Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07085 and MMP_RS07090 overlap by 1 nucleotides MMP_RS07090 and MMP_RS07095 are separated by 89 nucleotides MMP_RS07095 and MMP_RS07100 are separated by 32 nucleotides
MMP_RS07085: MMP_RS07085 - ribonuclease HII, at 1,355,645 to 1,356,373
_RS07085
MMP_RS07090: MMP_RS07090 - tRNA (adenine-N1)-methyltransferase, at 1,356,373 to 1,357,251
_RS07090
MMP_RS07095: MMP_RS07095 - PspC domain-containing protein, at 1,357,341 to 1,357,583
_RS07095
MMP_RS07100: MMP_RS07100 - hypothetical protein, at 1,357,616 to 1,358,683
_RS07100
Position (kb)
1357
1358 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1356.379 kb on + strand at 1356.393 kb on - strand at 1356.395 kb on - strand at 1356.395 kb on - strand at 1356.396 kb on + strand at 1356.605 kb on - strand, within MMP_RS07090 at 1356.658 kb on + strand, within MMP_RS07090 at 1356.675 kb on - strand, within MMP_RS07090 at 1356.929 kb on - strand, within MMP_RS07090 at 1356.930 kb on + strand, within MMP_RS07090 at 1356.933 kb on - strand, within MMP_RS07090 at 1357.004 kb on - strand, within MMP_RS07090 at 1357.005 kb on + strand, within MMP_RS07090 at 1357.006 kb on - strand, within MMP_RS07090 at 1357.006 kb on - strand, within MMP_RS07090 at 1357.006 kb on - strand, within MMP_RS07090 at 1357.007 kb on + strand, within MMP_RS07090 at 1357.007 kb on + strand, within MMP_RS07090 at 1357.007 kb on + strand, within MMP_RS07090 at 1357.015 kb on - strand, within MMP_RS07090 at 1357.016 kb on + strand, within MMP_RS07090 at 1357.071 kb on - strand, within MMP_RS07090 at 1357.074 kb on - strand, within MMP_RS07090 at 1357.075 kb on + strand, within MMP_RS07090 at 1357.090 kb on + strand, within MMP_RS07090 at 1357.090 kb on + strand, within MMP_RS07090 at 1357.090 kb on + strand, within MMP_RS07090 at 1357.094 kb on - strand, within MMP_RS07090 at 1357.095 kb on + strand, within MMP_RS07090 at 1357.159 kb on - strand, within MMP_RS07090 at 1357.161 kb on - strand, within MMP_RS07090 at 1357.164 kb on - strand at 1357.165 kb on + strand at 1357.298 kb on - strand at 1357.302 kb on + strand at 1357.323 kb on - strand at 1357.341 kb on + strand at 1357.388 kb on + strand, within MMP_RS07095 at 1357.417 kb on - strand, within MMP_RS07095 at 1357.417 kb on - strand, within MMP_RS07095 at 1357.450 kb on - strand, within MMP_RS07095 at 1357.487 kb on - strand, within MMP_RS07095 at 1357.587 kb on + strand at 1357.665 kb on - strand at 1357.914 kb on + strand, within MMP_RS07100 at 1357.955 kb on - strand, within MMP_RS07100 at 1357.992 kb on + strand, within MMP_RS07100 at 1357.994 kb on + strand, within MMP_RS07100 at 1357.994 kb on + strand, within MMP_RS07100 at 1357.996 kb on - strand, within MMP_RS07100 at 1357.999 kb on - strand, within MMP_RS07100 at 1358.061 kb on - strand, within MMP_RS07100 at 1358.105 kb on - strand, within MMP_RS07100 at 