Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05535 and MMP_RS05540 are separated by 95 nucleotides MMP_RS05540 and MMP_RS05545 are separated by 14 nucleotides MMP_RS05545 and MMP_RS05550 are separated by 199 nucleotides MMP_RS05550 and MMP_RS05555 are separated by 9 nucleotides MMP_RS05555 and MMP_RS05560 are separated by 70 nucleotides
MMP_RS05535: MMP_RS05535 - hypothetical protein, at 1,062,398 to 1,063,015
_RS05535
MMP_RS05540: MMP_RS05540 - hypothetical protein, at 1,063,111 to 1,063,530
_RS05540
MMP_RS05545: MMP_RS05545 - pyridoxal phosphate-dependent aminotransferase, at 1,063,545 to 1,064,672
_RS05545
MMP_RS05550: MMP_RS05550 - cation:proton antiporter, at 1,064,872 to 1,065,126
_RS05550
MMP_RS05555: MMP_RS05555 - DUF4040 domain-containing protein, at 1,065,136 to 1,065,378
_RS05555
MMP_RS05560: MMP_RS05560 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 1,065,449 to 1,065,919
_RS05560
Position (kb)
1063
1064
1065 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1062.912 kb on + strand, within MMP_RS05535 at 1062.912 kb on + strand, within MMP_RS05535 at 1062.999 kb on - strand at 1063.000 kb on + strand at 1063.005 kb on + strand at 1063.008 kb on - strand at 1063.008 kb on - strand at 1063.008 kb on - strand at 1063.033 kb on - strand at 1063.033 kb on - strand at 1063.034 kb on + strand at 1063.041 kb on - strand at 1063.041 kb on - strand at 1063.041 kb on - strand at 1063.041 kb on - strand at 1063.042 kb on + strand at 1063.042 kb on + strand at 1063.042 kb on + strand at 1063.042 kb on + strand at 1063.061 kb on + strand at 1063.071 kb on + strand at 1063.097 kb on + strand at 1063.154 kb on + strand, within MMP_RS05540 at 1063.189 kb on + strand, within MMP_RS05540 at 1063.298 kb on + strand, within MMP_RS05540 at 1063.398 kb on - strand, within MMP_RS05540 at 1063.418 kb on - strand, within MMP_RS05540 at 1063.419 kb on + strand, within MMP_RS05540 at 1063.419 kb on + strand, within MMP_RS05540 at 1063.419 kb on + strand, within MMP_RS05540 at 1063.532 kb on - strand at 1063.573 kb on + strand at 1063.584 kb on - strand at 1063.735 kb on - strand, within MMP_RS05545 at 1063.736 kb on + strand, within MMP_RS05545 at 1063.763 kb on - strand, within MMP_RS05545 at 1063.763 kb on - strand, within MMP_RS05545 at 1063.769 kb on - strand, within MMP_RS05545 at 1063.851 kb on + strand, within MMP_RS05545 at 1063.943 kb on - strand, within MMP_RS05545 at 1064.087 kb on + strand, within MMP_RS05545 at 1064.684 kb on + strand at 1064.688 kb on + strand at 1064.691 kb on - strand at 1064.692 kb on + strand at 1064.694 kb on + strand at 1064.694 kb on + strand at 1064.695 kb on - strand at 1064.695 kb on - strand at 1064.695 kb on - strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.696 kb on + strand at 1064.709 kb on + strand at 1064.709 kb on + strand at 1064.718 kb on + strand at 1064.751 kb on - strand at 1064.754 kb on - strand at 1064.754 kb on - strand at 1064.754 kb on - strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.755 kb on + strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.756 kb on - strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.757 kb on + strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.758 kb on - strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.759 kb on + strand at 1064.762 kb on - strand at 1064.763 kb on + strand at 1064.763 kb on + strand at 1064.763 kb on + strand at 1064.777 kb on + strand at 1064.784 kb on - strand at 1064.858 kb on - strand