Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08075 and MMP_RS08080 are separated by 10 nucleotides MMP_RS08080 and MMP_RS08085 are separated by 8 nucleotides MMP_RS08085 and MMP_RS08090 are separated by 88 nucleotides MMP_RS08090 and MMP_RS08095 are separated by 11 nucleotides
MMP_RS08075: MMP_RS08075 - DUF749 domain-containing protein, at 1,521,019 to 1,521,327
_RS08075
MMP_RS08080: MMP_RS08080 - YchF/TatD family DNA exonuclease, at 1,521,338 to 1,522,093
_RS08080
MMP_RS08085: MMP_RS08085 - hypothetical protein, at 1,522,102 to 1,523,037
_RS08085
MMP_RS08090: MMP_RS08090 - hypothetical protein, at 1,523,126 to 1,523,422
_RS08090
MMP_RS08095: MMP_RS08095 - dethiobiotin synthase, at 1,523,434 to 1,524,006
_RS08095
Position (kb)
1522
1523
1524 Strain fitness (log2 ratio)
-1
0
1 at 1521.151 kb on - strand, within MMP_RS08075 at 1521.152 kb on + strand, within MMP_RS08075 at 1521.158 kb on - strand, within MMP_RS08075 at 1521.186 kb on - strand, within MMP_RS08075 at 1521.190 kb on + strand, within MMP_RS08075 at 1521.348 kb on - strand at 1521.350 kb on - strand at 1521.369 kb on - strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.373 kb on - strand at 1521.493 kb on - strand, within MMP_RS08080 at 1521.493 kb on - strand, within MMP_RS08080 at 1521.564 kb on + strand, within MMP_RS08080 at 1521.572 kb on + strand, within MMP_RS08080 at 1521.577 kb on + strand, within MMP_RS08080 at 1521.755 kb on - strand, within MMP_RS08080 at 1521.849 kb on - strand, within MMP_RS08080 at 1521.868 kb on - strand, within MMP_RS08080 at 1521.881 kb on + strand, within MMP_RS08080 at 1521.909 kb on + strand, within MMP_RS08080 at 1521.977 kb on - strand, within MMP_RS08080 at 1522.010 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.071 kb on - strand at 1522.076 kb on + strand at 1522.086 kb on + strand at 1522.123 kb on - strand at 1522.265 kb on + strand, within MMP_RS08085 at 1522.309 kb on - strand, within MMP_RS08085 at 1522.349 kb on + strand, within MMP_RS08085 at 1522.377 kb on - strand, within MMP_RS08085 at 1522.387 kb on - strand, within MMP_RS08085 at 1522.414 kb on - strand, within MMP_RS08085 at 1522.458 kb on + strand, within MMP_RS08085 at 1522.479 kb on - strand, within MMP_RS08085 at 1522.480 kb on + strand, within MMP_RS08085 at 1522.570 kb on + strand, within MMP_RS08085 at 1522.595 kb on + strand, within MMP_RS08085 at 1522.658 kb on - strand, within MMP_RS08085 at 1522.664 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.707 kb on + strand, within MMP_RS08085 at 1522.727 kb on + strand, within MMP_RS08085 at 1522.847 kb on + strand, within MMP_RS08085 at 1522.859 kb on + strand, within MMP_RS08085 at 1522.951 kb on + strand at 1522.952 kb on - strand at 1522.991 kb on - strand at 1523.023 kb on - strand at 1523.024 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.040 kb on - strand at 1523.064 kb on - strand at 1523.088 kb on + strand at 1523.091 kb on - strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.093 kb on - strand at 1523.093 kb on - strand at 1523.094 kb on + strand at 1523.095 kb on - strand at 1523.095 kb on - strand at 1523.104 kb on + strand at 1523.104 kb on + strand at 1523.156 kb on - strand, within MMP_RS08090 at 1523.367 kb on - strand, within MMP_RS08090 at 1523.371 kb on + strand, within MMP_RS08090 at 1523.371 kb on + strand, within MMP_RS08090 at 1523.392 