_RS08245 0.22 +3.4 1,551,610 + MMP_RS08245 0.22 -0.0 1,551,610 + MMP_RS08245 0.22 +1.6 1,551,682 + MMP_RS08245 0.30 -0.2 1,551,769 - MMP_RS08245 0.40 +0.3 1,551,770 + MMP_RS08245 0.41 +0.0 1,551,770 + MMP_RS08245 0.41 -0.6 1,551,864 - MMP_RS08245 0.51 +0.5 1,551,865 + MMP_RS08245 0.51 +0.7 1,551,894 - MMP_RS08245 0.55 -0.2 1,552,036 - MMP_RS08245 0.71 -0.2 1,552,107 + MMP_RS08245 0.79 -0.4 1,552,271 + +0.2 1,552,271 + +0.4 1,552,314 + -0.1 1,552,328 + -0.1 1,552,329 - -0.0 1,552,329 - +0.2 1,552,329 - -0.5 1,552,329 - +0.4 1,552,330 + -0.5 1,552,330 + +0.2 1,552,333 - +0.5 1,552,361 - +0.1 1,552,361 - +0.6 1,552,361 - +0.2 1,552,362 + +0.4 1,552,362 + +0.1 1,552,362 + -0.4 1,552,365 - +0.0 1,552,365 - +0.6 1,552,369 - -0.3 1,552,380 + +0.5 1,552,444 + -0.1 1,552,561 + MMP_RS08250 0.12 +0.6 1,552,681 - MMP_RS08250 0.21 +0.5 1,552,770 + MMP_RS08250 0.28 -0.2 1,552,799 - MMP_RS08250 0.30 +0.9 1,552,863 - MMP_RS08250 0.35 +0.3 1,552,866 + MMP_RS08250 0.35 +0.3 1,552,970 + MMP_RS08250 0.43 +3.1 1,553,125 + MMP_RS08250 0.54 +0.1 1,553,159 - MMP_RS08250 0.57 -0.0 1,553,164 + MMP_RS08250 0.57 +0.2 1,553,193 + MMP_RS08250 0.59 +0.0
Or see this region's nucleotide sequence