at 1064.859 kb on + strand at 1065.006 kb on - strand, within MMP_RS05550 at 1065.213 kb on + strand, within MMP_RS05555 at 1065.276 kb on + strand, within MMP_RS05555 at 1065.376 kb on - strand at 1065.381 kb on - strand at 1065.387 kb on - strand at 1065.457 kb on - strand at 1065.457 kb on - strand at 1065.463 kb on + strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.474 kb on - strand at 1065.528 kb on - strand, within MMP_RS05560 at 1065.528 kb on - strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.533 kb on + strand, within MMP_RS05560 at 1065.535 kb on + strand, within MMP_RS05560 at 1065.555 kb on + strand, within MMP_RS05560 at 1065.607 kb on - strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.608 kb on + strand, within MMP_RS05560 at 1065.610 kb on + strand, within MMP_RS05560
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,062,912 + MMP_RS05535 0.83 -0.1 1,062,912 + MMP_RS05535 0.83 -0.4 1,062,999 - -0.0 1,063,000 + -0.3 1,063,005 + -3.0 1,063,008 - -0.7 1,063,008 - -0.8 1,063,008 - -1.7 1,063,033 - -0.9 1,063,033 - +3.9 1,063,034 + -0.8 1,063,041 - +0.8 1,063,041 - +0.6 1,063,041 - -0.2 1,063,041 - +0.8 1,063,042 + -0.1 1,063,042 + +0.3 1,063,042 + +1.2 1,063,042 + +1.0 1,063,061 + -0.2 1,063,071 + +1.0 1,063,097 + +0.6 1,063,154 + MMP_RS05540 0.10 +0.7 1,063,189 + MMP_RS05540 0.19 +0.9 1,063,298 + MMP_RS05540 0.45 -3.9 1,063,398 - MMP_RS05540 0.68 -3.1 1,063,418 - MMP_RS05540 0.73 -4.1 1,063,419 + MMP_RS05540 0.73 -3.7 1,063,419 + MMP_RS05540 0.73 -2.7 1,063,419 + MMP_RS05540 0.73 -3.8 1,063,532 - -3.4 1,063,573 + -0.5 1,063,584 - +0.8 1,063,735 - MMP_RS05545 0.17 +0.1 1,063,736 + MMP_RS05545 0.17 +0.1 1,063,763 - MMP_RS05545 0.19 +0.6 1,063,763 - MMP_RS05545 0.19 +0.3 1,063,769 - MMP_RS05545 0.20 +0.5 1,063,851 + MMP_RS05545 0.27 +0.5 1,063,943 - MMP_RS05545 0.35 +0.8 1,064,087 + MMP_RS05545 0.48 +0.1 1,064,684 + +0.6 1,064,688 + -0.3 1,064,691 - +0.8 1,064,692 + +0.4 1,064,694 + +0.1 1,064,694 + -0.6 1,064,695 - -1.9 1,064,695 - -2.8 1,064,695 - -2.1 1,064,696 + -2.1 1,064,696 + -0.6 1,064,696 + -1.4 1,064,696 + -0.4 1,064,709 + -2.3 1,064,709 + -1.0 1,064,718 + -2.1 1,064,751 - -4.5 1,064,754 - -5.1 1,064,754 - -3.3 1,064,754 - -2.0 1,064,755 + -1.0 1,064,755 + -1.6 1,064,755 + -1.9 1,064,755 + -1.5 1,064,756 - -4.1 1,064,756 - -4.9 1,064,756 - -3.1 1,064,756 - -3.5 1,064,756 - -3.2 1,064,757 + -1.5 1,064,757 + -1.5 1,064,757 + -1.1 1,064,757 + -1.6 1,064,757 + -2.2 1,064,757 + -1.4 1,064,758 - -3.3 1,064,758 - -3.2 1,064,758 - -3.3 1,064,758 - -3.5 1,064,758 - -3.4 1,064,758 - -1.8 1,064,759 + -0.9 1,064,759 + -0.8 1,064,759 + -0.2 1,064,759 + -1.5 1,064,759 + -1.5 1,064,759 + -1.2 1,064,759 + -1.8 1,064,759 + -1.6 1,064,759 + -1.1 1,064,762 - -3.1 1,064,763 + -0.7 1,064,763 + -1.1 1,064,763 + -0.3 1,064,777 + -1.9 1,064,784 - -2.5 1,064,858 - -2.4 1,064,859 + -0.9 1,065,006 - MMP_RS05550 0.53 -2.8 1,065,213 + MMP_RS05555 0.32 -3.3 1,065,276 + MMP_RS05555 0.58 -3.3 1,065,376 - +0.5 1,065,381 - -0.0 1,065,387 - +0.2 1,065,457 - -0.8 1,065,457 - -0.0 1,065,463 + +0.4 1,065,474 - +0.3 1,065,474 - +0.2 1,065,474 - +0.5 1,065,528 - MMP_RS05560 0.17 -0.0 1,065,528 - MMP_RS05560 0.17 +0.1 1,065,533 + MMP_RS05560 0.18 -0.9 1,065,533 + MMP_RS05560 0.18 -0.1 1,065,535 + MMP_RS05560 0.18 -0.2 1,065,555 + MMP_RS05560 0.23 -0.5 1,065,607 - MMP_RS05560 0.34 +0.5 1,065,608 + MMP_RS05560 0.34 -0.6 1,065,608 + MMP_RS05560 0.34 -0.4 1,065,610 + MMP_RS05560 0.34 -0.2
Or see this region's nucleotide sequence