kb on + strand, within MMP_RS08090 at 1523.451 kb on - strand at 1523.575 kb on + strand, within MMP_RS08095 at 1523.707 kb on - strand, within MMP_RS08095 at 1523.758 kb on - strand, within MMP_RS08095 at 1523.762 kb on - strand, within MMP_RS08095 at 1523.763 kb on + strand, within MMP_RS08095 at 1523.801 kb on + strand, within MMP_RS08095 at 1523.815 kb on - strand, within MMP_RS08095 at 1523.920 kb on + strand, within MMP_RS08095 at 1523.924 kb on - strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.931 kb on - strand, within MMP_RS08095 at 1523.962 kb on - strand at 1523.965 kb on + strand at 1523.965 kb on + strand at 1523.965 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,521,151 - MMP_RS08075 0.43 -0.1 1,521,152 + MMP_RS08075 0.43 -0.0 1,521,158 - MMP_RS08075 0.45 +0.0 1,521,186 - MMP_RS08075 0.54 -0.5 1,521,190 + MMP_RS08075 0.55 +0.3 1,521,348 - -0.0 1,521,350 - -0.0 1,521,369 - -0.3 1,521,370 + -0.2 1,521,370 + +0.1 1,521,370 + -0.9 1,521,373 - +0.1 1,521,493 - MMP_RS08080 0.21 -0.2 1,521,493 - MMP_RS08080 0.21 +0.0 1,521,564 + MMP_RS08080 0.30 +0.1 1,521,572 + MMP_RS08080 0.31 -0.4 1,521,577 + MMP_RS08080 0.32 +0.3 1,521,755 - MMP_RS08080 0.55 -0.2 1,521,849 - MMP_RS08080 0.68 -0.0 1,521,868 - MMP_RS08080 0.70 +0.2 1,521,881 + MMP_RS08080 0.72 -0.7 1,521,909 + MMP_RS08080 0.76 +0.4 1,521,977 - MMP_RS08080 0.85 +0.2 1,522,010 - MMP_RS08080 0.89 -0.2 1,522,015 - MMP_RS08080 0.90 +0.0 1,522,015 - MMP_RS08080 0.90 +0.0 1,522,071 - +0.2 1,522,076 + +0.3 1,522,086 + -0.2 1,522,123 - +0.4 1,522,265 + MMP_RS08085 0.17 +0.5 1,522,309 - MMP_RS08085 0.22 +0.1 1,522,349 + MMP_RS08085 0.26 +0.6 1,522,377 - MMP_RS08085 0.29 -0.5 1,522,387 - MMP_RS08085 0.30 +0.9 1,522,414 - MMP_RS08085 0.33 +1.0 1,522,458 + MMP_RS08085 0.38 +0.3 1,522,479 - MMP_RS08085 0.40 +0.8 1,522,480 + MMP_RS08085 0.40 +0.4 1,522,570 + MMP_RS08085 0.50 -0.4 1,522,595 + MMP_RS08085 0.53 +0.4 1,522,658 - MMP_RS08085 0.59 +1.1 1,522,664 + MMP_RS08085 0.60 +0.7 1,522,672 + MMP_RS08085 0.61 +0.6 1,522,672 + MMP_RS08085 0.61 +0.9 1,522,704 + MMP_RS08085 0.64 +0.3 1,522,704 + MMP_RS08085 0.64 +0.3 1,522,707 + MMP_RS08085 0.65 +0.3 1,522,727 + MMP_RS08085 0.67 +0.7 1,522,847 + MMP_RS08085 0.80 +0.7 1,522,859 + MMP_RS08085 0.81 +0.1 1,522,951 + +0.6 1,522,952 - +0.6 1,522,991 - -0.5 1,523,023 - +0.6 1,523,024 + +0.0 1,523,029 + +0.4 1,523,029 + +0.4 1,523,029 + -0.2 1,523,029 + -0.2 1,523,040 - +0.2 1,523,064 - +0.2 1,523,088 + +0.5 1,523,091 - +0.2 1,523,092 + +0.3 1,523,092 + -0.1 1,523,092 + -1.1 1,523,093 - -0.0 1,523,093 - +0.0 1,523,094 + +0.3 1,523,095 - +0.8 1,523,095 - +0.0 1,523,104 + +1.0 1,523,104 + +0.5 1,523,156 - MMP_RS08090 0.10 +0.5 1,523,367 - MMP_RS08090 0.81 -0.3 1,523,371 + MMP_RS08090 0.82 +0.8 1,523,371 + MMP_RS08090 0.82 +0.1 1,523,392 + MMP_RS08090 0.90 -0.0 1,523,451 - +0.3 1,523,575 + MMP_RS08095 0.25 +0.2 1,523,707 - MMP_RS08095 0.48 -0.0 1,523,758 - MMP_RS08095 0.57 -0.5 1,523,762 - MMP_RS08095 0.57 -0.6 1,523,763 + MMP_RS08095 0.57 +0.6 1,523,801 + MMP_RS08095 0.64 +0.0 1,523,815 - MMP_RS08095 0.66 +0.1 1,523,920 + MMP_RS08095 0.85 +0.3 1,523,924 - MMP_RS08095 0.86 -0.1 1,523,925 + MMP_RS08095 0.86 -0.9 1,523,925 + MMP_RS08095 0.86 -0.3 1,523,925 + MMP_RS08095 0.86 -0.3 1,523,931 - MMP_RS08095 0.87 -0.4 1,523,962 - -0.9 1,523,965 + -0.5 1,523,965 + +0.2 1,523,965 + +0.6
Or see this region's nucleotide sequence