1358.177 kb on - strand, within MMP_RS07100 at 1358.211 kb on - strand, within MMP_RS07100 at 1358.212 kb on + strand, within MMP_RS07100 at 1358.212 kb on + strand, within MMP_RS07100 at 1358.213 kb on - strand, within MMP_RS07100 at 1358.224 kb on + strand, within MMP_RS07100 at 1358.357 kb on - strand, within MMP_RS07100 at 1358.385 kb on + strand, within MMP_RS07100 at 1358.429 kb on - strand, within MMP_RS07100 at 1358.429 kb on - strand, within MMP_RS07100 at 1358.430 kb on + strand, within MMP_RS07100 at 1358.446 kb on + strand, within MMP_RS07100 at 1358.446 kb on + strand, within MMP_RS07100 at 1358.520 kb on + strand, within MMP_RS07100 at 1358.570 kb on + strand, within MMP_RS07100
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,356,379 + -4.4 1,356,393 - -3.9 1,356,395 - -2.7 1,356,395 - -4.3 1,356,396 + -3.4 1,356,605 - MMP_RS07090 0.26 -4.0 1,356,658 + MMP_RS07090 0.32 -3.0 1,356,675 - MMP_RS07090 0.34 -4.0 1,356,929 - MMP_RS07090 0.63 -3.2 1,356,930 + MMP_RS07090 0.63 -4.1 1,356,933 - MMP_RS07090 0.64 -2.3 1,357,004 - MMP_RS07090 0.72 -1.3 1,357,005 + MMP_RS07090 0.72 -4.5 1,357,006 - MMP_RS07090 0.72 -3.5 1,357,006 - MMP_RS07090 0.72 -3.5 1,357,006 - MMP_RS07090 0.72 -3.4 1,357,007 + MMP_RS07090 0.72 -5.2 1,357,007 + MMP_RS07090 0.72 -4.8 1,357,007 + MMP_RS07090 0.72 -2.4 1,357,015 - MMP_RS07090 0.73 -3.3 1,357,016 + MMP_RS07090 0.73 -3.6 1,357,071 - MMP_RS07090 0.79 -1.1 1,357,074 - MMP_RS07090 0.80 -5.2 1,357,075 + MMP_RS07090 0.80 -3.2 1,357,090 + MMP_RS07090 0.82 -3.4 1,357,090 + MMP_RS07090 0.82 -4.5 1,357,090 + MMP_RS07090 0.82 -4.0 1,357,094 - MMP_RS07090 0.82 -3.8 1,357,095 + MMP_RS07090 0.82 -3.2 1,357,159 - MMP_RS07090 0.89 -1.7 1,357,161 - MMP_RS07090 0.90 -5.8 1,357,164 - -3.6 1,357,165 + -3.1 1,357,298 - +0.3 1,357,302 + +0.0 1,357,323 - +0.7 1,357,341 + +1.0 1,357,388 + MMP_RS07095 0.19 +0.0 1,357,417 - MMP_RS07095 0.31 +1.1 1,357,417 - MMP_RS07095 0.31 +0.1 1,357,450 - MMP_RS07095 0.45 +4.1 1,357,487 - MMP_RS07095 0.60 +0.8 1,357,587 + +0.5 1,357,665 - +0.2 1,357,914 + MMP_RS07100 0.28 +0.5 1,357,955 - MMP_RS07100 0.32 +0.2 1,357,992 + MMP_RS07100 0.35 +0.2 1,357,994 + MMP_RS07100 0.35 -0.1 1,357,994 + MMP_RS07100 0.35 -0.2 1,357,996 - MMP_RS07100 0.36 -0.0 1,357,999 - MMP_RS07100 0.36 +0.2 1,358,061 - MMP_RS07100 0.42 -0.1 1,358,105 - MMP_RS07100 0.46 +0.7 1,358,177 - MMP_RS07100 0.53 -1.2 1,358,211 - MMP_RS07100 0.56 +0.4 1,358,212 + MMP_RS07100 0.56 -0.5 1,358,212 + MMP_RS07100 0.56 +0.6 1,358,213 - MMP_RS07100 0.56 -0.1 1,358,224 + MMP_RS07100 0.57 -0.1 1,358,357 - MMP_RS07100 0.69 -0.6 1,358,385 + MMP_RS07100 0.72 +0.6 1,358,429 - MMP_RS07100 0.76 +0.0 1,358,429 - MMP_RS07100 0.76 +0.5 1,358,430 + MMP_RS07100 0.76 -1.1 1,358,446 + MMP_RS07100 0.78 -0.3 1,358,446 + MMP_RS07100 0.78 +1.3 1,358,520 + MMP_RS07100 0.85 -0.5 1,358,570 + MMP_RS07100 0.89 +0.4
Or see this region's nucleotide sequence