Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06605 and MMP_RS06610 overlap by 4 nucleotides MMP_RS06610 and MMP_RS06615 are separated by 122 nucleotides MMP_RS06615 and MMP_RS06620 are separated by 18 nucleotides MMP_RS06620 and MMP_RS06625 are separated by 233 nucleotides
MMP_RS06605: MMP_RS06605 - DUF5814 domain-containing protein, at 1,265,602 to 1,268,037
_RS06605
MMP_RS06610: MMP_RS06610 - DUF2334 domain-containing protein, at 1,268,034 to 1,268,834
_RS06610
MMP_RS06615: MMP_RS06615 - tRNA-Gly, at 1,268,957 to 1,269,029
_RS06615
MMP_RS06620: MMP_RS06620 - tRNA-Gly, at 1,269,048 to 1,269,120
_RS06620
MMP_RS06625: MMP_RS06625 - DNA primase, at 1,269,354 to 1,270,664
_RS06625
Position (kb)
1268
1269 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1267.037 kb on - strand, within MMP_RS06605 at 1267.375 kb on + strand, within MMP_RS06605 at 1267.375 kb on + strand, within MMP_RS06605 at 1267.617 kb on - strand, within MMP_RS06605 at 1267.618 kb on + strand, within MMP_RS06605 at 1267.722 kb on + strand, within MMP_RS06605 at 1267.795 kb on + strand at 1267.910 kb on + strand at 1268.415 kb on + strand, within MMP_RS06610 at 1268.498 kb on - strand, within MMP_RS06610 at 1268.568 kb on + strand, within MMP_RS06610 at 1268.661 kb on + strand, within MMP_RS06610 at 1268.879 kb on - strand at 1268.890 kb on + strand at 1268.896 kb on + strand at 1268.897 kb on - strand at 1268.897 kb on - strand at 1268.898 kb on + strand at 1268.899 kb on - strand at 1268.899 kb on - strand at 1268.900 kb on + strand at 1269.245 kb on + strand at 1269.248 kb on - strand at 1269.248 kb on - strand at 1269.249 kb on + strand at 1269.249 kb on + strand at 1269.250 kb on - strand at 1269.251 kb on + strand at 1269.253 kb on + strand at 1269.269 kb on + strand at 1269.273 kb on + strand at 1269.273 kb on + strand at 1269.277 kb on + strand at 1269.292 kb on + strand at 1269.378 kb on + strand at 1269.602 kb on + strand, within MMP_RS06625 at 1269.760 kb on + strand, within MMP_RS06625
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,267,037 - MMP_RS06605 0.59 +0.1 1,267,375 + MMP_RS06605 0.73 +0.2 1,267,375 + MMP_RS06605 0.73 +0.1 1,267,617 - MMP_RS06605 0.83 +0.2 1,267,618 + MMP_RS06605 0.83 -0.3 1,267,722 + MMP_RS06605 0.87 -0.2 1,267,795 + -0.0 1,267,910 + -0.5 1,268,415 + MMP_RS06610 0.48 +0.3 1,268,498 - MMP_RS06610 0.58 +3.5 1,268,568 + MMP_RS06610 0.67 -0.4 1,268,661 + MMP_RS06610 0.78 +0.2 1,268,879 - +0.3 1,268,890 + -0.6 1,268,896 + -0.3 1,268,897 - +0.2 1,268,897 - +0.4 1,268,898 + -0.2 1,268,899 - -0.2 1,268,899 - +1.2 1,268,900 + -0.7 1,269,245 + +0.0 1,269,248 - -0.5 1,269,248 - -0.4 1,269,249 + +0.3 1,269,249 + +0.1 1,269,250 - +0.3 1,269,251 + -0.6 1,269,253 + -0.3 1,269,269 + -3.0 1,269,273 + -3.2 1,269,273 + -3.7 1,269,277 + -4.5 1,269,292 + -3.5 1,269,378 + -3.7 1,269,602 + MMP_RS06625 0.19 -4.0 1,269,760 + MMP_RS06625 0.31 -2.2
Or see this region's nucleotide sequence