Strain Fitness in Methanococcus maripaludis S2 around MMP_RS07835

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS07825 and MMP_RS07830 are separated by 17 nucleotidesMMP_RS07830 and MMP_RS07835 are separated by 107 nucleotidesMMP_RS07835 and MMP_RS07840 are separated by 51 nucleotidesMMP_RS07840 and MMP_RS07845 are separated by 42 nucleotides MMP_RS07825: MMP_RS07825 - preprotein translocase subunit SecD, at 1,481,881 to 1,483,047 _RS07825 MMP_RS07830: MMP_RS07830 - protein translocase subunit SecF, at 1,483,065 to 1,483,910 _RS07830 MMP_RS07835: MMP_RS07835 - TldD/PmbA family protein, at 1,484,018 to 1,485,280 _RS07835 MMP_RS07840: MMP_RS07840 - ribonuclease III, at 1,485,332 to 1,486,021 _RS07840 MMP_RS07845: MMP_RS07845 - LL-diaminopimelate aminotransferase, at 1,486,064 to 1,487,314 _RS07845 Position (kb) 1484 1485 1486Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1483.093 kb on - strandat 1483.792 kb on + strandat 1483.963 kb on + strandat 1483.966 kb on - strandat 1483.982 kb on + strandat 1483.994 kb on - strandat 1484.241 kb on - strand, within MMP_RS07835at 1484.876 kb on + strand, within MMP_RS07835at 1484.998 kb on - strand, within MMP_RS07835at 1485.042 kb on + strand, within MMP_RS07835at 1485.047 kb on - strand, within MMP_RS07835at 1485.123 kb on + strand, within MMP_RS07835at 1485.313 kb on - strandat 1485.370 kb on + strandat 1485.385 kb on + strandat 1485.395 kb on + strandat 1485.566 kb on - strand, within MMP_RS07840at 1485.761 kb on + strand, within MMP_RS07840at 1486.083 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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1,483,093 - -1.1
1,483,792 + -2.6
1,483,963 + -3.0
1,483,966 - -4.4
1,483,982 + -1.2
1,483,994 - -1.9
1,484,241 - MMP_RS07835 0.18 +0.1
1,484,876 + MMP_RS07835 0.68 +0.9
1,484,998 - MMP_RS07835 0.78 +0.4
1,485,042 + MMP_RS07835 0.81 +1.0
1,485,047 - MMP_RS07835 0.81 -0.1
1,485,123 + MMP_RS07835 0.87 +0.2
1,485,313 - +1.2
1,485,370 + +0.3
1,485,385 + -0.0
1,485,395 + -0.3
1,485,566 - MMP_RS07840 0.34 +0.1
1,485,761 + MMP_RS07840 0.62 +0.3
1,486,083 + -4.9

Or see this region's nucleotide sequence