Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05220 and MMP_RS05225 are separated by 24 nucleotides MMP_RS05225 and MMP_RS05230 are separated by 110 nucleotides
MMP_RS05220: MMP_RS05220 - dihydroorotase, at 993,042 to 994,298
_RS05220
MMP_RS05225: MMP_RS05225 - RluA family pseudouridine synthase, at 994,323 to 995,216
_RS05225
MMP_RS05230: MMP_RS05230 - glutamate--tRNA ligase, at 995,327 to 996,994
_RS05230
Position (kb) 994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 993.726 kb on + strand at 993.744 kb on + strand at 993.747 kb on - strand at 993.892 kb on - strand at 994.352 kb on - strand at 994.353 kb on + strand at 994.374 kb on + strand at 994.439 kb on - strand, within MMP_RS05225 at 994.439 kb on - strand, within MMP_RS05225 at 994.451 kb on - strand, within MMP_RS05225 at 994.451 kb on - strand, within MMP_RS05225 at 994.485 kb on + strand, within MMP_RS05225 at 994.504 kb on + strand, within MMP_RS05225 at 994.536 kb on + strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.539 kb on - strand, within MMP_RS05225 at 994.540 kb on + strand, within MMP_RS05225 at 994.588 kb on - strand, within MMP_RS05225 at 994.592 kb on - strand, within MMP_RS05225 at 994.699 kb on + strand, within MMP_RS05225 at 994.709 kb on - strand, within MMP_RS05225 at 994.709 kb on - strand, within MMP_RS05225 at 994.710 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.712 kb on + strand, within MMP_RS05225 at 994.713 kb on - strand, within MMP_RS05225 at 994.765 kb on - strand, within MMP_RS05225 at 994.766 kb on + strand, within MMP_RS05225 at 994.932 kb on + strand, within MMP_RS05225 at 994.960 kb on - strand, within MMP_RS05225 at 994.971 kb on - strand, within MMP_RS05225 at 994.972 kb on + strand, within MMP_RS05225 at 995.218 kb on - strand at 995.219 kb on + strand at 995.219 kb on + strand at 995.259 kb on - strand at 995.274 kb on - strand at 995.320 kb on - strand at 995.322 kb on - strand at 995.339 kb on - strand at 995.353 kb on + strand at 995.353 kb on + strand at 995.356 kb on - strand at 995.670 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 993,726 + -2.9 993,744 + -2.1 993,747 - -3.3 993,892 - -3.3 994,352 - +0.7 994,353 + -0.3 994,374 + +0.8 994,439 - MMP_RS05225 0.13 -0.3 994,439 - MMP_RS05225 0.13 -0.0 994,451 - MMP_RS05225 0.14 +0.4 994,451 - MMP_RS05225 0.14 +0.3 994,485 + MMP_RS05225 0.18 +0.8 994,504 + MMP_RS05225 0.20 +0.1 994,536 + MMP_RS05225 0.24 -0.0 994,539 - MMP_RS05225 0.24 +0.8 994,539 - MMP_RS05225 0.24 -0.2 994,539 - MMP_RS05225 0.24 +1.5 994,540 + MMP_RS05225 0.24 +1.7 994,588 - MMP_RS05225 0.30 +0.3 994,592 - MMP_RS05225 0.30 -0.3 994,699 + MMP_RS05225 0.42 +0.3 994,709 - MMP_RS05225 0.43 -0.2 994,709 - MMP_RS05225 0.43 -0.0 994,710 + MMP_RS05225 0.43 +0.5 994,712 + MMP_RS05225 0.44 -0.2 994,712 + MMP_RS05225 0.44 +0.7 994,712 + MMP_RS05225 0.44 +0.7 994,713 - MMP_RS05225 0.44 -0.2 994,765 - MMP_RS05225 0.49 +0.1 994,766 + MMP_RS05225 0.50 +0.9 994,932 + MMP_RS05225 0.68 -0.3 994,960 - MMP_RS05225 0.71 +3.0 994,971 - MMP_RS05225 0.72 -0.4 994,972 + MMP_RS05225 0.73 +0.2 995,218 - -0.6 995,219 + +0.4 995,219 + +0.0 995,259 - +0.3 995,274 - +0.5 995,320 - -0.0 995,322 - +0.2 995,339 - +0.0 995,353 + +0.3 995,353 + -2.0 995,356 - -0.9 995,670 + -3.0
Or see this region's nucleotide sequence