Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05950 and MMP_RS05955 are separated by 227 nucleotides MMP_RS05955 and MMP_RS05960 are separated by 12 nucleotides MMP_RS05960 and MMP_RS05965 are separated by 30 nucleotides
MMP_RS05950: MMP_RS05950 - nickel-dependent hydrogenase large subunit, at 1,141,444 to 1,142,571
_RS05950
MMP_RS05955: MMP_RS05955 - CoB--CoM heterodisulfide reductase subunit C, at 1,142,799 to 1,143,377
_RS05955
MMP_RS05960: MMP_RS05960 - CoB--CoM heterodisulfide reductase subunit B, at 1,143,390 to 1,144,295
_RS05960
MMP_RS05965: MMP_RS05965 - carboxymuconolactone decarboxylase family protein, at 1,144,326 to 1,144,640
_RS05965
Position (kb)
1142
1143
1144 Strain fitness (log2 ratio)
-1
0
1
2 at 1141.862 kb on - strand, within MMP_RS05950 at 1141.927 kb on - strand, within MMP_RS05950 at 1141.941 kb on - strand, within MMP_RS05950 at 1142.042 kb on - strand, within MMP_RS05950 at 1142.063 kb on + strand, within MMP_RS05950 at 1142.089 kb on + strand, within MMP_RS05950 at 1142.188 kb on + strand, within MMP_RS05950 at 1142.251 kb on + strand, within MMP_RS05950 at 1142.401 kb on + strand, within MMP_RS05950 at 1142.466 kb on - strand at 1142.504 kb on + strand at 1142.533 kb on + strand at 1142.568 kb on + strand at 1142.619 kb on + strand at 1142.636 kb on - strand at 1142.650 kb on + strand at 1142.659 kb on + strand at 1142.665 kb on - strand at 1142.675 kb on - strand at 1142.679 kb on - strand at 1142.679 kb on - strand at 1142.679 kb on - strand at 1142.680 kb on + strand at 1142.688 kb on + strand at 1142.723 kb on + strand at 1142.725 kb on - strand at 1142.727 kb on - strand at 1142.729 kb on - strand at 1142.729 kb on - strand at 1142.730 kb on + strand at 1142.730 kb on + strand at 1142.746 kb on - strand at 1142.746 kb on - strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.747 kb on + strand at 1142.748 kb on - strand at 1142.748 kb on - strand at 1142.749 kb on + strand at 1142.750 kb on - strand at 1142.751 kb on + strand at 1142.777 kb on - strand at 1143.032 kb on - strand, within MMP_RS05955 at 1143.098 kb on + strand, within MMP_RS05955 at 1143.486 kb on + strand, within MMP_RS05960 at 1143.675 kb on + strand, within MMP_RS05960 at 1143.709 kb on - strand, within MMP_RS05960 at 1143.742 kb on + strand, within MMP_RS05960 at 1143.744 kb on + strand, within MMP_RS05960 at 1143.769 kb on - strand, within MMP_RS05960 at 1143.778 kb on - strand, within MMP_RS05960 at 1143.779 kb on + strand, within MMP_RS05960 at 1143.783 kb on - strand, within MMP_RS05960 at 1143.856 kb on + strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.871 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.886 kb on - strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.887 kb on + strand, within MMP_RS05960 at 1143.958 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.960 kb on - strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.961 kb on + strand, within MMP_RS05960 at 1143.963 kb on - strand, within MMP_RS05960 at 1143.964 kb on + strand, within MMP_RS05960 at 1143.977 kb on - strand, within MMP_RS05960 at 1144.193 kb on + strand, within MMP_RS05960 at 1144.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,141,862 - MMP_RS05950 0.37 +0.7 1,141,927 - MMP_RS05950 0.43 +0.2 1,141,941 - MMP_RS05950 0.44 +1.1 1,142,042 - MMP_RS05950 0.53 +1.9 1,142,063 + MMP_RS05950 0.55 +0.8 1,142,089 + MMP_RS05950 0.57 +1.3 1,142,188 + MMP_RS05950 0.66 +1.4 1,142,251 + MMP_RS05950 0.72 +0.7 1,142,401 + MMP_RS05950 0.85 +1.4 1,142,466 - +1.1 1,142,504 + +1.4 1,142,533 + +1.0 1,142,568 + +0.9 1,142,619 + +1.2 1,142,636 - +1.9 1,142,650 + +1.5 1,142,659 + +1.3 1,142,665 - +1.0 1,142,675 - +1.2 1,142,679 - +1.1 1,142,679 - +0.9 1,142,679 - +1.7 1,142,680 + +0.8 1,142,688 + +0.7 1,142,723 + -0.1 1,142,725 - -0.3 1,142,727 - +0.2 1,142,729 - +0.3 1,142,729 - +0.3 1,142,730 + +0.7 1,142,730 + +0.4 1,142,746 - +0.1 1,142,746 - +0.5 1,142,747 + +0.2 1,142,747 + -0.4 1,142,747 + +0.5 1,142,747 + +0.2 1,142,748 - -0.9 1,142,748 - +0.2 1,142,749 + +0.7 1,142,750 - -0.2 1,142,751 + +0.1 1,142,777 - -0.3 1,143,032 - MMP_RS05955 0.40 +0.7 1,143,098 + MMP_RS05955 0.52 +0.5 1,143,486 + MMP_RS05960 0.11 +0.7 1,143,675 + MMP_RS05960 0.31 +0.3 1,143,709 - MMP_RS05960 0.35 +0.4 1,143,742 + MMP_RS05960 0.39 +0.4 1,143,744 + MMP_RS05960 0.39 -0.3 1,143,769 - MMP_RS05960 0.42 -0.3 1,143,778 - MMP_RS05960 0.43 +0.2 1,143,779 + MMP_RS05960 0.43 -1.4 1,143,783 - MMP_RS05960 0.43 +0.1 1,143,856 + MMP_RS05960 0.51 +0.1 1,143,871 - MMP_RS05960 0.53 +0.5 1,143,871 - MMP_RS05960 0.53 -0.0 1,143,886 - MMP_RS05960 0.55 -0.3 1,143,886 - MMP_RS05960 0.55 +0.3 1,143,887 + MMP_RS05960 0.55 +0.8 1,143,887 + MMP_RS05960 0.55 +0.6 1,143,958 - MMP_RS05960 0.63 +0.1 1,143,960 - MMP_RS05960 0.63 +0.1 1,143,960 - MMP_RS05960 0.63 +0.1 1,143,961 + MMP_RS05960 0.63 +0.2 1,143,961 + MMP_RS05960 0.63 +0.4 1,143,963 - MMP_RS05960 0.63 -0.4 1,143,964 + MMP_RS05960 0.63 -0.0 1,143,977 - MMP_RS05960 0.65 -0.0 1,144,193 + MMP_RS05960 0.89 +0.4 1,144,293 - -0.4
Or see this region's nucleotide sequence