Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05760 and MMP_RS05765 are separated by 338 nucleotides MMP_RS05765 and MMP_RS05770 are separated by 78 nucleotides MMP_RS05770 and MMP_RS05775 are separated by 4 nucleotides
MMP_RS05760: MMP_RS05760 - transketolase, at 1,104,891 to 1,105,718
_RS05760
MMP_RS05765: MMP_RS05765 - M42 family metallopeptidase, at 1,106,057 to 1,107,109
_RS05765
MMP_RS05770: MMP_RS05770 - YkgJ family cysteine cluster protein, at 1,107,188 to 1,107,676
_RS05770
MMP_RS05775: MMP_RS05775 - hypothetical protein, at 1,107,681 to 1,107,977
_RS05775
Position (kb)
1106
1107
1108 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1105.093 kb on + strand at 1105.394 kb on - strand at 1105.854 kb on - strand at 1105.867 kb on + strand at 1105.869 kb on - strand at 1105.869 kb on - strand at 1105.891 kb on - strand at 1105.992 kb on - strand at 1106.008 kb on - strand at 1106.008 kb on - strand at 1106.009 kb on + strand at 1106.009 kb on + strand at 1106.043 kb on + strand at 1106.125 kb on + strand at 1106.274 kb on - strand, within MMP_RS05765 at 1106.476 kb on - strand, within MMP_RS05765 at 1106.776 kb on - strand, within MMP_RS05765 at 1106.821 kb on - strand, within MMP_RS05765 at 1106.964 kb on - strand, within MMP_RS05765 at 1106.994 kb on - strand, within MMP_RS05765 at 1107.117 kb on - strand at 1107.123 kb on - strand at 1107.143 kb on + strand at 1107.236 kb on + strand at 1107.292 kb on - strand, within MMP_RS05770 at 1107.402 kb on - strand, within MMP_RS05770 at 1107.402 kb on - strand, within MMP_RS05770 at 1107.445 kb on - strand, within MMP_RS05770 at 1107.479 kb on + strand, within MMP_RS05770 at 1107.503 kb on - strand, within MMP_RS05770 at 1107.504 kb on + strand, within MMP_RS05770 at 1107.553 kb on + strand, within MMP_RS05770 at 1107.574 kb on - strand, within MMP_RS05770 at 1107.574 kb on - strand, within MMP_RS05770 at 1107.575 kb on + strand, within MMP_RS05770 at 1108.017 kb on - strand at 1108.018 kb on + strand at 1108.018 kb on + strand at 1108.020 kb on + strand at 1108.041 kb on - strand at 1108.042 kb on + strand at 1108.042 kb on + strand at 1108.046 kb on + strand at 1108.056 kb on - strand at 1108.057 kb on + strand at 1108.059 kb on + strand at 1108.078 kb on + strand at 1108.103 kb on - strand at 1108.103 kb on - strand at 1108.104 kb on + strand at 1108.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,105,093 + -1.3 1,105,394 - -1.2 1,105,854 - +0.6 1,105,867 + -3.5 1,105,869 - -0.7 1,105,869 - +0.5 1,105,891 - +0.2 1,105,992 - +0.4 1,106,008 - -0.4 1,106,008 - -0.3 1,106,009 + +0.1 1,106,009 + +0.6 1,106,043 + -1.0 1,106,125 + -1.3 1,106,274 - MMP_RS05765 0.21 -2.0 1,106,476 - MMP_RS05765 0.40 -1.6 1,106,776 - MMP_RS05765 0.68 -1.5 1,106,821 - MMP_RS05765 0.73 -1.6 1,106,964 - MMP_RS05765 0.86 -1.2 1,106,994 - MMP_RS05765 0.89 -1.5 1,107,117 - -0.5 1,107,123 - +0.2 1,107,143 + -0.2 1,107,236 + -0.2 1,107,292 - MMP_RS05770 0.21 +0.3 1,107,402 - MMP_RS05770 0.44 -0.2 1,107,402 - MMP_RS05770 0.44 +0.2 1,107,445 - MMP_RS05770 0.53 +0.0 1,107,479 + MMP_RS05770 0.60 -0.4 1,107,503 - MMP_RS05770 0.64 +0.2 1,107,504 + MMP_RS05770 0.65 +0.3 1,107,553 + MMP_RS05770 0.75 +0.6 1,107,574 - MMP_RS05770 0.79 -0.0 1,107,574 - MMP_RS05770 0.79 +0.5 1,107,575 + MMP_RS05770 0.79 -0.1 1,108,017 - +0.3 1,108,018 + +0.2 1,108,018 + +0.8 1,108,020 + +0.5 1,108,041 - -0.1 1,108,042 + +0.6 1,108,042 + +0.1 1,108,046 + -0.1 1,108,056 - -0.1 1,108,057 + +0.1 1,108,059 + +0.1 1,108,078 + -0.1 1,108,103 - +0.5 1,108,103 - +0.1 1,108,104 + -0.4 1,108,106 + -0.1
Or see this region's nucleotide sequence