Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06220 and MMP_RS06225 are separated by 36 nucleotides MMP_RS06225 and MMP_RS06230 are separated by 135 nucleotides MMP_RS06230 and MMP_RS06235 are separated by 62 nucleotides
MMP_RS06220: MMP_RS06220 - translation initiation factor IF-2 subunit gamma, at 1,194,834 to 1,196,066
_RS06220
MMP_RS06225: MMP_RS06225 - DNA double-strand break repair nuclease NurA, at 1,196,103 to 1,197,167
_RS06225
MMP_RS06230: MMP_RS06230 - helix-turn-helix domain-containing protein, at 1,197,303 to 1,197,992
_RS06230
MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104
_RS06235
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1195.513 kb on - strand at 1195.801 kb on - strand at 1196.138 kb on - strand at 1196.140 kb on - strand at 1196.140 kb on - strand at 1196.141 kb on + strand at 1196.141 kb on + strand at 1196.267 kb on - strand, within MMP_RS06225 at 1196.270 kb on + strand, within MMP_RS06225 at 1196.286 kb on + strand, within MMP_RS06225 at 1196.404 kb on + strand, within MMP_RS06225 at 1196.404 kb on + strand, within MMP_RS06225 at 1196.415 kb on + strand, within MMP_RS06225 at 1196.497 kb on - strand, within MMP_RS06225 at 1196.525 kb on - strand, within MMP_RS06225 at 1196.562 kb on - strand, within MMP_RS06225 at 1196.711 kb on + strand, within MMP_RS06225 at 1196.823 kb on + strand, within MMP_RS06225 at 1196.981 kb on - strand, within MMP_RS06225 at 1196.998 kb on - strand, within MMP_RS06225 at 1197.166 kb on + strand at 1197.170 kb on - strand at 1197.319 kb on - strand at 1197.499 kb on + strand, within MMP_RS06230 at 1197.502 kb on - strand, within MMP_RS06230 at 1197.502 kb on - strand, within MMP_RS06230 at 1197.504 kb on - strand, within MMP_RS06230 at 1197.505 kb on + strand, within MMP_RS06230 at 1197.543 kb on + strand, within MMP_RS06230 at 1197.764 kb on + strand, within MMP_RS06230 at 1197.928 kb on - strand at 1197.935 kb on - strand at 1198.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,195,513 - -2.8 1,195,801 - -3.2 1,196,138 - -0.2 1,196,140 - -0.3 1,196,140 - -0.2 1,196,141 + +0.1 1,196,141 + +3.3 1,196,267 - MMP_RS06225 0.15 +0.4 1,196,270 + MMP_RS06225 0.16 -0.0 1,196,286 + MMP_RS06225 0.17 -0.8 1,196,404 + MMP_RS06225 0.28 +0.3 1,196,404 + MMP_RS06225 0.28 -0.1 1,196,415 + MMP_RS06225 0.29 -0.2 1,196,497 - MMP_RS06225 0.37 +1.2 1,196,525 - MMP_RS06225 0.40 +4.2 1,196,562 - MMP_RS06225 0.43 +0.3 1,196,711 + MMP_RS06225 0.57 -0.6 1,196,823 + MMP_RS06225 0.68 +0.3 1,196,981 - MMP_RS06225 0.82 -0.2 1,196,998 - MMP_RS06225 0.84 +0.5 1,197,166 + +0.5 1,197,170 - +0.4 1,197,319 - -2.1 1,197,499 + MMP_RS06230 0.28 -2.8 1,197,502 - MMP_RS06230 0.29 -4.0 1,197,502 - MMP_RS06230 0.29 -2.2 1,197,504 - MMP_RS06230 0.29 -2.6 1,197,505 + MMP_RS06230 0.29 -3.0 1,197,543 + MMP_RS06230 0.35 -3.1 1,197,764 + MMP_RS06230 0.67 -3.6 1,197,928 - -1.5 1,197,935 - -4.1 1,198,075 + -2.8
Or see this region's nucleotide sequence