Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06735 and MMP_RS06740 are separated by 51 nucleotides MMP_RS06740 and MMP_RS06745 are separated by 75 nucleotides MMP_RS06745 and MMP_RS06750 are separated by 35 nucleotides MMP_RS06750 and MMP_RS06755 are separated by 29 nucleotides MMP_RS06755 and MMP_RS06760 are separated by 17 nucleotides
MMP_RS06735: MMP_RS06735 - class I SAM-dependent rRNA methyltransferase, at 1,293,490 to 1,294,632
_RS06735
MMP_RS06740: MMP_RS06740 - fructose-6-phosphate aldolase, at 1,294,684 to 1,295,331
_RS06740
MMP_RS06745: MMP_RS06745 - DUF2119 domain-containing protein, at 1,295,407 to 1,296,018
_RS06745
MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659
_RS06750
MMP_RS06755: MMP_RS06755 - 4Fe-4S binding protein, at 1,296,689 to 1,296,847
_RS06755
MMP_RS06760: MMP_RS06760 - hypothetical protein, at 1,296,865 to 1,297,491
_RS06760
Position (kb)
1295
1296
1297 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1294.508 kb on - strand, within MMP_RS06735 at 1294.509 kb on + strand, within MMP_RS06735 at 1294.552 kb on + strand at 1294.604 kb on - strand at 1294.685 kb on - strand at 1295.089 kb on - strand at 1295.171 kb on + strand at 1295.240 kb on + strand at 1295.370 kb on + strand at 1295.385 kb on - strand at 1295.389 kb on - strand at 1295.390 kb on + strand at 1295.395 kb on + strand at 1295.395 kb on + strand at 1295.468 kb on - strand at 1295.472 kb on - strand, within MMP_RS06745 at 1295.539 kb on - strand, within MMP_RS06745 at 1295.659 kb on - strand, within MMP_RS06745 at 1295.660 kb on + strand, within MMP_RS06745 at 1295.940 kb on + strand, within MMP_RS06745 at 1296.147 kb on - strand at 1296.489 kb on + strand at 1296.655 kb on - strand at 1296.656 kb on + strand at 1296.729 kb on + strand at 1296.824 kb on - strand at 1296.861 kb on - strand at 1296.862 kb on + strand at 1296.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,294,508 - MMP_RS06735 0.89 +0.1 1,294,509 + MMP_RS06735 0.89 -0.1 1,294,552 + -0.4 1,294,604 - +0.3 1,294,685 - -0.1 1,295,089 - -3.8 1,295,171 + -3.1 1,295,240 + -3.5 1,295,370 + -3.3 1,295,385 - -0.1 1,295,389 - +0.4 1,295,390 + +0.6 1,295,395 + -0.0 1,295,395 + +0.0 1,295,468 - +0.3 1,295,472 - MMP_RS06745 0.11 -0.0 1,295,539 - MMP_RS06745 0.22 +0.4 1,295,659 - MMP_RS06745 0.41 +0.6 1,295,660 + MMP_RS06745 0.41 +1.0 1,295,940 + MMP_RS06745 0.87 +0.1 1,296,147 - -3.3 1,296,489 + -2.8 1,296,655 - -4.3 1,296,656 + -1.7 1,296,729 + -4.9 1,296,824 - -4.8 1,296,861 - -3.2 1,296,862 + -1.4 1,296,919 + -0.3
Or see this region's nucleotide sequence