Strain Fitness in Methanococcus maripaludis S2 around MMP_RS07125

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS07120 and MMP_RS07125 are separated by 102 nucleotidesMMP_RS07125 and MMP_RS07130 are separated by 14 nucleotidesMMP_RS07130 and MMP_RS07135 are separated by 33 nucleotides MMP_RS07120: MMP_RS07120 - coenzyme F420 hydrogenase subunit alpha, at 1,362,470 to 1,363,714 _RS07120 MMP_RS07125: MMP_RS07125 - coenzyme F420-reducing hydrogenase FrhD protein, at 1,363,817 to 1,364,341 _RS07125 MMP_RS07130: MMP_RS07130 - coenzyme F420 hydrogenase subunit gamma, at 1,364,356 to 1,365,042 _RS07130 MMP_RS07135: MMP_RS07135 - coenzyme F420 hydrogenase subunit beta, at 1,365,076 to 1,365,924 _RS07135 Position (kb) 1363 1364 1365Strain fitness (log2 ratio) -1 0 1 2 3 4at 1362.853 kb on + strand, within MMP_RS07120at 1362.904 kb on + strand, within MMP_RS07120at 1363.039 kb on + strand, within MMP_RS07120at 1363.111 kb on + strand, within MMP_RS07120at 1363.111 kb on + strand, within MMP_RS07120at 1363.115 kb on - strand, within MMP_RS07120at 1363.116 kb on + strand, within MMP_RS07120at 1363.193 kb on - strand, within MMP_RS07120at 1363.247 kb on - strand, within MMP_RS07120at 1363.248 kb on + strand, within MMP_RS07120at 1363.399 kb on + strand, within MMP_RS07120at 1363.535 kb on - strand, within MMP_RS07120at 1363.617 kb on - strandat 1363.638 kb on - strandat 1363.638 kb on - strandat 1363.824 kb on + strandat 1363.899 kb on - strand, within MMP_RS07125at 1363.971 kb on - strand, within MMP_RS07125at 1364.098 kb on - strand, within MMP_RS07125at 1364.176 kb on + strand, within MMP_RS07125at 1364.308 kb on + strandat 1364.381 kb on - strandat 1364.508 kb on + strand, within MMP_RS07130at 1364.513 kb on - strand, within MMP_RS07130at 1364.594 kb on - strand, within MMP_RS07130at 1364.598 kb on + strand, within MMP_RS07130at 1364.631 kb on - strand, within MMP_RS07130at 1364.753 kb on - strand, within MMP_RS07130at 1364.754 kb on + strand, within MMP_RS07130at 1364.798 kb on - strand, within MMP_RS07130at 1364.798 kb on - strand, within MMP_RS07130at 1364.807 kb on + strand, within MMP_RS07130at 1364.928 kb on + strand, within MMP_RS07130at 1364.928 kb on + strand, within MMP_RS07130at 1364.932 kb on + strand, within MMP_RS07130at 1365.035 kb on - strandat 1365.040 kb on - strandat 1365.040 kb on - strandat 1365.040 kb on - strandat 1365.098 kb on - strandat 1365.188 kb on - strand, within MMP_RS07135at 1365.262 kb on + strand, within MMP_RS07135at 1365.304 kb on + strand, within MMP_RS07135

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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1,362,853 + MMP_RS07120 0.31 +0.0
1,362,904 + MMP_RS07120 0.35 -0.4
1,363,039 + MMP_RS07120 0.46 +0.0
1,363,111 + MMP_RS07120 0.51 +0.5
1,363,111 + MMP_RS07120 0.51 +0.7
1,363,115 - MMP_RS07120 0.52 +0.6
1,363,116 + MMP_RS07120 0.52 -0.3
1,363,193 - MMP_RS07120 0.58 -0.2
1,363,247 - MMP_RS07120 0.62 +0.2
1,363,248 + MMP_RS07120 0.62 +0.1
1,363,399 + MMP_RS07120 0.75 +0.1
1,363,535 - MMP_RS07120 0.86 +0.8
1,363,617 - +0.1
1,363,638 - -0.2
1,363,638 - -0.5
1,363,824 + +1.1
1,363,899 - MMP_RS07125 0.16 +0.3
1,363,971 - MMP_RS07125 0.29 +0.1
1,364,098 - MMP_RS07125 0.54 +1.0
1,364,176 + MMP_RS07125 0.68 +0.5
1,364,308 + +1.0
1,364,381 - -0.1
1,364,508 + MMP_RS07130 0.22 +0.4
1,364,513 - MMP_RS07130 0.23 +1.1
1,364,594 - MMP_RS07130 0.35 -0.2
1,364,598 + MMP_RS07130 0.35 +0.2
1,364,631 - MMP_RS07130 0.40 +0.8
1,364,753 - MMP_RS07130 0.58 -0.4
1,364,754 + MMP_RS07130 0.58 +0.6
1,364,798 - MMP_RS07130 0.64 -0.5
1,364,798 - MMP_RS07130 0.64 +0.6
1,364,807 + MMP_RS07130 0.66 +0.5
1,364,928 + MMP_RS07130 0.83 +4.5
1,364,928 + MMP_RS07130 0.83 +0.4
1,364,932 + MMP_RS07130 0.84 +0.6
1,365,035 - +0.8
1,365,040 - -0.2
1,365,040 - +0.1
1,365,040 - +0.3
1,365,098 - -0.4
1,365,188 - MMP_RS07135 0.13 -0.3
1,365,262 + MMP_RS07135 0.22 +0.2
1,365,304 + MMP_RS07135 0.27 +0.8

Or see this region's nucleotide sequence