Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06225

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06220 and MMP_RS06225 are separated by 36 nucleotidesMMP_RS06225 and MMP_RS06230 are separated by 135 nucleotidesMMP_RS06230 and MMP_RS06235 are separated by 62 nucleotides MMP_RS06220: MMP_RS06220 - translation initiation factor IF-2 subunit gamma, at 1,194,834 to 1,196,066 _RS06220 MMP_RS06225: MMP_RS06225 - DNA double-strand break repair nuclease NurA, at 1,196,103 to 1,197,167 _RS06225 MMP_RS06230: MMP_RS06230 - helix-turn-helix domain-containing protein, at 1,197,303 to 1,197,992 _RS06230 MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104 _RS06235 Position (kb) 1196 1197 1198Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1195.513 kb on - strandat 1195.801 kb on - strandat 1196.138 kb on - strandat 1196.140 kb on - strandat 1196.140 kb on - strandat 1196.141 kb on + strandat 1196.141 kb on + strandat 1196.267 kb on - strand, within MMP_RS06225at 1196.270 kb on + strand, within MMP_RS06225at 1196.286 kb on + strand, within MMP_RS06225at 1196.404 kb on + strand, within MMP_RS06225at 1196.404 kb on + strand, within MMP_RS06225at 1196.415 kb on + strand, within MMP_RS06225at 1196.497 kb on - strand, within MMP_RS06225at 1196.525 kb on - strand, within MMP_RS06225at 1196.562 kb on - strand, within MMP_RS06225at 1196.711 kb on + strand, within MMP_RS06225at 1196.823 kb on + strand, within MMP_RS06225at 1196.981 kb on - strand, within MMP_RS06225at 1196.998 kb on - strand, within MMP_RS06225at 1197.166 kb on + strandat 1197.170 kb on - strandat 1197.319 kb on - strandat 1197.499 kb on + strand, within MMP_RS06230at 1197.502 kb on - strand, within MMP_RS06230at 1197.502 kb on - strand, within MMP_RS06230at 1197.504 kb on - strand, within MMP_RS06230at 1197.505 kb on + strand, within MMP_RS06230at 1197.543 kb on + strand, within MMP_RS06230at 1197.764 kb on + strand, within MMP_RS06230at 1197.928 kb on - strandat 1197.935 kb on - strandat 1198.075 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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1,195,513 - -2.8
1,195,801 - -3.2
1,196,138 - -0.2
1,196,140 - -0.3
1,196,140 - -0.2
1,196,141 + +0.1
1,196,141 + +3.3
1,196,267 - MMP_RS06225 0.15 +0.4
1,196,270 + MMP_RS06225 0.16 -0.0
1,196,286 + MMP_RS06225 0.17 -0.8
1,196,404 + MMP_RS06225 0.28 +0.3
1,196,404 + MMP_RS06225 0.28 -0.1
1,196,415 + MMP_RS06225 0.29 -0.2
1,196,497 - MMP_RS06225 0.37 +1.2
1,196,525 - MMP_RS06225 0.40 +4.2
1,196,562 - MMP_RS06225 0.43 +0.3
1,196,711 + MMP_RS06225 0.57 -0.6
1,196,823 + MMP_RS06225 0.68 +0.3
1,196,981 - MMP_RS06225 0.82 -0.2
1,196,998 - MMP_RS06225 0.84 +0.5
1,197,166 + +0.5
1,197,170 - +0.4
1,197,319 - -2.1
1,197,499 + MMP_RS06230 0.28 -2.8
1,197,502 - MMP_RS06230 0.29 -4.0
1,197,502 - MMP_RS06230 0.29 -2.2
1,197,504 - MMP_RS06230 0.29 -2.6
1,197,505 + MMP_RS06230 0.29 -3.0
1,197,543 + MMP_RS06230 0.35 -3.1
1,197,764 + MMP_RS06230 0.67 -3.6
1,197,928 - -1.5
1,197,935 - -4.1
1,198,075 + -2.8

Or see this region's nucleotide sequence